Electronic Journal of Biotechnology最新文献

{"title":"Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress","authors":"Yaqian Liu,&nbsp;Yonggang Sun,&nbsp;Yincang Han,&nbsp;Weiqin Ding,&nbsp;Shengwei Jin,&nbsp;Jianyu Chen,&nbsp;Fajie Gou","doi":"10.1016/j.ejbt.2024.10.004","DOIUrl":"10.1016/j.ejbt.2024.10.004","url":null,"abstract":"<div><h3>Background</h3><div>The aim of this study was to screen circRNAs related to fat deposition in yaks, and to identify candidate circRNAs for yak meat quality improvement. Six male yaks with insignificant differences in body weights were selected as test subjects, and 3 yaks (G18_IMF) were randomly slaughtered at the beginning of the experiment, while the remaining 3 yaks were naturally grazed until 24 months of age (G24_IMF), and then slaughtered at the end of the experiment, and the intramuscular fat was collected from the dorsal muscle. At the end of the experiment, the yaks were slaughtered and the intramuscular fat from the back was collected for whole transcriptome sequencing.</div></div><div><h3>Results</h3><div>The results showed that 352 differential circRNAs, 86 differential miRNAs and 3981 differential mRNAs were found. miRNAs and mRNAs network regulation maps were successfully constructed through gene expression correlation analysis and target gene prediction.</div></div><div><h3>Conclusions</h3><div>Taking the intersection of the predicted circRNA target genes with the differential miRNAs for intramuscular fat in yaks of different months of age, we obtained two candidate ceRNA pairs that might be related to intramuscular fat deposition in yaks, and found that bta-let-7i might be related to fat deposition in yaks and might be regulated by X_85287959_85291068, and that X_85287959_85291068 could be a candidate circRNA to enhance the quality of yak meat. The results may provide a reference for further investigation of the regulatory network of intramuscular fat deposition in yak.</div><div><strong>How to cite:</strong> Liu Y, Sun Y, Han Y, et al. Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 11-18"},"PeriodicalIF":2.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143284229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis","authors":"Ying Zhou ,&nbsp;Wenlong Wang ,&nbsp;Wanping Zhu ,&nbsp;Tingting Cai ,&nbsp;Nannan Wang ,&nbsp;Xia Liu ,&nbsp;Wenmin Wang ,&nbsp;Kequn Chai","doi":"10.1016/j.ejbt.2024.10.002","DOIUrl":"10.1016/j.ejbt.2024.10.002","url":null,"abstract":"<div><h3>Background</h3><div>Pulmonary fibrosis (PF) is a chronic interstitial lung disease posing significant health risks. This study aimed to investigate the therapeutic mechanism of Qingfei Tongluo mixture (QTm) in treating PF by combining network pharmacology and experimental verification.</div></div><div><h3>Results</h3><div>A total of 246 active ingredients in QTm were identified, with 159 potential targets for PF treatment. Quercetin, a key active ingredient, was associated with the TGF-β1 signaling pathway. Gene Ontology and KEGG enrichment analyses identified 42 core genes, with a notable implication of the TGF-beta signaling pathway in PF. Immunohistochemistry showed elevated FTO and TGF-β1 levels in PF tissues. Animal experiments demonstrated that QTm improved alveolar structure, reduced interstitial lesions, and enhanced lung function while decreasing hydroxyproline content and the expression of FTO and TGF-β1 proteins.</div></div><div><h3>Conclusions</h3><div>QTm may inhibit PF progression by suppressing FTO/TGF-β1 expression, thereby improving lung function. These findings suggest that QTm holds potential as a treatment for PF.</div><div><strong>How to cite:</strong> Zhou Y, Wang W, Zhu W, et al. Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 38-47"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by Penicillium lanosocoeruleum KSA-55","authors":"Eman Alhomaidi","doi":"10.1016/j.ejbt.2024.10.003","DOIUrl":"10.1016/j.ejbt.2024.10.003","url":null,"abstract":"<div><h3>Background</h3><div>The synthesis of keratinases by psychrophilic and psychrotolerant microorganisms has not received much attention, despite the fact that they can be an effective stand-in for substrate conversion at a low energy cost.