Efficient synthesis, purification and characterization of novel carboxymethyl cellulase from newly isolated Streptomyces sp. CSMPJR101, with potential use in denim biopolishing

Abstract

Microbial cellulases that are readily obtainable and grow on inexpensive substrates are the most economical. The study involved the isolation of 119 Streptomyces isolates that produced carboxymethyl cellulase (CMCase) from soils found in bamboo forests, Manjavadi Kanavai Tamilnadu, India. The isolate CSMPJR101 which produced highest zone of cleareance is used for the studies, upon optimization the isolate produced 111.8 ± 1 U/mL of crude CMCase. The cellulase produced by Streptomyces sp. CSMPJR101 had molecular mass of 86 kDa and biochemically characterized. The optimal enzyme activity found at pH 4–9 and temperature ranging from 30 to 50 °C. The CaCl₂ had a stimulatory effect on enzyme activity. The potential of the purified cellulase on biopolishing of denim farbrics was evaluated. The ability of Streptomyces-endoglucanase to lessen cloth hairiness, also increases fabric hydrophilicity for better moisture absorption and comfort. The novel cellulases produced from newly isolated Streptomyces sp. CSMPJR101 has a potential for textile processing.