Hsa_circ_0030568 Promotes the Proliferation and Migration of Prostate Cancer Cells via the miR-141-3p-BRD4 Axis.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Combinatorial chemistry & high throughput screening Pub Date : 2025-01-30 DOI:10.2174/0113862073341224241228054703

Xiaoxiao Sun, Congming Xiang, Yuwei Wu, Dongsheng Ge, Chengwen Sun

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Abstract

Introduction: Numerous studies have indicated that circular RNAs (circRNAs) play an important role in diverse cancers. However, the specific molecular mechanism of circRNAs in prostate cancer (PCa) remains largely unclear.

Method: In this study, we performed western blotting, real-time quantitative polymerase chain reaction, CCK-8 assay, cell scratch wound assay, and RNA immunoprecipitation assay.

Results: These results showed that the expression of hsa_circ_0030568 was remarkably increased in PCa cells by using quantitative real-time PCR combined with dataset analysis. Mechanistically, circ_0030568 could physically bind to miR-141-3p and elevate the expression of BRD4 via sponging miR-141-3p in PCa cells, then accelerating the progression of PCa. Upregulation of hsa_circ_0030568 induces tumor progression through the miR-141-3p-BRD4 axis.

Conclusion: Taken together, these findings suggested that hsa_circ_0030568 may act as a potential biomarker of PCa and a promising target for PCa treatment.

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.