Electronic Journal of Biotechnology最新文献

{"title":"Metabolites of native actinomycetes from Sof Umer cave reveal potent antimicrobial activity against selected pathogens in mice infection models","authors":"Ebisa Chaluma Abdeta ,&nbsp;Abu Feyisa Meka ,&nbsp;Daniel Girma Hordofa ,&nbsp;Belete Ketema Sime ,&nbsp;Gadisa Abdisa Akkewak ,&nbsp;Jemal Ali Mahdi ,&nbsp;Musin Kelel Abas ,&nbsp;Mesfin Tafesse Gemeda","doi":"10.1016/j.ejbt.2025.100703","DOIUrl":"10.1016/j.ejbt.2025.100703","url":null,"abstract":"<div><h3>Background</h3><div>Actinomycetes are gram-positive bacteria that belong to the actinobacterial species. They are a prolific source of secondary metabolites with various biological applications. Thus, this study aimed to culture-based isolation of potent Actinomycete species from Sof-Umer Cave and <em>in vitro</em> and <em>in vivo</em> evaluation of their potential metabolites against selected test organisms.</div></div><div><h3>Result</h3><div>Among the total isolates, ten isolates were selected based on their antimicrobial activities. Among them, the ethyl acetate crude extract of three isolates (RO13, SD2, R011) showed potential antagonistic activity, ranging from 17 ± 0.78 to 23 ± 0.56 mm of zone of inhibition against <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Staphylococcus aureus.</em> Additionally, two isolates’ (SD2, R011) crude extract exhibited significant inhibition of test organisms in wound and oral infection of the mice models. This was confirmed by wound contraction and progress improvement of the clinical sign observed before treatment. Characterization of their crude extract by FTIR and GC–MS revealed the presence of various functional groups and compounds. Specifically, potent antimicrobial and antioxidant bioactive compounds, such as pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-2-piperidine, phenol, 2-methoxy-4-(1-propenyl)-, and indolizine, were identified via GC–MS analysis. Three of the ten potent isolates (R013, R011, and SD2) were identified based on the 16S rRNA gene sequence, and the R013 isolate belongs to <em>Streptomycetes flavoviridis</em>, whereas SD2 and R011 were identified as <em>Arthrobacter</em> sp. and <em>Actinobacterium kmd_152</em>, respectively.</div></div><div><h3>Conclusions</h3><div>Sof-Umer cave-dwelling actinomycetes possess potent metabolites against test organisms that can be a base for future potent drug development against microbial infections.</div><div><strong>How to cite:</strong> Abdeta EC, Meka AF, Hordofa DG, et al. Metabolites of native actinomycetes from Sof Umer cave reveal potent antimicrobial activity against selected pathogens in mice infection models. Electron J Biotechnol 2026;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100703</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100703"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HRMS-based profiling of metabolites, metal ions content and in-vitro cholinesterase inhibitory activities of Sonchus wightianus DC plant parts","authors":"Abhimat Subedi ,&nbsp;Bishnu Prasad Pandey ,&nbsp;Suman Prakash Pradhan ,&nbsp;G.C. Ashok ,&nbsp;Sumit Bhattarai ,&nbsp;Ankita Dahal ,&nbsp;Era Tuladhar ,&nbsp;Anupama Chapagain ,&nbsp;Mukti Ram Aryal ,&nbsp;Gopal Prasad Ghimire","doi":"10.1016/j.ejbt.2025.100702","DOIUrl":"10.1016/j.ejbt.2025.100702","url":null,"abstract":"<div><h3>Background</h3><div><em>Sonchus wightianus</em> DC is native to South Asia and has traditionally been known for its wide range of applications for the treatment of several human ailments. However, its application for the treatment of neurodegenerative diseases like Alzheimer’s disease (AD) has not been studied yet. In this present study, comprehensive metabolite profiling of plant parts and <em>in-vitro</em> cholinesterase inhibitory potential was examined to see the efficacy of plant extract against AD.