A novel tetraphenylbenzene-derived fluorescent probe for the detection of protamine and heparin

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI:10.1016/j.dyepig.2025.112656

Yutian Jiao, Yu Liu, Gongli Wei, Ce Wang, Baocai Xu, Li Zhao

引用次数: 0

Abstract

In this study, we present an amphiphilic aggregation-induced emission fluorescent probe based on tetraphenylbenzene. The probe exhibited sensitive and selective detection of protamine through a “turn-on” fluorescence signal driven by electrostatic binding. A linear correlation was established between fluorescence intensity and protamine concentration in the range of 0.8–3 μg/mL in 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid (HEPES) buffer, with a limit of detection (LOD) of 6.65 ng/mL. Protamine detection was successfully demonstrated in a 0.3 % serum sample. Additionally, the electrostatic probe–protamine complex was able to detect heparin owing to the strong affinity between protamine and heparin, which was confirmed through a “turn-off” fluorescence response. The probe's sensitivity and selectivity for heparin detection were validated, with an LOD of 8.83 ng/mL and a linear working range of 1.0–2.2 μg/mL in HEPES buffer.

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新型四苯基苯衍生荧光探针用于鱼精蛋白和肝素的检测

在这项研究中，我们提出了一种基于四苯基苯的两亲性聚集诱导发射荧光探针。该探针通过静电结合驱动的荧光信号显示出对鱼精蛋白的敏感和选择性检测。4-（2-羟乙基）哌嗪-1-乙烷磺酸（HEPES）缓冲液中鱼精蛋白浓度在0.8 ~ 3 μg/mL范围内与荧光强度呈线性相关，检出限（LOD）为6.65 ng/mL。在0.3%的血清样品中成功地证明了鱼精蛋白的检测。此外，由于鱼精蛋白和肝素之间具有很强的亲和力，静电探针-鱼精蛋白复合物能够检测肝素，这是通过“关闭”荧光响应证实的。在HEPES缓冲液中检测肝素的检出限为8.83 ng/mL，线性工作范围为1.0 ~ 2.2 μg/mL。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.