Bioresource Technology Reports最新文献

Thermophilic and mesophilic two-phase co-digestion of the organic fraction of municipal solid waste with sewage sludge and equine manure 城市生活垃圾有机组分与污泥和马粪的嗜热和中温两相共消化

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-03 DOI: 10.1016/j.biteb.2026.102614

Laura André , Joel Awinzure Agumah , Flavien Picard , Xiaojun Liu , Thomas Moreau , Alain Magis , Olivier Bernat , Nabil Mabrouk , Florian Routhier , Antoine Brunet , Patrick Billette , André Pauss , Thierry Ribeiro

This study aimed to evaluate the feasibility of treating a unique mixture of substrates through two-phase anaerobic digestion at reduced retention times. Sewage sludge was co-digested with organic fraction of municipal solid waste and straw-rich equine manure in 20 L laboratory-scale anaerobic digesters operated under thermophilic and mesophilic conditions. Despite short hydraulic retention times (HRT) of 2 d in the thermophilic reactor followed by 12 or 15 d in the mesophilic reactor, no apparent process inhibition was observed. The system operated under high organic loading rates in the mesophilic phase (6.7 and 5.3 g_VS·L⁻¹·d⁻¹). Under these conditions, methane yields of approximately 235–245 NL_CH₄·kg_VS⁻¹ were achieved, corresponding to nearly 80% of the biochemical methane potential of the co-digested substrate (301 NL_CH₄·kg _VS⁻¹). The methanogenic reactor also showed good resilience, with gas production improving over the course of the experiment, with the volumetric methane production rate reaching 1.60 NL_CH₄·L⁻¹ reactor d⁻¹ at an HRT of 12 days and 1.27 NL_CH₄·L⁻¹ reactor·d⁻¹) at 15 days. The addition of strawed equine manure improved the C/N ratio and avoided potential inhibition by ammonia, reducing ammoniacal nitrogen concentrations from 4.7 g·L⁻¹ to 2.6 g·L⁻¹. The laboratory results indicated a strong potential for scale-up, because the system exhibited consistent performance and adaptability. These results were subsequently used in the design of a pilot plant processing 2–3 t·d⁻¹, which is currently operational.

{"title":"Thermophilic and mesophilic two-phase co-digestion of the organic fraction of municipal solid waste with sewage sludge and equine manure","authors":"Laura André , Joel Awinzure Agumah , Flavien Picard , Xiaojun Liu , Thomas Moreau , Alain Magis , Olivier Bernat , Nabil Mabrouk , Florian Routhier , Antoine Brunet , Patrick Billette , André Pauss , Thierry Ribeiro","doi":"10.1016/j.biteb.2026.102614","DOIUrl":"10.1016/j.biteb.2026.102614","url":null,"abstract":"<div><div>This study aimed to evaluate the feasibility of treating a unique mixture of substrates through two-phase anaerobic digestion at reduced retention times. Sewage sludge was co-digested with organic fraction of municipal solid waste and straw-rich equine manure in 20 L laboratory-scale anaerobic digesters operated under thermophilic and mesophilic conditions. Despite short hydraulic retention times (HRT) of 2 d in the thermophilic reactor followed by 12 or 15 d in the mesophilic reactor, no apparent process inhibition was observed. The system operated under high organic loading rates in the mesophilic phase (6.7 and 5.3 g<sub>VS</sub>·L<sup>−1</sup>·d<sup>−1</sup>). Under these conditions, methane yields of approximately 235–245 NL<sub>CH₄</sub>·kg<sub>VS</sub><sup>−1</sup> were achieved, corresponding to nearly 80% of the biochemical methane potential of the co-digested substrate (301 NL<sub>CH₄</sub>·kg <sub>VS</sub><sup>−1</sup>). The methanogenic reactor also showed good resilience, with gas production improving over the course of the experiment, with the volumetric methane production rate reaching 1.60 NL<sub>CH₄</sub>·L<sup>−1</sup> reactor d<sup>−1</sup> at an HRT of 12 days and 1.27 NL<sub>CH₄</sub>·L<sup>−1</sup> reactor·d<sup>−1</sup>) at 15 days. The addition of strawed equine manure improved the C/N ratio and avoided potential inhibition by ammonia, reducing ammoniacal nitrogen concentrations from 4.7 g·L<sup>−1</sup> to 2.6 g·L<sup>−1</sup>. The laboratory results indicated a strong potential for scale-up, because the system exhibited consistent performance and adaptability. These results were subsequently used in the design of a pilot plant processing 2–3 t·d<sup>−1</sup>, which is currently operational.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102614"},"PeriodicalIF":0.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and functional characterization of pyomelanin production by Shewanella indica biofilms on 3D-printed scaffolds for dye removal 在3d打印的染料去除支架上，印度希瓦氏菌生物膜产生脓黑素的基因组和功能表征

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102595

Jibin James , Silpa Chandran , Abdulaziz Anas , K. Devika Raj , Nizam Ashraf

Inspired by the interactions between sponges and bacteria within marine ecosystems, this study presents a scalable platform for the production of pyomelanin utilizing Shewanella indica immobilized on three-dimensional (3D) printed gyroid spheres with a surface area of 1835 mm². The application of five gyroid spheres immobilized with S. indica in 100 ml of growth medium resulted in a yield of 0.937 mg ml⁻¹ of pyomelanin, which could be enhanced to 3.76 mg ml⁻¹ when utilizing 20 spheres. The extraction of pyomelanin was accomplished through an alkali-acid treatment, and the gyroid spheres demonstrated reusability over five cycles. Furthermore, the pyomelanin exhibited an adsorption capacity of up to 70% for various textile dyes, including crystal violet and trypan blue, within a three-hour period. Genomic analysis identified genes associated with biofilm formation (lap A, B; lol A, B, D, E; DGC and bdl A) and pyomelanin biosynthesis (hppD, aspC, tyrA, and phhA). These results underscore a sustainable approach for pyomelanin production and its potential applications in bioremediation.