</div></div><div><h3>Results</h3><div>The potent antioxidant activity was demonstrated by the flower extract in both DPPH and ABTS assays, with IC₅₀ values of 104.06 ± 2.05 µg/mL and 67.69 ± 1.58 µg/mL, respectively. The crude methanol extract of the leaf displayed the highest butyrylcholinesterase (BChE) inhibition potential with IC₅₀ values of 281.09 ± 14.64 µg/mL. In contrast, the flower extract exhibited the strongest acetylcholinesterase (AChE) inhibition with IC₅₀ values of 247.51 ± 11.15 µg/mL. Furthermore, the evaluated plant parts were a rich source of essential macro and micronutrients. Principal component analysis revealed the major contribution of total phenolic content (TPC), and total flavonoid content (TFC) in the plant extracts, which might be the prime reason for strong antioxidant and cholinesterase inhibition. Further, the HRMS profiling analysis revealed the presence of Linoleic acid, gingerol, kaempferol, genistein, daidzein, chlorogenic acid, fisetin and 12-oxo-phytodienoic acid.</div></div><div><h3>Conclusions</h3><div>The findings of this study suggest that <em>Sonchus wightianus</em> DC is a promising source of bioactive compounds and essential micronutrients, with notable potential as an anticholinesterase agent.</div><div><strong>How to cite:</strong> Subedi A, Pandey BP, Pradhan SP, et al. HRMS-based profiling of metabolites, metal ions content and <em>in-vitro</em> cholinesterase inhibitory activities of <em>Sonchus wightianus</em> DC plant parts. Electron J Biotechnol 2026;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100702</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100702"},"PeriodicalIF":2.5,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anoikis- and m6A-related lncRNA analysis to identify prognostic indicators in liver hepatocellular carcinoma","authors":"Pan Yu ,&nbsp;Shuaiyang Jing ,&nbsp;Sarinder Kaur Dhillon","doi":"10.1016/j.ejbt.2025.100701","DOIUrl":"10.1016/j.ejbt.2025.100701","url":null,"abstract":"<div><h3>Background</h3><div>In cancer, the process of anoikis is intimately associated with the emergence and progression. N6-methyladenosine modification and m6A modification play an important role in regulating long non-coding RNAs. The liver hepatocellular carcinoma patients’ data, including clinical and prognostic data, were obtained via The Cancer Genome Atlas database. The univariate, multivariate Cox and Least Absolute Selection Operator (LASSO) regression were performed to gain anoikis- and m6A-related lncRNAs. The Kaplan-Meier method was employed to assess the overall survival rate for groups of high- and low risks.</div></div><div><h3>Results</h3><div>A signature comprising six anoikis- and m6A-related lncRNAs was constructed: AL117336.3, LINC01138, Z83851.1, NRAV, CASC19 and AC009283.1. The clinicopathological variables, the anoikis- and m6A-related lncRNA signature demonstrated superior diagnostic efficacy, with an area under the receiver operating characteristic curve of 0.810. In the high-risk group, the overall survival was shown to be inferior to that of in group of low risk, while patients were classified by distinct clinicopathological variables. The ssGSEA and CIBERSORT immune analysis demonstrated that the predictive signature was significantly associated with liver cancer patients’ immune status. The chemotherapy drugs ATRA, AUY922, bexarotene, gemcitabine, mitomycin-C, and PHA have been found to have greater sensitivity in treating high-risk patients. qRT-PCR showed that Z83851.1, NRAV and CASC19 lncRNAs were associated with poor prognosis and were high-risk factors. AC009283.1 lncRNA may have anti-cancer properties.</div></div><div><h3>Conclusions</h3><div>The predictive signature is capable of independently predicting the prognosis of liver cancer patients for understanding the mechanisms of anoikis- and m6A-related lncRNAs in liver hepatocellular carcinoma and offering clinical guidance to patients with liver cancer.