A comprehensive review of Interferometric Reflectance Imaging Sensor as a sensitive detection platform and its application areas

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology Biosensors and Bioelectronics: X Pub Date : 2025-01-07 DOI:10.1016/j.biosx.2025.100574

Monireh Bakhshpour-Yucel , Nese Lortlar Unlu , Elif Seymour , Adil Denizli

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Abstract

The Interferometric Reflectance Imaging Sensor (IRIS) technology represents a significant advancement in biosensing, providing a label-free, selective, sensitive, and high-throughput platform for detecting molecular interactions. This review explores the underlying principles, instrumentation, and diverse applications of IRIS, with a focus on its efficacy for real-time monitoring of DNA-protein and protein-protein interactions, as well as virus detection. IRIS can measure DNA hybridization kinetics and identify pathogens without labeling, highlighting its versatility and reliability in biomedical research and diagnostics. IRIS achieves enhanced sensitivity and specificity by leveraging spectral reflectivity as a transduction mechanism and employing a 3D polymeric surface chemistry for bioreceptor immobilization. The review underscores IRIS's potential to revolutionize clinical diagnostics, biomolecular screening, and the study of biomolecular binding affinities, establishing it as a powerful tool for future research and medical applications.

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综述了干涉反射成像传感器作为一种灵敏的检测平台及其应用领域

干涉反射成像传感器（IRIS）技术代表了生物传感领域的重大进步，为检测分子相互作用提供了无标记、选择性、灵敏度和高通量的平台。本文综述了IRIS的基本原理、仪器和各种应用，重点介绍了IRIS在实时监测dna -蛋白质和蛋白质-蛋白质相互作用以及病毒检测方面的功效。IRIS可以测量DNA杂交动力学和识别病原体而无需标记，突出了其在生物医学研究和诊断中的多功能性和可靠性。IRIS通过利用光谱反射率作为转导机制，并采用3D聚合物表面化学进行生物受体固定化，从而提高了灵敏度和特异性。该综述强调了IRIS在临床诊断、生物分子筛选和生物分子结合亲和力研究方面的革命性潜力，并将其确立为未来研究和医学应用的有力工具。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.