受海洋生态系统中海绵和细菌之间相互作用的启发，本研究提出了一个可扩展的平台，用于生产pyomelanin，利用固定化在三维（3D）打印的旋转球体上的印度希瓦氏菌（Shewanella indica），其表面积为1835 mm2。在100 ml培养基中使用5个固定化球，产黑素为0.937 mg ml−1，使用20个球时产黑素为3.76 mg ml−1。通过碱酸处理完成了黑素的提取，旋转球在5个循环中证明了可重复使用。此外，在3小时内，黑素对各种纺织染料（包括结晶紫和台锥蓝）的吸附量高达70%。基因组分析鉴定出与生物膜形成（lap A、B、lol A、B、D、E、DGC和bdl A）和黑素生物合成（hppD、aspC、tyrA和phhA）相关的基因。这些结果强调了一种可持续的产黑素方法及其在生物修复中的潜在应用。

{"title":"Genomic and functional characterization of pyomelanin production by Shewanella indica biofilms on 3D-printed scaffolds for dye removal","authors":"Jibin James , Silpa Chandran , Abdulaziz Anas , K. Devika Raj , Nizam Ashraf","doi":"10.1016/j.biteb.2026.102595","DOIUrl":"10.1016/j.biteb.2026.102595","url":null,"abstract":"<div><div>Inspired by the interactions between sponges and bacteria within marine ecosystems, this study presents a scalable platform for the production of pyomelanin utilizing <em>Shewanella indica</em> immobilized on three-dimensional (3D) printed gyroid spheres with a surface area of 1835 mm<sup>2</sup>. The application of five gyroid spheres immobilized with <em>S. indica</em> in 100 ml of growth medium resulted in a yield of 0.937 mg ml<sup>−1</sup> of pyomelanin, which could be enhanced to 3.76 mg ml<sup>−1</sup> when utilizing 20 spheres. The extraction of pyomelanin was accomplished through an alkali-acid treatment, and the gyroid spheres demonstrated reusability over five cycles. Furthermore, the pyomelanin exhibited an adsorption capacity of up to 70% for various textile dyes, including crystal violet and trypan blue, within a three-hour period. Genomic analysis identified genes associated with biofilm formation (lap A, B; lol A, B, D, E; DGC and bdl A) and pyomelanin biosynthesis (hppD, aspC, tyrA, and phhA). These results underscore a sustainable approach for pyomelanin production and its potential applications in bioremediation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102595"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penicillium oxalicum mediated phosphate solubilization as a Bio-stimulant approach enhancing barley growth 草青霉介导的磷酸盐增溶作为一种促进大麦生长的生物刺激剂

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102590

Hitakshi Asnani, Preeti Yadav, Yatika Dixit, Arun Kumar Sharma, Mansi Kansal

Phosphate-solubilizing microorganisms (PSMs) enhance plant phosphorus uptake by converting insoluble phosphorus into a soluble form. This study isolated and evaluated PSMs from barley rhizosphere soils in Tonk, Rajasthan, using Pikovskaya's agar medium. Six bacterial and six fungal strains demonstrated phosphate solubilization, with Penicillium oxalicum (isolate BLF-7) showing the highest efficiency. Penicillium oxalicum exhibited the highest phosphate solubilization index (1.9 ± 0.02) and efficiency (49.42% ± 0.99). Subsequent investigation indicated that P. oxalicum exhibited elevated concentrations of soluble phosphorus (1147.93 ± 2.43 mg/L) on the 10th day of incubation and exhibited acid phosphatase activity (13.18 ± 0.51 μM/min/mL) on the 11th day. Furthermore, it produced indole-3-acetic acid (3.36 μg/mL) within three days, facilitating plant development. Pot studies with barley demonstrated a 20% enhancement in plant height, notable improvements in root and shoot biomass, and a 20% rise in chlorophyll content in plants treated with P. oxalicum and tricalcium phosphate. These findings underscore P. oxalicum's dual role as a phosphate solubilizer and plant growth enhancer, affirming its promise as a sustainable biofertilizer.

增磷微生物（psm）通过将不溶性磷转化为可溶性磷来增强植物对磷的吸收。本研究利用Pikovskaya琼脂培养基从拉贾斯坦邦Tonk大麦根际土壤中分离和评价了psm。6株细菌和6株真菌表现出磷酸盐增溶作用，其中草酸青霉（BLF-7）表现出最高的增溶效果。草酸青霉的磷酸盐增溶指数最高（1.9±0.02），效率最高（49.42%±0.99）。随后的研究表明，草藻在培养第10天可溶性磷浓度升高（1147.93±2.43 mg/L），第11天酸性磷酸酶活性升高（13.18±0.51 μM/min/mL）。3 d内产生吲哚-3-乙酸（3.36 μg/mL），有利于植物发育。对大麦进行盆栽试验表明，草酸磷和磷酸三钙处理后，大麦株高增加20%，根和茎生物量显著增加，叶绿素含量增加20%。这些发现强调了草酸磷作为磷酸盐增溶剂和植物生长促进剂的双重作用，肯定了其作为可持续生物肥料的前景。