</div><div><strong>How to cite:</strong> Yu P, Jing S, Dhillon SK. Anoikis and m6A related lncRNAs analysis to identify prognostic indicators in liver hepatocellular carcinoma. Electron J Biotechnol 2026;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100701</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100701"},"PeriodicalIF":2.5,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost hybrid copper–carbon nanotube coating with antimicrobial properties in ambient conditions","authors":"Christian Pablo Romero ,&nbsp;Claudio Ramirez-Mora ,&nbsp;Rodolfo Salazar ,&nbsp;Cristobal Fernandez ,&nbsp;Cristian Acevedo ,&nbsp;Christian Orellana ,&nbsp;Maria Abellan ,&nbsp;David Aliaga","doi":"10.1016/j.ejbt.2025.100700","DOIUrl":"10.1016/j.ejbt.2025.100700","url":null,"abstract":"<div><h3>Background</h3><div>The development of bactericidal surfaces using nanotechnology has gained traction in high-tech sectors due to their effectiveness against pathogens. However, widespread adoption in low-income regions remains limited by the high cost of materials such as copper nanoparticles and the need for specialized application personnel. This study aims to develop a cost-effective bactericidal coating that minimizes nano-copper usage while maintaining strong antimicrobial performance and practical applicability in resource-limited environments.</div></div><div><h3>Results</h3><div>A polymer-based coating incorporating ≤3 wt% nano-copper and carbon nanotubes was formulated to enhance conductivity and mechanical stability. The fabrication process was optimized for on-site application under ambient conditions. Scanning Electron Microscopy (SEM) revealed a uniform surface distribution of nano-copper particles. Bactericidal activity tests confirmed efficacy against <em>Escherichia coli</em>, <em>Listeria monocytogenes</em>, and <em>Salmonella</em> spp. Techno-economic analysis indicated that the coating could be integrated into existing surface finishing systems at an incremental cost of 2.6–3.5 USD per gallon.</div></div><div><h3>Conclusions</h3><div>This work demonstrates the feasibility of producing and applying affordable nano-based bactericidal coatings under real-world conditions. The approach provides a practical pathway for implementing antimicrobial surface technologies in low-resource settings. Although the present study focused on wood substrates, future research should assess performance on diverse materials to broaden applicability. The combination of cost-effectiveness, efficacy, and scalability underscores the potential for both commercial adoption and significant public health benefits.</div><div><strong>How to cite:</strong> Romero CP, Ramirez-Mora C, Salazar R, et al. Low-cost hybrid copper–carbon nanotube coating with antimicrobial properties in ambient conditions. Electron J Biotechnol 2026;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100700</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100700"},"PeriodicalIF":2.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can broad bean (Vicia faba) and white lupin (Lupinus albus) flours serve as carbon sources to support probiotic growth?","authors":"Evla Vieira,&nbsp;Marta W. Vasconcelos,&nbsp;Ana Maria Gomes","doi":"10.1016/j.ejbt.2025.100697","DOIUrl":"10.1016/j.ejbt.2025.100697","url":null,"abstract":"<div><h3>Background</h3><div>There is growing interest in identifying substrates that support the growth of probiotics in foods. Pulses are an excellent source of nutrients and bioactive compounds, including non-digestible oligosaccharides from the α-galactoside group, which are probiotic growth factors. This study aimed to evaluate the potential of white lupin and broad bean flours to support the growth of seven probiotic strains of <em>Lactobacilli</em> and <em>Bifidobacterium</em>.</div></div><div><h3>Results</h3><div>Different Man-Rogosa-Sharpe broth media were prepared using whole or dehulled flour as carbon sources at different concentrations (20, 30, 40, and 60 g/L) and inoculated with 2% (w/v) of each probiotic strain. Viable cell numbers and medium acidification were monitored throughout fermentation and compared to negative (MRS without a carbon source) and positive (MRS with 20 g/L glucose) controls. White lupin at 60 g/L concentration proved to be a suitable carbon source for both <em>Lactobacillus acidophilus</em> Ki and <em>Lactobacillus casei</em> ssp. <em>paraca</em>sei L26, while concentrations of 40 g/L and 60 g/L supported <em>Bifidobacterium animalis</em> Bb12 growth.