From waste to fuel: Harnessing high specificity lipases from Candida rugosa fermentation for sustainable biodiesel

Q1 Environmental Science Bioresource Technology Reports Pub Date : 2025-02-01 DOI:10.1016/j.biteb.2025.102039

Anil Kumar, Gursimar Singh, Karanvir Singh, Vishwender Pratap Singh, Aradhana Srivastava, Arinjay Kumar

{"title":"From waste to fuel: Harnessing high specificity lipases from Candida rugosa fermentation for sustainable biodiesel","authors":"Anil Kumar, Gursimar Singh, Karanvir Singh, Vishwender Pratap Singh, Aradhana Srivastava, Arinjay Kumar","doi":"10.1016/j.biteb.2025.102039","DOIUrl":null,"url":null,"abstract":"<div><div>Present study focuses on lipases from <em>Candida rugosa</em> fermentation using two carbon substrates (glucose and maltose) in synthetic medium and their characterization with activities against known standards, and additional application in biodiesel production from rubber seed oil to show their substrate specificity in transesterification. Synthetic medium with maltose and glucose produces the maximum lipase activities of 50,400 U/L and 11,520 U/L, respectively<strong>.</strong> The lipases loaded on SDS-PAGE are characterized with sizes 57 and 61 kDa. Specificity of produced lipase is tested, inedible rubber seed oil is biotransesterified, which exhibits high lipase specificity as acting on the long chain fatty acids (up to C<sub>25</sub>) to yield 94.6 % biodiesel. This study confirms the high potential of lipases in serving United Nations Sustainable Development Goal 7 (clean energy). Lipases transesterify saturated and unsaturated fatty acids and produce quality biodiesel assessed by its density-872 kg/m<sup>3</sup>, kinematic viscosity-5.344 cS, and calorific value-9988 kcal/kg.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102039"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589014X25000210","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 0

Abstract

Present study focuses on lipases from Candida rugosa fermentation using two carbon substrates (glucose and maltose) in synthetic medium and their characterization with activities against known standards, and additional application in biodiesel production from rubber seed oil to show their substrate specificity in transesterification. Synthetic medium with maltose and glucose produces the maximum lipase activities of 50,400 U/L and 11,520 U/L, respectively. The lipases loaded on SDS-PAGE are characterized with sizes 57 and 61 kDa. Specificity of produced lipase is tested, inedible rubber seed oil is biotransesterified, which exhibits high lipase specificity as acting on the long chain fatty acids (up to C₂₅) to yield 94.6 % biodiesel. This study confirms the high potential of lipases in serving United Nations Sustainable Development Goal 7 (clean energy). Lipases transesterify saturated and unsaturated fatty acids and produce quality biodiesel assessed by its density-872 kg/m³, kinematic viscosity-5.344 cS, and calorific value-9988 kcal/kg.

Abstract Image