{"title":"Penicillium oxalicum mediated phosphate solubilization as a Bio-stimulant approach enhancing barley growth","authors":"Hitakshi Asnani, Preeti Yadav, Yatika Dixit, Arun Kumar Sharma, Mansi Kansal","doi":"10.1016/j.biteb.2026.102590","DOIUrl":"10.1016/j.biteb.2026.102590","url":null,"abstract":"<div><div>Phosphate-solubilizing microorganisms (PSMs) enhance plant phosphorus uptake by converting insoluble phosphorus into a soluble form. This study isolated and evaluated PSMs from barley rhizosphere soils in Tonk, Rajasthan, using Pikovskaya's agar medium. Six bacterial and six fungal strains demonstrated phosphate solubilization, with <em>Penicillium oxalicum</em> (isolate BLF-7) showing the highest efficiency. <em>Penicillium oxalicum</em> exhibited the highest phosphate solubilization index (1.9 ± 0.02) and efficiency (49.42% ± 0.99). Subsequent investigation indicated that <em>P. oxalicum</em> exhibited elevated concentrations of soluble phosphorus (1147.93 ± 2.43 mg/L) on the 10th day of incubation and exhibited acid phosphatase activity (13.18 ± 0.51 μM/min/mL) on the 11th day. Furthermore, it produced indole-3-acetic acid (3.36 μg/mL) within three days, facilitating plant development. Pot studies with barley demonstrated a 20% enhancement in plant height, notable improvements in root and shoot biomass, and a 20% rise in chlorophyll content in plants treated with <em>P. oxalicum</em> and tricalcium phosphate. These findings underscore <em>P. oxalicum</em>'s dual role as a phosphate solubilizer and plant growth enhancer, affirming its promise as a sustainable biofertilizer.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102590"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arsenic mitigation in spinach using Pseudomonas putida NBRI-RC5.2: Integrating functional traits with genomic insights 利用恶臭假单胞菌nbr - rc5.2减少菠菜中的砷：整合功能性状与基因组见解

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102586

Varsha Dharmesh , Nikita Tiwari , Satyam Rastogi , Ruchi Agnihotri , Prasanna Dutta , Sanjay Dwivedi , Meher Hasan Asif , Debasis Chakrabarty , Suchi Srivastava

Arsenic (As) mitigation recently gained prominence due to its escalating environmental risks. Vegetables, collected from affected areas, showed As accumulation ranging from 644 to 12,043 μg kg⁻¹. Among all tested vegetable crops, spinach showed the highest accumulation of As (874–12,043 μg kg⁻¹). Various arsenic-tolerant bacterial strains (∼763), along with plant growth-promoting traits, have been isolated from the rhizospheric and endophytic origins of these vegetables. Pseudomonas putida NBRI-RC5.2 (RC5.2), which possesses arsenate reductase and plant growth-promoting traits, was able to reduce the total As content in spinach leaves by ∼88%. Genes related to As resistance, plant growth promotion, and defense regulation have been identified through whole-genome analysis of RC5.2. The upregulated expression of arsR, arsB, arsA, arsK, arsJ, aqp, arsH and arsC genes in RC5.2 justifies its arsenic detoxification potential. The present study identified RC5.2 with a proven ability to tolerate and mitigate As uptake in spinach leaves. Development of RC5.2-based biofertilizer may be a promising candidate for sustainable As mitigation.

砷（As）缓解措施最近因其不断升级的环境风险而受到重视。从疫区收集的蔬菜显示砷积累量在644 ~ 12043 μg kg−1之间。在所有蔬菜作物中，菠菜的砷积累量最高（874 ~ 12043 μg kg−1）。从这些蔬菜的根际和内生起源中分离出多种具有促进植物生长特性的耐砷菌株（~ 763）。恶臭假单胞菌nbr -RC5.2 （RC5.2）具有砷酸盐还原酶和植物生长促进性状，能使菠菜叶片中总砷含量降低~ 88%。通过RC5.2的全基因组分析，已经鉴定出与抗砷、促进植物生长和防御调控相关的基因。在RC5.2中，arsR、arsB、arsA、arsK、arsJ、aqp、arsH和arsC基因的表达上调，证明了其对砷的解毒潜力。目前的研究发现RC5.2具有耐受和减轻菠菜叶片砷吸收的能力。基于rc5.2的生物肥料的开发可能是可持续缓解砷的有希望的候选物。