</div></div><div><h3>Conclusions</h3><div>Flour concentration had a greater impact on probiotic growth than composition (hull vs. dehulled). These results suggested that white lupin is a promising ingredient for the development of functional foods.</div><div><strong>How to cite:</strong> Vieira E, Vasconcelos MW, Gomes AM. Can broad bean (<em>Vicia faba</em>) and white lupin (<em>Lupinus albus</em>) flours serve as carbon sources to support probiotic growth? Electron J Biotechnol 2026;5;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100697</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100697"},"PeriodicalIF":2.5,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obituary for James D. Watson (1928–2025)","authors":"Graciela Muñoz-Riveros","doi":"10.1016/j.ejbt.2025.100699","DOIUrl":"10.1016/j.ejbt.2025.100699","url":null,"abstract":"","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100699"},"PeriodicalIF":2.5,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis of silver nanoparticles using cell-free extracts of thermotolerant bacteria: Antioxidant and antibacterial properties","authors":"Aparna Banerjee ,&nbsp;Ismael Herrera-Vargas ,&nbsp;Mario E. Flores ,&nbsp;Francisca Valenzuela ,&nbsp;Srijan Banerjee","doi":"10.1016/j.ejbt.2025.100698","DOIUrl":"10.1016/j.ejbt.2025.100698","url":null,"abstract":"<div><h3>Background</h3><div>Eco-friendly synthesis of silver nanoparticles (AgNPs) using biological systems offers a sustainable alternative to conventional physicochemical methods. In this study, we employed cell-free extracts from three thermotolerant bacterial strains, <em>Bacillus haynesii</em> CamB6, <em>Pseudomonas alcaligenes</em> Med1, and <em>Staphylococcus</em> sp. BSP3 for the biosynthesis of AgNPs, aiming to explore their antioxidant and antibacterial properties.</div></div><div><h3>Results</h3><div>The biosynthesized AgNPs were characterized through UV–Vis spectroscopy, FTIR, TEM, and DLS analyses, which revealed distinct physicochemical profiles among the nanoparticles. Notably, AgNP2 and AgNP3 exhibited smaller particle sizes, enhanced colloidal stability, and superior biological activities compared to AgNP1. Antioxidant evaluation demonstrated significant free radical scavenging potential, with AgNP2 showing the highest DPPH activity (65.18% at 5 mg mL⁻¹). Antibacterial activity, assessed via agar well diffusion and cell viability assays against <em>Bacillus cereus</em> and <em>Pseudomonas putida</em> revealed that AgNP2 achieved the lowest bacterial viability (0.74%) for <em>P. putida</em> at 1 mg mL⁻¹ concentration.</div></div><div><h3>Conclusions</h3><div>The study highlights the potential of biosynthesized AgNPs, particularly AgNP2, as sustainable for biomedical applications. Their antioxidant and antibacterial activities suggest valuable applications in managing oxidative stress and combating antimicrobial resistance.</div><div><strong>How to cite:</strong> Banerjee A, Herrera-Vargas I, Flores ME, et al. Biogenic synthesis of silver nanoparticles using cell-free extracts of thermotolerant bacteria: Antioxidant and antibacterial properties. Electron J Biotechnol 2026:79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100698</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100698"},"PeriodicalIF":2.5,"publicationDate":"2025-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANKRD1 knockdown attenuates doxorubicin-induced dilated cardiomyopathy by regulating mitochondrial dysfunction and oxidative stress through activation of the AMPK/AKT/mTOR pathway","authors":"Jia Yuan ,&nbsp;Yu Zhou ,&nbsp;GuoHua Wei ,&nbsp;Tao Qi ,&nbsp;HaoLiang Sun ,&nbsp;Jian Shen","doi":"10.1016/j.ejbt.2025.08.002","DOIUrl":"10.1016/j.ejbt.2025.08.002","url":null,"abstract":"<div><h3>Background</h3><div>Doxorubicin (DOX), a widely used chemotherapeutic agent, causes severe cardiotoxicity that frequently progresses to dilated cardiomyopathy (DCM). While ankyrin repeat domain 1 protein (ANKRD1) plays critical roles in cardiovascular pathophysiology, its specific involvement in doxorubicin-induced DCM remains unknown. This study investigates the functional significance of ANKRD1 in DOX-induced DCM pathogenesis.