Henrique Fonteles , T.S. Klippel , Daphne Tórgo , Felipe F. Selau , Bárbara Konrad , Daniel L. Baptista , Jonder Morais , Maria do Carmo Martins Alves , Guido Lenz , Johnny F. Dias , Pedro L. Grande
{"title":"Cellular uptake of gold nanoparticles in glioblastoma cells revealed by μ-PIXE","authors":"Henrique Fonteles , T.S. Klippel , Daphne Tórgo , Felipe F. Selau , Bárbara Konrad , Daniel L. Baptista , Jonder Morais , Maria do Carmo Martins Alves , Guido Lenz , Johnny F. Dias , Pedro L. Grande","doi":"10.1016/j.nimb.2024.165607","DOIUrl":null,"url":null,"abstract":"<div><div>The use of NPs has increased massively in numerous fields, including environmental sciences, electronics, and medicine. Because of their unique physical, optical, and biological capabilities, gold nanoparticles (AuNPs) are of considerable interest. These nanoparticles have distinct properties that make them useful as nanoprobes for imaging and nanocarriers for efficient drug delivery systems, for example. To harness their full potential in the biomedical area, it is crucial to accurately characterize their size, shape, and biological activity. In this study, the <span><math><mi>μ</mi></math></span>-PIXE technique has been employed to investigate the cellular uptake of these nanoparticles upon interaction with the U87 glioblastoma cell line. This analysis provided information on the internalization and distribution of nanoparticles within the cellular environment. To evaluate the cytotoxic effects of the AuNPs, the MTT assay was performed. This widely used method allows for the assessment of cell viability in the presence of nanoparticles. Lastly, their size was also measured by scanning electron microscopy, yielding a mean diameter of 18 ± 5 nm, which agreed well with previous MEIS results.</div></div>","PeriodicalId":19380,"journal":{"name":"Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms","volume":"560 ","pages":"Article 165607"},"PeriodicalIF":1.4000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms","FirstCategoryId":"101","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168583X2400377X","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
引用次数: 0
Abstract
The use of NPs has increased massively in numerous fields, including environmental sciences, electronics, and medicine. Because of their unique physical, optical, and biological capabilities, gold nanoparticles (AuNPs) are of considerable interest. These nanoparticles have distinct properties that make them useful as nanoprobes for imaging and nanocarriers for efficient drug delivery systems, for example. To harness their full potential in the biomedical area, it is crucial to accurately characterize their size, shape, and biological activity. In this study, the -PIXE technique has been employed to investigate the cellular uptake of these nanoparticles upon interaction with the U87 glioblastoma cell line. This analysis provided information on the internalization and distribution of nanoparticles within the cellular environment. To evaluate the cytotoxic effects of the AuNPs, the MTT assay was performed. This widely used method allows for the assessment of cell viability in the presence of nanoparticles. Lastly, their size was also measured by scanning electron microscopy, yielding a mean diameter of 18 ± 5 nm, which agreed well with previous MEIS results.
期刊介绍：
Section B of Nuclear Instruments and Methods in Physics Research covers all aspects of the interaction of energetic beams with atoms, molecules and aggregate forms of matter. This includes ion beam analysis and ion beam modification of materials as well as basic data of importance for these studies. Topics of general interest include: atomic collisions in solids, particle channelling, all aspects of collision cascades, the modification of materials by energetic beams, ion implantation, irradiation - induced changes in materials, the physics and chemistry of beam interactions and the analysis of materials by all forms of energetic radiation. Modification by ion, laser and electron beams for the study of electronic materials, metals, ceramics, insulators, polymers and other important and new materials systems are included. Related studies, such as the application of ion beam analysis to biological, archaeological and geological samples as well as applications to solve problems in planetary science are also welcome. Energetic beams of interest include atomic and molecular ions, neutrons, positrons and muons, plasmas directed at surfaces, electron and photon beams, including laser treated surfaces and studies of solids by photon radiation from rotating anodes, synchrotrons, etc. In addition, the interaction between various forms of radiation and radiation-induced deposition processes are relevant.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
