Fluorescence Characteristics of Hoechst 33258 Complexes with Single-Stranded and Double-Stranded Polyribonucleotides in Solution at Various Solution Ionic Strengths

IF 1 4区化学 Q4 SPECTROSCOPY Journal of Applied Spectroscopy Pub Date : 2025-01-16 DOI:10.1007/s10812-025-01850-z

A. P. Antonyan, P. O. Vardevanyan, M. S. Mikaelyan, G. H. Poghosyan, G. H. Kocharyan, M. A. Parsadanyan

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Abstract

A fluorescence spectroscopy study has been carried out on the interaction of Hoechst 33258 (H33258) with single-stranded (ss-)polyribonucleotides poly(rA) and poly(rU) and double-stranded (ds)-poly(rA)–poly(rU). Groove-binding compound H33258, which is a specific ligand for ds-nucleic acids (ds-NA), can also bind to ss-RNA. H33258 shows higher preference for poly(rA)–poly(rU) formed due to hybridization than for ss-poly(rA) and ss-poly(rU). Fluorescence spectroscopy was used to construct the binding curves of H33258 to ss- and dspolyribonucleotides. The binding constants K and number of bases per binding site n were determined. The data obtained indicated that the binding constant of H33258 to ss-poly(rA) is 4–5 times higher than that for sspoly(rU). The affinity of H33258 for poly(rA)–poly(rU) and ss-poly(rA) was found to depend on the ionic strength of the solution, while such a dependence is not found for ss-poly(rU).

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不同溶液离子强度下Hoechst 33258单链和双链多核糖核苷酸配合物的荧光特性

利用荧光光谱研究了Hoechst 33258 （H33258）与单链（ss-）多核糖核苷酸poly（rA）和poly（rU）以及双链(ds)-poly(rA) -poly（rU）的相互作用。凹槽结合化合物H33258是ds-核酸（ds-NA）的特异性配体，也可以与ss-RNA结合。与ss-poly（rA）和ss-poly（rU）相比，H33258对杂化形成的poly(rA) -poly（rU）表现出更高的偏好。荧光光谱法构建了H33258与单链和多聚核糖核苷酸的结合曲线。测定了结合常数K和每个结合位点n的碱基数。结果表明，H33258与ss-poly（rA）的结合常数比与ss-poly（rU）的结合常数高4-5倍。H33258对poly(rA) -poly（rU）和ss-poly（rA）的亲和性取决于溶液的离子强度，而对ss-poly（rU）没有这种依赖性。

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来源期刊

Journal of Applied Spectroscopy SPECTROSCOPY-

CiteScore

1.30

自引率

14.30%

发文量

145

审稿时长

2.5 months

期刊介绍： Journal of Applied Spectroscopy reports on many key applications of spectroscopy in chemistry, physics, metallurgy, and biology. An increasing number of papers focus on the theory of lasers, as well as the tremendous potential for the practical applications of lasers in numerous fields and industries.