Neurovascular coupling response: A follow-up study

IF 2.8 3区医学 Q1 OPHTHALMOLOGY Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.17276

João Jordão, Sérgio Rodrigues, Joana Domingues, Pedro Serranho, Pedro Guimarães, Rui Bernardes

{"title":"Neurovascular coupling response: A follow-up study","authors":"João Jordão, Sérgio Rodrigues, Joana Domingues, Pedro Serranho, Pedro Guimarães, Rui Bernardes","doi":"10.1111/aos.17276","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n Aims/Purpose: This work aims to assess the consistency of the neurovascular coupling (NVC) response of a healthy subject group at two visits 5 months apart.\n \n Methods: Fundus images (4x4 degrees) of the macular region (3 baseline acquisitions and 3 acquisitions each preceded by flicker stimulation) of 13 healthy adults (one eye each, age (m+/-sd): 33.08+/-11.4 yrs.) were acquired with the rtx1 AO camera (ImagineEyes, Orsay, France). The rtx1 was configured for a 20 second, 15Hz, and 4 μW.cm^(-2) flicker stimulation over a 29x20 degrees area of the imaged eye fundus. Acquisitions for each subject were repeated 159+/-17 (m+/-sd) days later.\n \n The lumen of retinal arterioles within the macular region was determined using custom-developed software for the automatic segmentation of lumen-wall interfaces. An experienced technician visually assessed all segmentations to reject subpar results. Lumen diameters were calculated from the distance between the lumen-wall interfaces as measured perpendicular to the vessel axis along a vessel segment. NVC responses were calculated per eye as the relative difference (%) of the lumen diameter after stimulation to the respective baseline.\n \n Results: A total of 22 out of 156 images (14%) were rejected and excluded from further analysis. NVC responses ranged from 0.21% to 8.28% (3.36+/-2.75 % (m+/-sd)) for the first visit and from -0.24% to 9.13% (3.03+/-2.54 %) for the follow-up. Response differences between time points ranged from -6.15% (8.28% to 2.13%) to 4.21% (0.98% to 5.19%), with an average (sd) of 0.34(3.03) % for the group. A paired sample Wilcoxon test between time points is not statistically significant (p = 0.650), supporting that the responses are consistent between time points.\n \n Conclusions: The obtained results show a consistent NVC response for a 5-month interval.\n </section>\n </div>","PeriodicalId":6915,"journal":{"name":"Acta Ophthalmologica","volume":"103 S284","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/10.1111/aos.17276","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Ophthalmologica","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aos.17276","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aims/Purpose: This work aims to assess the consistency of the neurovascular coupling (NVC) response of a healthy subject group at two visits 5 months apart.

Methods: Fundus images (4x4 degrees) of the macular region (3 baseline acquisitions and 3 acquisitions each preceded by flicker stimulation) of 13 healthy adults (one eye each, age (m+/-sd): 33.08+/-11.4 yrs.) were acquired with the rtx1 AO camera (ImagineEyes, Orsay, France). The rtx1 was configured for a 20 second, 15Hz, and 4 μW.cm^(-2) flicker stimulation over a 29x20 degrees area of the imaged eye fundus. Acquisitions for each subject were repeated 159+/-17 (m+/-sd) days later.

The lumen of retinal arterioles within the macular region was determined using custom-developed software for the automatic segmentation of lumen-wall interfaces. An experienced technician visually assessed all segmentations to reject subpar results. Lumen diameters were calculated from the distance between the lumen-wall interfaces as measured perpendicular to the vessel axis along a vessel segment. NVC responses were calculated per eye as the relative difference (%) of the lumen diameter after stimulation to the respective baseline.

Results: A total of 22 out of 156 images (14%) were rejected and excluded from further analysis. NVC responses ranged from 0.21% to 8.28% (3.36+/-2.75 % (m+/-sd)) for the first visit and from -0.24% to 9.13% (3.03+/-2.54 %) for the follow-up. Response differences between time points ranged from -6.15% (8.28% to 2.13%) to 4.21% (0.98% to 5.19%), with an average (sd) of 0.34(3.03) % for the group. A paired sample Wilcoxon test between time points is not statistically significant (p = 0.650), supporting that the responses are consistent between time points.

Conclusions: The obtained results show a consistent NVC response for a 5-month interval.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

神经血管耦合反应：一项随访研究

目的/目的：本研究旨在评估健康受试者间隔5个月两次访视时神经血管耦合（NVC）反应的一致性。方法：使用rtx1型AO相机（ImagineEyes, Orsay, France）获取13例健康成人黄斑区域眼底图像（4 × 4度）（3次基线采集和3次闪烁刺激前采集）（每只眼，年龄（m+/-sd）： 33.08+/-11.4岁）。rtx1在成像的眼底29x20度区域进行20秒，15Hz和4 μW.cm^（-2）的闪烁刺激。每个受试者的采集在159+/-17 （m+/-sd）天后重复。黄斑区域内视网膜小动脉的管腔使用定制开发的管腔-壁界面自动分割软件进行确定。经验丰富的技术人员直观地评估所有分割，以拒绝不合格的结果。管腔直径是根据管腔壁界面之间的距离计算出来的，管腔壁界面沿着血管段垂直于血管轴线测量。每只眼的NVC反应计算为刺激后管腔直径与相应基线的相对差值（%）。结果：156张图像中有22张（14%）被拒绝，并被排除在进一步的分析之外。首次访视的NVC应答从0.21%到8.28% (3.36+/- 2.75% (m+/-sd))，随访的NVC应答从-0.24%到9.13%（3.03+/- 2.54%）。时间点间反应差异为-6.15%(8.28% ~ 2.13%)~ 4.21%(0.98% ~ 5.19%)，组平均sd为0.34(3.03)%。时间点之间的配对样本Wilcoxon检验没有统计学意义（p = 0.650），支持时间点之间的反应是一致的。结论：获得的结果显示连续5个月的NVC应答。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.