{"title":"Arsenic mitigation in spinach using Pseudomonas putida NBRI-RC5.2: Integrating functional traits with genomic insights","authors":"Varsha Dharmesh , Nikita Tiwari , Satyam Rastogi , Ruchi Agnihotri , Prasanna Dutta , Sanjay Dwivedi , Meher Hasan Asif , Debasis Chakrabarty , Suchi Srivastava","doi":"10.1016/j.biteb.2026.102586","DOIUrl":"10.1016/j.biteb.2026.102586","url":null,"abstract":"<div><div>Arsenic (As) mitigation recently gained prominence due to its escalating environmental risks. Vegetables, collected from affected areas, showed As accumulation ranging from 644 to 12,043 μg kg<sup>−1</sup>. Among all tested vegetable crops, spinach showed the highest accumulation of As (874–12,043 μg kg<sup>−1</sup>). Various arsenic-tolerant bacterial strains (∼763), along with plant growth-promoting traits, have been isolated from the rhizospheric and endophytic origins of these vegetables. <em>Pseudomonas putida</em> NBRI-RC5.2 (RC5.2), which possesses arsenate reductase and plant growth-promoting traits, was able to reduce the total As content in spinach leaves by ∼88%. Genes related to As resistance, plant growth promotion, and defense regulation have been identified through whole-genome analysis of RC5.2. The upregulated expression of <em>arsR</em>, <em>arsB</em>, <em>arsA</em>, <em>arsK</em>, <em>arsJ</em>, <em>aqp</em>, <em>arsH</em> and <em>arsC</em> genes in RC5.2 justifies its arsenic detoxification potential. The present study identified RC5.2 with a proven ability to tolerate and mitigate As uptake in spinach leaves. Development of RC5.2-based biofertilizer may be a promising candidate for sustainable As mitigation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102586"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed extract enhances biomass production and nutrient stress mitigation in fresh water microalga Scenedesmus sp. 海藻提取物提高淡水微藻的生物量生产和缓解营养胁迫。

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102581

Khushal Mehta , Shubham Rathod , Murali Krishna Paidi , Jayaseelan Murugaiyan , Imran Pancha

Microalgae are photosynthetic cell factories that can be used for the production of biofuel. However, lower biomass and lipid productivity are major limitations for their large-scale applications. The present study explores use of exogenous seaweed extract for enhancement of biomass and lipid content in microalgae. The addition of 1% v/v seaweed extract resulted in a biomass yield of 304 mg/L, which is higher than the BG-11 grown culture. Furthermore, it was observed that the addition of seaweed extract under nutrient stress conditions resulted in stress mitigation as well as enhancement in biomass production. Specifically, the addition of seaweed extract under nitrogen starvation resulted in nearly two-fold higher biomass with 43% lipid content. Similarly, seaweed extract addition under salinity stress conditions, resulted in approximately 1.8-fold higher biomass than the control culture. Further, metabolomics analysis revealed that the exogenous addition of seaweed extract enhances the concentration of key metabolites, thereby mitigating stress tolerance. This study highlights the potential application of exogenous seaweed extract as an economic and practical growth modulator for improving both biomass and biofuel production potential in microalgae.

微藻是可用于生产生物燃料的光合细胞工厂。然而，较低的生物量和脂质生产率是其大规模应用的主要限制。本研究探讨了外源海藻提取物对微藻生物量和脂质含量的提高。添加1% v/v海藻提取物，生物量产量为304 mg/L，高于BG-11培养物。此外，还观察到在营养胁迫条件下添加海藻提取物可以缓解胁迫并提高生物量产量。具体而言，在氮饥饿条件下添加海藻提取物可使生物量提高近两倍，脂质含量达到43%。同样，在盐度胁迫条件下添加海藻提取物，其生物量比对照培养高约1.8倍。此外，代谢组学分析显示，外源添加海藻提取物提高了关键代谢物的浓度，从而减轻了胁迫耐受性。本研究强调了外源海藻提取物作为一种经济实用的生长调节剂在提高微藻生物量和生物燃料生产潜力方面的潜在应用。

{"title":"Seaweed extract enhances biomass production and nutrient stress mitigation in fresh water microalga Scenedesmus sp.","authors":"Khushal Mehta , Shubham Rathod , Murali Krishna Paidi , Jayaseelan Murugaiyan , Imran Pancha","doi":"10.1016/j.biteb.2026.102581","DOIUrl":"10.1016/j.biteb.2026.102581","url":null,"abstract":"<div><div>Microalgae are photosynthetic cell factories that can be used for the production of biofuel. However, lower biomass and lipid productivity are major limitations for their large-scale applications. The present study explores use of exogenous seaweed extract for enhancement of biomass and lipid content in microalgae. The addition of 1% <em>v</em>/v seaweed extract resulted in a biomass yield of 304 mg/L, which is higher than the BG-11 grown culture. Furthermore, it was observed that the addition of seaweed extract under nutrient stress conditions resulted in stress mitigation as well as enhancement in biomass production. Specifically, the addition of seaweed extract under nitrogen starvation resulted in nearly two-fold higher biomass with 43% lipid content. Similarly, seaweed extract addition under salinity stress conditions, resulted in approximately 1.8-fold higher biomass than the control culture. Further, metabolomics analysis revealed that the exogenous addition of seaweed extract enhances the concentration of key metabolites, thereby mitigating stress tolerance. This study highlights the potential application of exogenous seaweed extract as an economic and practical growth modulator for improving both biomass and biofuel production potential in microalgae.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102581"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorizing soybean oil deodorizer distillate: Integrated membrane technology for squalane recovery via a cleaner production approach 大豆油脱臭馏出物的增值：通过清洁生产方法回收角鲨烷的集成膜技术

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102610

Maycon Morais Meira , Caroline Casagrande Sipoli , Rúbia Michele Suzuki , Aniely Cristina de Souza , Maria Carolina Sérgi Gomes

