Morphological and molecular characterization of avian trypanosomes in domestic chickens (Gallus gallus domesticus) in Southeast Asia and review of the parasite morphometry in different avian hosts.

IF 2.2 2区 农林科学 Q1 VETERINARY SCIENCES Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-02-24 DOI:10.1080/03079457.2025.2462628
Pornchai Pornpanom, Gediminas Valkiūnas, Surya Paudel
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Abstract

Research highlights: The prevalence of trypanosomes in chickens is high.The first report of Trypanosoma avium in chickens with evidence of species identification.The first report of morphological and molecular characteristics of Trypanosoma calmettei.A cheap and quick buffy coat method is helpful for screening trypanosomes.

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东南亚家鸡(Gallus Gallus domesticus)禽锥虫的形态和分子特征及其在不同鸟类宿主中的形态研究进展。
摘要锥虫病是由锥虫属鞭毛原生生物引起的。这些寄生虫通过各种吸血节肢动物传播。虽然这些寄生虫通常被认为具有低致病性,但某些物种可在易感鸟类中引起临床症状。本研究对泰国东北部家鸡(Gallus Gallus domesticus)中分离的锥虫进行了详细的形态学和分子特征研究。共分析了58张白皮毛血膜、60张新鲜血液薄血膜和60张部分DNA序列。随后,对禽类中罕见的鸟锥虫和卡氏锥虫以及锥虫属进行了鉴定。此外,我们还提供了不同鸟类宿主中寄生虫形态测定的详细资料。系统发育分析确定了亲缘关系最密切的禽锥虫谱系。本研究报道了家鸡的鸟绦虫及其分子特征,这是一种很少报道的物种。巴色被法和巢式pcr诊断方法对锥虫的诊断灵敏度几乎相似,且两种方法都比血膜镜检更敏感。相对便宜的黄皮毛法有助于禽类锥虫感染的筛查,特别是在快速实地调查中。在寄生虫学研究中,黄皮毛和巢式pcr诊断与薄血涂片镜检相结合,可为非变形锥乳虫提供有价值的信息,对形态种鉴定至关重要。
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来源期刊
Avian Pathology
Avian Pathology 农林科学-兽医学
CiteScore
4.50
自引率
10.70%
发文量
68
审稿时长
1 months
期刊介绍: Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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