Rational design of fast-responding and highly selective fluorescent probes for detecting and imaging acetylcholinesterase homeostatic changes in Alzheimer's disease

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-01-15 DOI:10.1016/j.dyepig.2025.112636

Inam Ullah , Chenrun Zhang , Yiwen Shao, Jiawen Shen, Hai-Liang Zhu

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Abstract

Alzheimer's disease (AD) is a progressive amnestic cognitive deteriorating neurodegenerative disease. Acetylcholinesterase (AChE) has a very high catalytic hydrolysis efficiency, which can rapidly hydrolyze acetylcholine into choline, playing a critical role in regulating the nervous system. Therefore, we designed and synthesized a fluorescent probe capable of quantitatively detecting AChE called ACP. Most strikingly, an extremely low detection limit of 0.341U/mL is far superior to those of many probes. The approach of utilizing ACP enabled us to swiftly identify and track real-time alterations in intracellular AChE levels. Additionally, our study marks the pioneering use of ACP in effectively detecting an increase in AChE expression in C. elegans and mice models of Alzheimer's disease (AD). In conclusion, this study provides a new tool for quantitatively detecting AChE levels and shows great potential for application in AChE-related studies.

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合理设计快速反应、高选择性的检测和成像阿尔茨海默病乙酰胆碱酯酶稳态变化的荧光探针

阿尔茨海默病（AD）是一种进行性遗忘性认知退化性神经退行性疾病。乙酰胆碱酯酶（AChE）具有非常高的催化水解效率，能将乙酰胆碱快速水解为胆碱，在调节神经系统中起着至关重要的作用。因此，我们设计并合成了一种能够定量检测AChE的荧光探针ACP。最引人注目的是，极低的检测限为0.341U/mL，远远优于许多探针。利用ACP的方法使我们能够快速识别和跟踪细胞内AChE水平的实时变化。此外，我们的研究标志着ACP在线虫和阿尔茨海默病（AD）小鼠模型中有效检测AChE表达增加的开创性应用。总之，本研究为AChE的定量检测提供了新的工具，在AChE相关研究中具有很大的应用潜力。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.