The growing demand for sustainable and natural ingredients in the cosmetic, pharmaceutical, and food industries has highlighted the potential of squalane, a high-value bioactive compound, particularly when derived from plant-based sources. However, current recovery methods from soybean oil deodorizer distillate (SODD) remain limited, as conventional techniques often rely on solvents, exhibit low selectivity, or require long processing times. Studies specifically addressing membrane-based valorization of SODD are still scarce. This study proposes an integrated and cleaner production approach for squalane recovery from SODD, a byproduct rich in unsaponifiable compounds. The methodology combines a saponification pretreatment with intermediate centrifugation and membrane microfiltration to enhance selectivity and mitigate membrane fouling. The results demonstrated that increasing the potassium hydroxide concentration to 20% during saponification significantly improved membrane selectivity. The integration of centrifugation before microfiltration further enhanced squalane recovery, yielding a squalane concentration in the permeate of 174.03 ± 2.43 mg 100 g sample⁻¹, which was approximately thirteen times higher compared to the retentate. This value represents the recovery yield achieved by the developed process. Therefore, the time required for squalane recovery in this study is about six times shorter, representing a significant advantage, even though the final concentration is lower. Overall, this membrane-based strategy proved to be an efficient, rapid, and solvent-free alternative for squalane recovery from SODD, promoting industrial byproduct valorization and aligning with circular economy principles and Sustainable Development Goal 12 on responsible production and consumption.

化妆品、制药和食品行业对可持续和天然成分的需求日益增长，这凸显了角鲨烷的潜力。角鲨烷是一种高价值的生物活性化合物，尤其是从植物来源提取的角鲨烷。然而，目前从大豆油脱臭馏出物（SODD）中回收的方法仍然有限，因为传统的技术通常依赖于溶剂，选择性低，或者需要较长的处理时间。专门针对SODD基于膜的增值的研究仍然很少。本研究提出了一种从富含不皂化化合物的副产品SODD中回收角鲨烷的综合清洁生产方法。该方法将皂化预处理与中间离心和膜微滤相结合，以提高选择性和减轻膜污染。结果表明，在皂化过程中，将氢氧化钾浓度提高到20%，可以显著提高膜的选择性。微滤前的离心整合进一步提高了角鲨烷的回收率，渗透液中角鲨烷的浓度为174.03±2.43 mg 100 g样品−1，比保留液高约13倍。该值表示开发的工艺所达到的回收率。因此，本研究中角鲨烷回收所需的时间缩短了约6倍，尽管最终浓度较低，但具有显著的优势。总的来说，这种基于膜的策略被证明是一种高效、快速、无溶剂的从SODD中回收角鲨烷的替代方法，促进了工业副产品的增值，符合循环经济原则和可持续发展目标12关于负责任的生产和消费。

{"title":"Valorizing soybean oil deodorizer distillate: Integrated membrane technology for squalane recovery via a cleaner production approach","authors":"Maycon Morais Meira , Caroline Casagrande Sipoli , Rúbia Michele Suzuki , Aniely Cristina de Souza , Maria Carolina Sérgi Gomes","doi":"10.1016/j.biteb.2026.102610","DOIUrl":"10.1016/j.biteb.2026.102610","url":null,"abstract":"<div><div>The growing demand for sustainable and natural ingredients in the cosmetic, pharmaceutical, and food industries has highlighted the potential of squalane, a high-value bioactive compound, particularly when derived from plant-based sources. However, current recovery methods from soybean oil deodorizer distillate (SODD) remain limited, as conventional techniques often rely on solvents, exhibit low selectivity, or require long processing times. Studies specifically addressing membrane-based valorization of SODD are still scarce. This study proposes an integrated and cleaner production approach for squalane recovery from SODD, a byproduct rich in unsaponifiable compounds. The methodology combines a saponification pretreatment with intermediate centrifugation and membrane microfiltration to enhance selectivity and mitigate membrane fouling. The results demonstrated that increasing the potassium hydroxide concentration to 20% during saponification significantly improved membrane selectivity. The integration of centrifugation before microfiltration further enhanced squalane recovery, yielding a squalane concentration in the permeate of 174.03 ± 2.43 mg 100 g sample<sup>−1</sup>, which was approximately thirteen times higher compared to the retentate. This value represents the recovery yield achieved by the developed process. Therefore, the time required for squalane recovery in this study is about six times shorter, representing a significant advantage, even though the final concentration is lower. Overall, this membrane-based strategy proved to be an efficient, rapid, and solvent-free alternative for squalane recovery from SODD, promoting industrial byproduct valorization and aligning with circular economy principles and Sustainable Development Goal 12 on responsible production and consumption.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102610"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of ammonia accumulation in anaerobic digestion of chicken manure by co-digestion and biogas-stripping 通过共消化和沼气剥离减少鸡粪厌氧消化过程中氨的积累

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102577

Sneha Prakash Naik , Vinoth Kumar Ponnusamy , Gopalakrishnan Kumar , Cing-Hong Yang , Pei-Chien Tsai , Chiu-Yue Lin , Gunda Mohanakrishna , Chyi-How Lay

Anaerobic digestion of chicken manure (CM) with high-solid content is typically constrained by ammonia nitrogen accumulation, which influences the microbial activity and methane yield (MY). A strategy of recycling stripped biogas and co-digestion was developed to reduce ammonia nitrogen accumulation and increase MY in a semi-continuously-stirred tank reactor (semi-CSTR) using CM as feedstock (total solid, 10%). The experimental results demonstrate that the stripping strategy can improve hydrolysis efficiency and reduce total ammonia nitrogen, free ammonia nitrogen, and total volatile fatty acids concentrations by 11%, 14%, and 20%, respectively. The soluble chemical oxygen demand (SCOD) removal efficiency and MY were improved by 23% and 37%, respectively compared to the no-stripping operation. In the integrated in-situ ammonia stripping with co-digestion study, with the addition of co-substrate (glucose, sunflower oil, and peptone), which was optimized by a central composite design (CCD) model, resulted in increased biogas yield and MY yield by 39% and 21% over the no-stripping method. The process also evidenced a lower TVFA concentration than the control, inferring the efficient conversion of metabolites towards methane production. This technology could be utilised in high-ammonia waste/wastewater digestion.