</div></div><div><h3>Results</h3><div>In this study, some psychrophilic fungi isolated from three cold storage locations near Riyadh, Saudi Arabia, were tested for their cold-active keratinase potential. <em>Penicillium lanosocoeruleum</em>, a recently isolated fungus from Saudi Arabia, was the potent strain that produced cold-active keratinase. The <em>Penicillium</em> species was identified using sequencing of the internal transcribed spacer region (ITS). The generation of cold-active keratinase by <em>P. lanosocoeruleum</em> KSA-55 was optimized by two factors at time (TFAT). At pH 9.0 and 15°C, the keratinase activity was 28.9 ± 2.8 U/mL/min which increased to 41.7 ± 3.8 U/mL/min after 6 d of fermentation using peptone as a nitrogen source. The produced keratinase was chromatographed by MP 800 anion exchange resin and Sephadex G 100 size exclusion gel. At pH 9.0 and 10°C, the pure keratinase displayed the maximum specific activity of 210.3 ± 8.4 U/mg. Zn²⁺, Fe²⁺, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and phenylmethanesulfonyl fluoride (PMSF), demonstrated severe inhibitory effects on the keratinase activity. Mn²⁺ ions activated the keratinase by 166.85 ± 15.6%. PMSF significantly reduced keratinase activity.</div></div><div><h3>Conclusions</h3><div><em>P. lanosocoeruleum</em> strain KSA-55 is presented here as a new prospective producer of cold-active keratinase for a variety of biotechnological uses, including the management of keratinous waste in the poultry industry, cosmetics, and medical applications.</div><div><strong>How to cite:</strong> Alhomaidi E. A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by <em>Penicillium lanosocoeruleum</em> KSA-55. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 1-10"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143169491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry","authors":"Oliver Birrenbach ,&nbsp;Peter Czermak","doi":"10.1016/j.ejbt.2024.10.001","DOIUrl":"10.1016/j.ejbt.2024.10.001","url":null,"abstract":"<div><h3>Background</h3><div>The efficiency of recombinant protein production in <em>Yarrowia lipolytica</em> is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the <em>Y. lipolytica</em> auxotrophic strain PO1f.</div></div><div><h3>Results</h3><div>Statistically supported optimal concentrations of 10 g*L⁻¹ glucose and 2.29 g*L⁻¹ leucine enabled superior growth in the new <em>in silico</em> yeast (ISY) medium. Specific growth rates of 0.305 h⁻¹ were achieved for the auxotrophic <em>Yarrowia</em> strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of <em>Y. lipolytica</em> PO1f cultures by a factor of 30.</div></div><div><h3>Conclusions</h3><div>ISY medium was suitable not only for the cultivation of <em>Y. lipolytica</em> but also universally applicable for <em>Yarrowia</em>-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.</div><div><strong>How to cite:</strong> Birrenbach O, Czermak P. Development of a chemically defined medium for <em>Yarrowia</em> yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 32-37"},"PeriodicalIF":2.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes","authors":"Mohammad J. Alsarraf ,&nbsp;Fuad Ameen ,&nbsp;Abdullah Alfalih ,&nbsp;Zirak Sajjad","doi":"10.1016/j.ejbt.2024.09.001","DOIUrl":"10.1016/j.ejbt.2024.09.001","url":null,"abstract":"<div><h3>Background</h3><div>To address public health challenges, there is increasing interest in bioactive metabolites from unconventional sources for targeted cancer and viral treatments with minimal side effects. Endophytic fungi are promising for these therapies. This study focused on maximizing bioactive agent yields from these fungi using a low-cost medium and optimized fermentation system.</div></div><div><h3>Results</h3><div>Forty-three endophytic fungi isolated from <em>Sinularia polydactyla</em> were cultivated on wheat bran medium and evaluated for bioactive metabolites under solid-state fermentation. Strain FAKSA 10, identified as <em>Arthrinium</em> sp. FAKSA 10, exhibited the highest levels of pharmaceutical metabolites, including L-glutaminase, L-methioninase, L-arginase, L-asparaginase, L-tyrosinase, L-lysine α-oxidase, and ribonuclease, with a 79.12% hepatitis C virus knockdown rate. This strain produced 46 metabolites with anticancer, antioxidant, antiviral, and cytotoxic properties, including major compounds like hexadecanoic acid methyl ester; hexadecanoic acid ethyl ester; 9, 12-octadecadienoic acid (Z, Z), methyl ester; 9-octadecenoic acid (Z)-, methyl ester, and 11, 14-eicosadienoic acid, methyl ester. A cost-effective strategy using 11 agro-industrial residues was applied, followed by optimization of the solid-state fermentation system. This optimization increased enzyme yields and enhanced antiviral and antioxidant activities. The optimal conditions for solid-state fermentation were a 10 d incubation, 1 mm particle size, 60% initial moisture, 28 °C temperature, and 2 × 10⁷ CFU/mL inoculum size.