{"title":"β-cyclodextrin inclusion complex as a potent delivery system for enhanced cytotoxicity of paclitaxel in triple-negative breast cancer cells","authors":"Kamini Velhal, Parvindar Sah, Smitali Patil, Rajesh Raut, Ramesh Yamgar, Jaya Lakkakula, Imran Uddin","doi":"10.1007/s11051-025-06229-x","DOIUrl":null,"url":null,"abstract":"<div><p>The toxicity of drugs to normal cells exhibits a significant risk to human health. Currently, researchers worldwide are striving to develop custom-designed drugs that have reduced toxicity and fewer side effects. Polymeric nanoparticles have drawn interest in being a suitable anticancer drug carrier and molecular visualization. Paclitaxel (PTX) has great potential as an anticancer drug though it suffers from poor aqueous solubility, limiting its therapeutic efficacy. This study investigated a novel β-cyclodextrin (β-CD) inclusion complex for enhanced PTX delivery against triple-negative human breast cancer (aka. MDA-MB-231). Molecular docking simulations indicated the strongest binding between β-CD and PTX compared to α-CD and γ-CD. The 1:2 molar ratio of β-CD to PTX achieved the highest entrapment efficiency (over 95.23%) and improved PTX solubility. Characterization techniques confirmed the successful formation of inclusion complex PTX-β-CD (IC). The IC exhibited critical cytotoxicity against MDA-MB-231. It diminishes colony formation and hampers the migration of cells. Evaluation of cellular morphology by apoptosis assays demonstrated the formulations’ impact on both the cytoskeleton and cytotoxicity of MDA-MB-231, potentially inducing apoptosis. The synthesized IC shows significant potential for pharmaceutical development.</p></div>","PeriodicalId":653,"journal":{"name":"Journal of Nanoparticle Research","volume":"27 2","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanoparticle Research","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s11051-025-06229-x","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The toxicity of drugs to normal cells exhibits a significant risk to human health. Currently, researchers worldwide are striving to develop custom-designed drugs that have reduced toxicity and fewer side effects. Polymeric nanoparticles have drawn interest in being a suitable anticancer drug carrier and molecular visualization. Paclitaxel (PTX) has great potential as an anticancer drug though it suffers from poor aqueous solubility, limiting its therapeutic efficacy. This study investigated a novel β-cyclodextrin (β-CD) inclusion complex for enhanced PTX delivery against triple-negative human breast cancer (aka. MDA-MB-231). Molecular docking simulations indicated the strongest binding between β-CD and PTX compared to α-CD and γ-CD. The 1:2 molar ratio of β-CD to PTX achieved the highest entrapment efficiency (over 95.23%) and improved PTX solubility. Characterization techniques confirmed the successful formation of inclusion complex PTX-β-CD (IC). The IC exhibited critical cytotoxicity against MDA-MB-231. It diminishes colony formation and hampers the migration of cells. Evaluation of cellular morphology by apoptosis assays demonstrated the formulations’ impact on both the cytoskeleton and cytotoxicity of MDA-MB-231, potentially inducing apoptosis. The synthesized IC shows significant potential for pharmaceutical development.
期刊介绍：
The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size.
Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology.
The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.
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