高固含量鸡粪的厌氧消化通常受到氨氮积累的限制，从而影响微生物活性和甲烷产量（MY）。在半连续搅拌槽式反应器（semi-CSTR）中，以CM为原料（总固体含量10%），开发了一种回收汽提沼气和共消化的策略，以减少氨氮的积累，提高MY。实验结果表明，溶出策略可以提高水解效率，使总氨氮、游离氨氮和总挥发性脂肪酸浓度分别降低11%、14%和20%。与无汽提操作相比，可溶化学需氧量（SCOD）去除率和MY去除率分别提高了23%和37%。在原位氨提与共消化的集成研究中，通过中心复合设计（CCD）模型优化的共底物（葡萄糖、葵花籽油和蛋白胨）的添加，使沼气产量和MY产量比不溶提法分别提高了39%和21%。该过程还证明了较低的TVFA浓度比对照，推断代谢产物有效转化为甲烷生产。该技术可用于高氨废水的消化。

{"title":"Reduction of ammonia accumulation in anaerobic digestion of chicken manure by co-digestion and biogas-stripping","authors":"Sneha Prakash Naik , Vinoth Kumar Ponnusamy , Gopalakrishnan Kumar , Cing-Hong Yang , Pei-Chien Tsai , Chiu-Yue Lin , Gunda Mohanakrishna , Chyi-How Lay","doi":"10.1016/j.biteb.2026.102577","DOIUrl":"10.1016/j.biteb.2026.102577","url":null,"abstract":"<div><div>Anaerobic digestion of chicken manure (CM) with high-solid content is typically constrained by ammonia nitrogen accumulation, which influences the microbial activity and methane yield (MY). A strategy of recycling stripped biogas and co-digestion was developed to reduce ammonia nitrogen accumulation and increase MY in a semi-continuously-stirred tank reactor (semi-CSTR) using CM as feedstock (total solid, 10%). The experimental results demonstrate that the stripping strategy can improve hydrolysis efficiency and reduce total ammonia nitrogen, free ammonia nitrogen, and total volatile fatty acids concentrations by 11%, 14%, and 20%, respectively. The soluble chemical oxygen demand (SCOD) removal efficiency and MY were improved by 23% and 37%, respectively compared to the no-stripping operation. In the integrated in-situ ammonia stripping with co-digestion study, with the addition of co-substrate (glucose, sunflower oil, and peptone), which was optimized by a central composite design (CCD) model, resulted in increased biogas yield and MY yield by 39% and 21% over the no-stripping method. The process also evidenced a lower TVFA concentration than the control, inferring the efficient conversion of metabolites towards methane production. This technology could be utilised in high-ammonia waste/wastewater digestion.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102577"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal dynamics of microplastic contamination in deep groundwater sources of urban drinking water supply in an arid region: Distribution, characterization, and risk index assessment 干旱区城市饮用水深层地下水中微塑料污染的季节性动态：分布、特征及风险指数评价

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102587

Sobhan Ghanbari , Farzaneh Mohammadi , Majid Hashemi , Hossein Movahedian Attar

This study offers an insightful investigation of microplastics (MPs) distribution and characterization in urban groundwater supply of Kerman arid region, Iran and provides new insights into the complex interaction of MPs properties, their seasonal dynamics, and the effect of local factors. MPs were detected in all samples during three seasons. The highest abundance of MPs was observed in spring (18.14 to 54.41 particles/L) followed by winter (18.14 to 39.29 particles/L) and autumn (9.07 to 30.23 particles/L). A spatial co-occurrence was observed between Shallower water levels and human activities such as cattle ranches, agriculture, mines, factories, and waste disposal as key factors in further contamination of qanats and aquifers 3 and 4. Fibers, black color, and small-sized 50 to 100 μm were the most abundant morphotype. Polypropylene and polyethylene were the most prevalent polymer types among seven types of identified polymers with the mean abundance percentages of 48.78% and 23.1%, respectively. The integrated polymer-based risk index assessment showed that the dominant pollution levels in autumn, winter, and spring were ranked at low, considerable, and considerable classes, respectively. Moreover, the relationship between MPs abundance and physicochemical properties of groundwater such as hardness, pH, EC, turbidity, and fecal coliforms was investigated to understand the transport and accumulation of MPs in groundwater systems. These findings extend beyond the urgent need for environmental monitoring in arid regions with groundwater supplies and provide an important basis and template for developing effective strategies to reduce MPs contamination in similar ecosystems globally.