</div></div><div><h3>Conclusions</h3><div>This study exploited <em>Arthrinium</em> sp. FAKSA 10 metabolites as natural pharmaceuticals against cancer and viral diseases, highlighting their significant antioxidant properties. Among various residues, oil cakes emerged as an effective and cost-efficient medium, capable of significantly enhancing the production of valuable bioactive metabolites.</div><div><strong>How to cite:</strong> Alsarraf MJ, Ameen F, Alfalih A, et al. Evaluation of high-value bioproducts production by marine endophytic fungus <em>Arthrinium</em> sp. FAKSA 10 under solid state fermentation using agro-industrial wastes. Electron J Biotechnol 2024;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.09.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 1-17"},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic evaluation of the nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated in the biocompatible hydrogel poly(VCL-co-PEGDA) based on photodynamic effect","authors":"Coralia Cuadrado ,&nbsp;Alex Mantilla ,&nbsp;Luis Hidalgo ,&nbsp;Diego Punina","doi":"10.1016/j.ejbt.2024.08.002","DOIUrl":"10.1016/j.ejbt.2024.08.002","url":null,"abstract":"<div><h3>Background</h3><div>The rise of drug-resistant bacteria, including <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, presents a significant healthcare challenge. This study focuses on the development of two novel nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated within a biocompatible poly(VCL-co-PEGDA) hydrogel. These composites are designed for use in photodynamic therapy and evaluated for their antimicrobial efficacy against resistant pathogens.</div></div><div><h3>Results</h3><div>The synthesized nanocomposites, when irradiated with red light at 630 nm, showed significant antimicrobial activity, resulting in a marked reduction in the viability of <em>S. aureus</em> ATCC 27543, <em>S. aureus</em> ATCC 33591, and <em>E. coli</em> ATCC 971182. Photodynamic studies demonstrated that the IONs-GO-Pc nanocomposite was more efficient in generating singlet oxygen compared to IONs-MWCNTs-Pc, which correlated with its superior antimicrobial performance. Structural and chemical characterization confirmed the successful incorporation of nanomaterials and photosensitizers, enhancing the photodynamic effect.</div></div><div><h3>Conclusions</h3><div>The study demonstrates that both IONs-MWCNTs-Pc and IONs-GO-Pc nanocomposites show promise as alternative treatments for infections caused by antibiotic-resistant bacteria, with the GO-based composite showing higher photodynamic therapy efficacy. These findings suggest that such nanocomposites could play a pivotal role in advancing antimicrobial strategies against resistant pathogens.</div><div><strong>How to cite:</strong> Cuadrado C, Mantilla A, Hidalgo L, et al. Antibiotic evaluation of the nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated in the biocompatible hydrogel poly(VCL-co-PEGDA) based on photodynamic effect. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.08.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 18-31"},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The significance of chemical transfection/transduction enhancers in promoting the viral vectors-assisted gene delivery approaches: A focus on potentials for inherited retinal diseases","authors":"Sajad Najafi ,&nbsp;Azam Rahimpour ,&nbsp;Hamid Ahmadieh ,&nbsp;Maryam Maleki Tehrani ,&nbsp;Mohammad Amin Khalilzad ,&nbsp;Fatemeh Suri ,&nbsp;Javad Ranjbari","doi":"10.1016/j.ejbt.2024.07.005","DOIUrl":"10.1016/j.ejbt.2024.07.005","url":null,"abstract":"<div><div>Viral vectors are among the main approaches currently used in studies for executing gene delivery to target cells. During the past decades of active studies in gene therapy, including viruses, adenoviruses (Ads), lentiviruses (LVs), and adeno-associated viruses (AAVs), have received the most attention among the biological approaches where potentially successful outcomes are recorded for numerous genetic conditions. The success of delivery methods, however, remains unsatisfactory. Using some additives that can improve transgene expression, transfection efficiency, viral particle production, and transduction efficiency is considered as a