From waste to fuel: Harnessing high specificity lipases from Candida rugosa fermentation for sustainable biodiesel

Q1 Environmental Science Bioresource Technology Reports Pub Date : 2025-02-01 Epub Date: 2025-01-19 DOI:10.1016/j.biteb.2025.102039

Anil Kumar, Gursimar Singh, Karanvir Singh, Vishwender Pratap Singh, Aradhana Srivastava, Arinjay Kumar

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Abstract

Present study focuses on lipases from Candida rugosa fermentation using two carbon substrates (glucose and maltose) in synthetic medium and their characterization with activities against known standards, and additional application in biodiesel production from rubber seed oil to show their substrate specificity in transesterification. Synthetic medium with maltose and glucose produces the maximum lipase activities of 50,400 U/L and 11,520 U/L, respectively. The lipases loaded on SDS-PAGE are characterized with sizes 57 and 61 kDa. Specificity of produced lipase is tested, inedible rubber seed oil is biotransesterified, which exhibits high lipase specificity as acting on the long chain fatty acids (up to C₂₅) to yield 94.6 % biodiesel. This study confirms the high potential of lipases in serving United Nations Sustainable Development Goal 7 (clean energy). Lipases transesterify saturated and unsaturated fatty acids and produce quality biodiesel assessed by its density-872 kg/m³, kinematic viscosity-5.344 cS, and calorific value-9988 kcal/kg.

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从废物到燃料：利用高特异性脂肪酶从念珠菌发酵可持续生物柴油

目前的研究重点是在合成培养基中使用两种碳底物（葡萄糖和麦芽糖）发酵的念珠菌脂肪酶，以及它们在已知标准下的活性表征，以及在橡胶籽油生产生物柴油中的应用，以显示它们在酯交换过程中的底物特异性。麦芽糖和葡萄糖合成培养基的脂肪酶活性最高，分别为50,400 U/L和11,520 U/L。SDS-PAGE上负载的脂肪酶大小分别为57和61 kDa。对所得脂肪酶的特异性进行了测试，对不可食用橡胶籽油进行了生物酯交换，对长链脂肪酸（高达C25）起作用，表现出较高的脂肪酶特异性，产率为94.6%。这项研究证实了脂肪酶在服务于联合国可持续发展目标7（清洁能源）方面的巨大潜力。脂肪酶能对饱和和不饱和脂肪酸进行酯交换，生产出高质量的生物柴油，其密度为872 kg/m3，运动粘度为5.344 cS，热值为9988 kcal/kg。

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