本研究对伊朗克尔曼干旱区城市地下水中微塑料（MPs）的分布和特征进行了深入的研究，并对MPs特性、季节动态和局部因素影响的复杂相互作用提供了新的见解。在三个季节中，所有样品中都检测到MPs。MPs丰度以春季最高（18.14 ~ 54.41），其次为冬季（18.14 ~ 39.29）和秋季（9.07 ~ 30.23）。浅层水位与人类活动（如养牛场、农业、矿山、工厂和废物处理）之间存在空间共现关系，是造成坎儿井和含水层进一步污染的关键因素3和4。纤维、黑色、小尺寸50 ~ 100 μm是最丰富的形态。聚丙烯和聚乙烯是7种聚合物中最常见的聚合物类型，平均丰度百分比分别为48.78%和23.1%。基于聚合物的综合风险指数评价结果显示，秋季、冬季和春季的优势污染等级分别为低、相当和相当。此外，还研究了MPs丰度与地下水硬度、pH、EC、浊度和粪便大肠菌群等理化性质的关系，以了解MPs在地下水系统中的运移和积累。这些发现超出了对有地下水供应的干旱地区进行环境监测的迫切需要，并为制定有效的战略以减少全球类似生态系统中MPs污染提供了重要的基础和模板。

{"title":"Seasonal dynamics of microplastic contamination in deep groundwater sources of urban drinking water supply in an arid region: Distribution, characterization, and risk index assessment","authors":"Sobhan Ghanbari , Farzaneh Mohammadi , Majid Hashemi , Hossein Movahedian Attar","doi":"10.1016/j.biteb.2026.102587","DOIUrl":"10.1016/j.biteb.2026.102587","url":null,"abstract":"<div><div>This study offers an insightful investigation of microplastics (MPs) distribution and characterization in urban groundwater supply of Kerman arid region, Iran and provides new insights into the complex interaction of MPs properties, their seasonal dynamics, and the effect of local factors. MPs were detected in all samples during three seasons. The highest abundance of MPs was observed in spring (18.14 to 54.41 particles/L) followed by winter (18.14 to 39.29 particles/L) and autumn (9.07 to 30.23 particles/L). A spatial co-occurrence was observed between Shallower water levels and human activities such as cattle ranches, agriculture, mines, factories, and waste disposal as key factors in further contamination of qanats and aquifers 3 and 4. Fibers, black color, and small-sized 50 to 100 μm were the most abundant morphotype. Polypropylene and polyethylene were the most prevalent polymer types among seven types of identified polymers with the mean abundance percentages of 48.78% and 23.1%, respectively. The integrated polymer-based risk index assessment showed that the dominant pollution levels in autumn, winter, and spring were ranked at low, considerable, and considerable classes, respectively. Moreover, the relationship between MPs abundance and physicochemical properties of groundwater such as hardness, pH, EC, turbidity, and fecal coliforms was investigated to understand the transport and accumulation of MPs in groundwater systems. These findings extend beyond the urgent need for environmental monitoring in arid regions with groundwater supplies and provide an important basis and template for developing effective strategies to reduce MPs contamination in similar ecosystems globally.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102587"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose-response effects of microaeration on thermophilic lignocellulose-based anaerobic digestion: performance, stability and microbial community dynamics 微曝气对嗜热木质纤维素厌氧消化的剂量-响应效应：性能、稳定性和微生物群落动态

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102593

Marcin P. Kozera , Çağrı Akyol , Florent Bouchon , Ramon Ganigué , Bernard Willems , Erik Meers , Kai Bester , Henrik B. Møller

Microaeration has emerged as a promising strategy for enhancing anaerobic digestion (AD) of lignocellulosic biomass, although inconsistent results highlight the need for further study. This study tested three different aeration rates (1, 5 and 8 mLO₂/gVS) on anaerobic co-digestion of wheat straw and cattle manure under thermophilic conditions (55 °C) in continuous-stirred tank reactors with working volume of 60 L. Daily injection of 5 mLO₂/gVS achieved the highest methane yield, with a 20.6% increase compared to the non-aerated control digester. Under these conditions, cellulose and hemicellulose degradation reached maximum levels of 75% and 66.3%, respectively. In the microaerated digester, remarkable changes in microbial population were observed. Injection of 1 mLO₂/gVS and 5 mLO₂/gVS increased the relative abundance of Lentimicrobium, Turicibacter, and unclassified MBA03 as well as hydrogenotrophic Methanothermobacter which could contribute to enhanced lignocellulose degradation and biogas generation. At the final and highest oxygen dose (8 mLO₂/gVS), methane yield began to gradually decrease. Under these conditions, the microbial profile shifted back to that of the non-aerated digester, suggesting that the activity of certain microbial groups was inhibited. These findings highlight the potential of controlled microaeration to enhance both methane yield and lignocellulosic degradation at larger scales, offering valuable insights for optimizing AD processes.

微曝气已成为加强木质纤维素生物质厌氧消化（AD）的一种有前途的策略，尽管不一致的结果表明需要进一步研究。本研究在连续搅拌槽式反应器中测试了3种不同曝气率（1、5和8 mLO2/gVS）在耐热条件下（55°C）在工作体积为60 l的连续搅拌槽式反应器中对麦秸和牛粪厌氧共消化的效果，日曝气量为5 mLO2/gVS时甲烷产量最高，比不曝气的对照沼气池增加20.6%。在此条件下，纤维素和半纤维素的最高降解率分别达到75%和66.3%。在微曝气消化池中，观察到微生物种群的显著变化。注射1 mLO2/gVS和5 mLO2/gVS增加了lentimicroum、Turicibacter和未分类MBA03以及氢营养甲烷热杆菌的相对丰度，有助于增强木质纤维素的降解和沼气的产生。在最终和最高氧剂量（8 mLO2/gVS）时，甲烷产率开始逐渐下降。在这些条件下，微生物分布恢复到未曝气沼气池的状态，表明某些微生物群的活性受到抑制。这些发现强调了控制微曝气在更大规模上提高甲烷产量和木质纤维素降解的潜力，为优化AD工艺提供了有价值的见解。