solution to overcoming the limitations of gene delivery approaches. These additives include caffeine, histone deacetylase (HDAC) inhibitors like sodium butyrate and valproic acid, and polycationic agents like polybrene and protamine sulfate. In this review article, we present an overview of viral vector-mediated retinal gene therapies and the application of some enhancers used to improve the outcomes of gene delivery. Three routes of administrating viral vectors into ocular compartments are employed for the delivery of target genes into impacted cells, and some additives have shown enhanced efficiency of gene delivery in retinal cells. The current study places a special focus on the viral vectors and enhancers used for gene therapies of inherited retinal diseases.</div><div><strong>How to cite:</strong> Najafi S, Rahimpour A, Ahmadieh H, et al. The significance of chemical transfection/transduction enhancers in promoting the viral vectors-assisted gene delivery approaches: A focus on potentials for inherited retinal diseases. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 29-40"},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Lactobacillus plantarum viability using novel chitosan-alginate-pectin microcapsules: Effects on gastrointestinal survival, weight management, and metabolic health","authors":"Mahla Shahriari ,&nbsp;Ashraf Mohabati Mobarez ,&nbsp;Amin Talebi Bazminabadi ,&nbsp;Masoumeh Tavakoli Yaraki","doi":"10.1016/j.ejbt.2024.07.004","DOIUrl":"10.1016/j.ejbt.2024.07.004","url":null,"abstract":"<div><h3>Background</h3><div>Probiotics, like <em>Lactobacillus plantarum</em>, show promise in managing obesity, but delivery challenges hinder their effectiveness. This study explores the effects of NCAP microcapsules with <em>L. plantarum</em> on weight, lipids, liver function, and insulin in rats over eight weeks, enhancing the bacteria’s survivability through microencapsulation.</div></div><div><h3>Results</h3><div>NCAP microcapsules (10–15 μm, &gt;79% survival) effectively protected L. plantarum. In rats, a high-fat diet with NCAP microcapsules (HFDC) significantly reduced body weight (176 g vs 179 g, <em>p</em> &lt; 0.001), low-density lipoprotein (33 mg/dL vs 44 mg/dL, <em>p</em> &lt; 0.001), and improved liver markers (aspartate transaminase 56 U/L vs 69 U/L, <em>p</em> = 0.008; alanine transaminase 36 U/L vs 38 U/L, <em>p</em> &lt; 0.001). <em>L. plantarum</em> counts were notably higher in HFDC (870,963,590 CFU/g vs 14,454 CFU/g, <em>p</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>NCAP microcapsules enhance <em>L. plantarum</em> survivability and improve weight, lipids, liver function, and insulin. This study addresses the need for effective probiotic delivery systems, offering insights into metabolic health promotion.</div><div><strong>How to cite:</strong> Shahriari M, Mohabati Mobarez A, Talebi Bazminabadi A, et al. Enhancing <em>Lactobacillus plantarum</em> viability using novel chitosan-alginate-pectin microcapsules: Effects on gastrointestinal survival, weight management, and metabolic health. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 20-28"},"PeriodicalIF":2.3,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetylcholinesterase inhibition exerted by the extract of Daldinia eschscholtzii, a marine fungus associated with the coral Siderastrea siderea: GC-MS analysis and molecular docking of identified compounds","authors":"Karla G. Olivo-Flores ,&nbsp;Alan Couttolenc ,&nbsp;Jorge Suárez-Medellín ,&nbsp;Ángel Trigos ,&nbsp;César Espinoza","doi":"10.1016/j.ejbt.2024.08.001","DOIUrl":"10.1016/j.ejbt.2024.08.001","url":null,"abstract":"<div><h3>Background</h3><div>Alzheimer’s disease is a neurodegenerative disease that has no cure. The drugs used to treat Alzheimer’s disease are based on the inhibition of the enzyme acetylcholinesterase (AChE). In this sense, marine fungal metabolites represent an alternative source for the discovery of drugs based on AChE inhibition (IAChE). The present research carried out a bioprospecting study of marine fungi with acetylcholinesterase inhibition potential.