Ying Zhou , Wenlong Wang , Wanping Zhu , Tingting Cai , Nannan Wang , Xia Liu , Wenmin Wang , Kequn Chai
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引用次数: 0
Abstract
Background
Pulmonary fibrosis (PF) is a chronic interstitial lung disease posing significant health risks. This study aimed to investigate the therapeutic mechanism of Qingfei Tongluo mixture (QTm) in treating PF by combining network pharmacology and experimental verification.
Results
A total of 246 active ingredients in QTm were identified, with 159 potential targets for PF treatment. Quercetin, a key active ingredient, was associated with the TGF-β1 signaling pathway. Gene Ontology and KEGG enrichment analyses identified 42 core genes, with a notable implication of the TGF-beta signaling pathway in PF. Immunohistochemistry showed elevated FTO and TGF-β1 levels in PF tissues. Animal experiments demonstrated that QTm improved alveolar structure, reduced interstitial lesions, and enhanced lung function while decreasing hydroxyproline content and the expression of FTO and TGF-β1 proteins.
Conclusions
QTm may inhibit PF progression by suppressing FTO/TGF-β1 expression, thereby improving lung function. These findings suggest that QTm holds potential as a treatment for PF.
How to cite: Zhou Y, Wang W, Zhu W, et al. Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.10.002.
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
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• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
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• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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