{"title":"Dose-response effects of microaeration on thermophilic lignocellulose-based anaerobic digestion: performance, stability and microbial community dynamics","authors":"Marcin P. Kozera , Çağrı Akyol , Florent Bouchon , Ramon Ganigué , Bernard Willems , Erik Meers , Kai Bester , Henrik B. Møller","doi":"10.1016/j.biteb.2026.102593","DOIUrl":"10.1016/j.biteb.2026.102593","url":null,"abstract":"<div><div>Microaeration has emerged as a promising strategy for enhancing anaerobic digestion (AD) of lignocellulosic biomass, although inconsistent results highlight the need for further study. This study tested three different aeration rates (1, 5 and 8 mLO<sub>2</sub>/gVS) on anaerobic co-digestion of wheat straw and cattle manure under thermophilic conditions (55 °C) in continuous-stirred tank reactors with working volume of 60 L. Daily injection of 5 mLO<sub>2</sub>/gVS achieved the highest methane yield, with a 20.6% increase compared to the non-aerated control digester. Under these conditions, cellulose and hemicellulose degradation reached maximum levels of 75% and 66.3%, respectively. In the microaerated digester, remarkable changes in microbial population were observed. Injection of 1 mLO<sub>2</sub>/gVS and 5 mLO<sub>2</sub>/gVS increased the relative abundance of <em>Lentimicrobium</em>, <em>Turicibacter</em>, and <em>unclassified MBA03</em> as well as hydrogenotrophic <em>Methanothermobacter</em> which could contribute to enhanced lignocellulose degradation and biogas generation. At the final and highest oxygen dose (8 mLO<sub>2</sub>/gVS), methane yield began to gradually decrease. Under these conditions, the microbial profile shifted back to that of the non-aerated digester, suggesting that the activity of certain microbial groups was inhibited. These findings highlight the potential of controlled microaeration to enhance both methane yield and lignocellulosic degradation at larger scales, offering valuable insights for optimizing AD processes.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102593"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable nutrient recovery process in brackish aquaculture effluent with microalgae technology 微藻技术在微咸养殖废水中可持续养分回收的研究

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-02-01 DOI: 10.1016/j.biteb.2026.102611

Julia Monje Díaz , Jesús Ruiz , José Antonio Perales Vargas-Machuca , Carmen Garrido Pérez , María Dolores Macías-Sánchez

The rapid expansion of the aquaculture industry has led to the generation of large volumes of nutrient-rich wastewater, posing significant environmental risks if not properly managed. This study investigates Parachlorella kessleri for treating brackish effluent within a ‘Circular Economy’ framework. Cultivation was tested in 2 L batch and 8 L semi-continuous reactors under various nutrient supplementations. Maximum batch productivity (91.72 mgSS·L⁻¹·d⁻¹) required phosphorus and micronutrients addition, achieving nutrient removal below the permitted limit. In semi-continuous operation productivity reached 50.55 mgSS·L⁻¹·d⁻¹. Results indicate that unsupplemented batch operation maximises lipid content, while supplemented semi-continuous operation favors carbohydrate and protein enrichment. These findings demonstrate a scalable pathway for integrating microalgae into industrial aquaculture, providing reclaimed water for reuse and high-value biomass for sustainable feed or biofuels. This approach closes the nutrient loop, enhancing resource efficiency in the industry.

水产养殖业的迅速扩张导致产生大量富含营养的废水，如果管理不当，将构成重大的环境风险。本研究调查了在“循环经济”框架下处理微咸废水的凯斯利伞藻。在2 L间歇反应器和8 L半连续反应器中进行了不同营养补充条件下的培养试验。最大批生产率（91.72 mgSS·L−1·d−1）需要添加磷和微量营养素，以实现低于允许限度的营养去除。半连续运行时，生产率达到50.55 mgSS·L−1·d−1。结果表明，未添加间歇操作可使脂肪含量最大化，而添加半连续操作有利于碳水化合物和蛋白质的富集。这些发现证明了将微藻整合到工业水产养殖、提供可重复使用的再生水和高价值生物质用于可持续饲料或生物燃料的可扩展途径。这种方法关闭了营养循环，提高了行业的资源效率。

{"title":"Sustainable nutrient recovery process in brackish aquaculture effluent with microalgae technology","authors":"Julia Monje Díaz , Jesús Ruiz , José Antonio Perales Vargas-Machuca , Carmen Garrido Pérez , María Dolores Macías-Sánchez","doi":"10.1016/j.biteb.2026.102611","DOIUrl":"10.1016/j.biteb.2026.102611","url":null,"abstract":"<div><div>The rapid expansion of the aquaculture industry has led to the generation of large volumes of nutrient-rich wastewater, posing significant environmental risks if not properly managed. This study investigates <em>Parachlorella kessleri</em> for treating brackish effluent within a ‘Circular Economy’ framework. Cultivation was tested in 2 L batch and 8 L semi-continuous reactors under various nutrient supplementations. Maximum batch productivity (91.72 mgSS·L<sup>−1</sup>·d<sup>−1</sup>) required phosphorus and micronutrients addition, achieving nutrient removal below the permitted limit. In semi-continuous operation productivity reached 50.55 mgSS·L<sup>−1</sup>·d<sup>−1</sup>. Results indicate that unsupplemented batch operation maximises lipid content, while supplemented semi-continuous operation favors carbohydrate and protein enrichment. These findings demonstrate a scalable pathway for integrating microalgae into industrial aquaculture, providing reclaimed water for reuse and high-value biomass for sustainable feed or biofuels. This approach closes the nutrient loop, enhancing resource efficiency in the industry.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102611"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0