</div></div><div><h3>Results</h3><div>A total of 17 marine fungi were isolated from three stony corals from the Veracruz Reef System. The fungal genera identified were <em>Geotrichum</em>, <em>Curvularia</em>, <em>Penicillium</em>, <em>Aspergillus,</em> and <em>Daldinia</em>. The IAChE was evaluated from the broth and biomass extracts of each fungal strain. As a result, <em>Daldinia eschscholtzii</em> was one of the three fungi with the greatest IAChE effect (32.7%). Subsequently, a bio-directed chromatographic purification of the methanolic extract of the biomass of <em>D. eschscholtzii</em> was carried out. The FRL8.1 fraction was the most active with an IAChE of 41.0%, a value close to Galantamine positive control (44.0%). Furthermore, compound analysis was carried out by GC-MS. Finally, it was determined that the metabolites responsible for the inhibitory effect are probably 4,7-dihydroxycoumarin and 5-nitro-2-naphthalenamine, which was demonstrated by their interactions with the AChE enzyme receptor through molecular docking studies.</div></div><div><h3>Conclusions</h3><div>Coral-associated marine fungi produce secondary metabolites that inhibit acetylcholinesterase. This is the first report of <em>D. eschscholtzii</em> isolated from the stony coral <em>Siderastrea siderea</em>. Finally, we consider that more studies are needed to demonstrate the IAChE potential of marine fungi associated with corals or other marine organisms.</div><div><strong>How to cite:</strong> Olivo-Flores KG, Couttolenc A, Suárez-Medellín J, et al. Acetylcholinesterase inhibition exerted by the extract of <em>Daldinia eschscholtzii</em>, a marine fungus associated with the coral <em>Siderastrea siderea</em>: GC–MS analysis and molecular docking of identified compounds. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.08.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 12-19"},"PeriodicalIF":2.3,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000241/pdfft?md5=a2eb14a6458060bbb9dcd2b5e6b04b8c&pid=1-s2.0-S0717345824000241-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial characteristics of endophytic Aspergillus terreus and acute oral toxicity analysis","authors":"Amr M. Shehabeldine ,&nbsp;Amer M. Abdelaziz ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Bushra H. Kiani ,&nbsp;Ahmed S. Hussein","doi":"10.1016/j.ejbt.2024.07.003","DOIUrl":"10.1016/j.ejbt.2024.07.003","url":null,"abstract":"<div><h3>Background</h3><p>Endophytic fungi produce biologically robust metabolites suitable for diverse applications, which support the increasing exploration of these fungi. The study aimed to investigate the <em>in vitro</em> antimicrobial properties of the metabolites of endophytic fungus <em>Aspergillus terreus</em> which isolated from the leaves of <em>Psidium guajava</em> plants while the acute oral toxicity was assessed <em>in vivo.</em></p></div><div><h3>Results</h3><p><em>A. terreus</em> (OR125572) was successfully isolated for the first time from <em>P. guajava</em>. The ethyl acetate extract of <em>A. terreus</em> exhibited antibactericidal effects against <em>Enterobacter aerogenes</em> with a minimum inhibitory concentration of 18.75 mg/ml in contrast to 75.00 ± 0.0, 37.50, and 37.50 mg/ml, respectively against <em>Staphylococcus aureus, Escherichia coli, and Bacillus sphaericus</em> were 75.00 ± 0.0, 37.50, and 37.50 mg/ml, respectively. Analysis of the crude extract obtained from <em>A. terreus</em> by GC-MS revealed a total of 32 distinct compounds. The major components included 1,2-benzenedicarboxylic acid, di-iso-octyl ester; hexyl oxecan-2-one and phenol. The acute oral toxicity study found no symptoms of toxicity and no mortality until the 14th d, suggesting that the LD₅₀ value of <em>A. terreus</em> extract might exceed 1 ml/kg. The group receiving 0.5 ml/kg of <em>A. terreus</em> extract experienced a 42.9% increase in body weight, while administration of varying dosages resulted in a significant reduction in MCV levels. There was also a significant increase in the proportion of monocytes across all treated groups.</p></div><div><h3>Conclusions</h3><p>The study demonstrated the potential of <em>A. terreus</em> as a source of antimicrobial and other bioactive compounds, with relatively low acute toxicity.</p><p><strong>How to cite:</strong> Shehabeldine AM, Abdelaziz AM, Abdel-Maksoud MA, et al. Antimicrobial characteristics of endophytic <em>Aspergillus terreus</em> and acute oral toxicity analysis. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.003</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 1-11"},"PeriodicalIF":2.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S071734582400023X/pdfft?md5=028fec87a343f02b51c4d37f03724724&pid=1-s2.0-S071734582400023X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142130032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}