Food authentication using the loop-mediated isothermal amplification (LAMP) method: Current trends and future prospects

Abstract

Background

The rapid globalization of food supply chain has significantly increased risk of food fraud, including the misrepresentation of product origins, adulteration, and the use of unauthorized additives. Consequently, the need for robust and reliable food authentication methods has become paramount. Loop-mediated isothermal amplification (LAMP) has emerged as an alternative to conventional polymerase chain reaction, offering unique advantages for tackling the complexities of food authentication. Its unique features, including isothermal operation, high specificity, and rapid nucleic acid amplification make it particularly effective for detecting fraudulent practices involving different biological species.

Scope and approach

This article reviews the application of the LAMP method in food authentication, focusing on its versatility across various food matrices, fraud practices, and target analytes. It highlights recent advancements, innovative adaptations, and the integration of LAMP with other technologies (e.g., lateral flow assay) to enhance both efficiency and accuracy.

Key findings and conclusions

This review highlights the effectiveness of the LAMP method in detecting food fraud and adulteration, emphasizing its suitability for on-site testing and potential to revolutionize routine food quality control. As the food industry strives for transparency and authenticity, LAMP stands out as a pivotal tool for ensuring the integrity of the global food supply.