A comparison of the proto-dolomite induced by cyanobacteria and halophilic bacteria: implications for dolomite-inducing microbe identification

Abstract

This study investigates the morphological and mineralogical characteristics of proto-dolomite induced by two specific microorganisms with varying lifestyles: the extremely halophilic bacterium Vibrio harveyi QPL2 and the cyanobacterium Leptolyngbya boryana. Halophilic bacterially-induced proto-dolomite (HBD) and cyanobacterially-induced proto-dolomite (CBD) were subjected to comprehensive characterization techniques, including X-ray diffraction, scanning electron microscopy, Focused Ion Beam, thermogravimetric analysis, and X-ray photoelectron spectroscopy. The results indicate that both HBD and CBD exhibit a low degree of crystallinity and possess comparable molar ratios of MgCO₃ to CaCO₃. Moreover, neither of them exhibits the ordered structure of ideal dolomite. HBD and CBD exhibit notable distinctions in external morphology and internal structure. HBD forms a subunit aggregate with a less dense surface and numerous pinhole structures resulting from bacterial survival. In contrast, CBD adopts a bispherical shape with a relatively dense surface and minimal indications of cyanobacterial survival. Both HBD and CBD have an internal hollow structure. However, HBD is characterized by sparse population and loosely arranged subunits, while CBD features only a central cavity. Additionally, HBD particles are smaller compared to CBD particles. These morphological differences suggest that HBD primarily grows through bacterial surface-dependent processes, whereas the growth of CBD is not directly reliant on the surface of cyanobacteria. Compositionally, the weight percentage of crystalline water in CBD exceeded that of HBD with a value of 29.42 % compared to 5.9 %. This increase in internal crystalline water enables a faster conversion of CBD to the ordered ideal dolomite in a specific diagenetic environment. This study implies that the morphology and composition of microbial proto-dolomite may aid in identifying the type of dolomite-inducing microbes.