A novel tetraphenylbenzene-derived fluorescent probe for the detection of protamine and heparin

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-01-17 DOI:10.1016/j.dyepig.2025.112656

Yutian Jiao, Yu Liu, Gongli Wei, Ce Wang, Baocai Xu, Li Zhao

引用次数: 0

Abstract

In this study, we present an amphiphilic aggregation-induced emission fluorescent probe based on tetraphenylbenzene. The probe exhibited sensitive and selective detection of protamine through a “turn-on” fluorescence signal driven by electrostatic binding. A linear correlation was established between fluorescence intensity and protamine concentration in the range of 0.8–3 μg/mL in 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid (HEPES) buffer, with a limit of detection (LOD) of 6.65 ng/mL. Protamine detection was successfully demonstrated in a 0.3 % serum sample. Additionally, the electrostatic probe–protamine complex was able to detect heparin owing to the strong affinity between protamine and heparin, which was confirmed through a “turn-off” fluorescence response. The probe's sensitivity and selectivity for heparin detection were validated, with an LOD of 8.83 ng/mL and a linear working range of 1.0–2.2 μg/mL in HEPES buffer.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.