</div></div><div><h3>Results</h3><div>DOX treatment significantly upregulated ANKRD1 expression in both rat models and H9c2 rat cardiomyocytes. <em>In vivo</em>, ANKRD1 knockdown ameliorated DOX-induced cardiac dysfunction, as demonstrated by improved left ventricular ejection fraction and fractional shortening, along with reduced serum levels of lactate dehydrogenase and creatine kinase-myocardial band. Conversely, ANKRD1 overexpression exacerbated cardiac impairment. Pathological examination revealed that ANKRD1 knockdown attenuated DOX-induced myocardial tissue damage and collagen deposition, while ANKRD1 overexpression intensified these pathological changes. Furthermore, ANKRD1 knockdown mitigated mitochondrial dysfunction and oxidative stress in DCM models both <em>in vivo</em> and <em>in vitro</em>. Mechanistically, ANKRD1 knockdown activated the AMP-activated protein kinase (AMPK)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling cascade, thereby attenuating DOX-induced cardiomyocyte toxicity, mitochondrial dysfunction, and oxidative stress. Rescue experiments using the AMPK inhibitor dorsomorphin confirmed this pathway’s involvement, as dorsomorphin treatment abolished the protective effects of ANKRD1 knockdown against DOX-induced cardiomyocyte damage.</div></div><div><h3>Conclusions</h3><div>ANKRD1 knockdown prevents DOX-induced DCM by ameliorating mitochondrial dysfunction and oxidative stress through activation of the AMPK/AKT/mTOR pathway. These findings establish ANKRD1 as a promising therapeutic target for preventing DOX-induced cardiotoxicity and DCM.</div><div><strong>How to cite:</strong> Yuan J, Zhou Y, Wei G, et al. ANKRD1 knockdown attenuates doxorubicin-induced dilated cardiomyopathy by regulating mitochondrial dysfunction and oxidative stress through activation of the AMPK/AKT/mTOR pathway. Electron J Biotechnol 2026, 79. <span><span>https://doi.org/10.1016/j.ejbt.2025.08.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100696"},"PeriodicalIF":2.5,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis","authors":"Meng-qi Li ,&nbsp;Yu-cheng Lu ,&nbsp;Yan-chun Li ,&nbsp;Yu-fu Zeng ,&nbsp;Ying-ying Cao ,&nbsp;Ling Zhang ,&nbsp;Ben Chen ,&nbsp;Ling Chen ,&nbsp;Wei-an Qiu ,&nbsp;Zi-heng Huang","doi":"10.1016/j.ejbt.2025.08.001","DOIUrl":"10.1016/j.ejbt.2025.08.001","url":null,"abstract":"<div><h3>Background</h3><div>Kaixin San (KXS), a traditional Chinese herbal formula, is used to treat vascular dementia (VaD), but its active ingredients and mechanisms remain unclear. This study combined animal experiments with network pharmacology to explore how KXS modulates the Bcl-2/Beclin-1/LC3A/B pathway in VaD treatment.</div></div><div><h3>Results</h3><div>LC‒MS/MS identified 164 active ingredients in the KXS ethanol extract. In 2-vessel occlusion model rats, KXS significantly improved learning and memory (<em>p</em> &lt; 0.05 or <em>p</em> &lt; 0.01) and reduced hippocampal CA1 neuronal damage. Western blotting showed KXS upregulated Bcl-2/Bcl-XL and downregulated Beclin-1, LC3A/B, and Bax (<em>p</em> &lt; 0.05). Immunofluorescence confirmed increased Bcl-2 and decreased Beclin-1 expression. Network pharmacology predicted 73 targets and 174 pathways, with TNF, AKT1, IL-1β, PTGS2, and ESR1 as key targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis linked these targets to cancer, atherosclerosis, and AGE-RAGE signaling pathways.</div></div><div><h3>Conclusions</h3><div>KXS alleviates VaD by modulating autophagy and apoptosis via Bcl-2/Beclin-1/LC3A/B, with multi-target, multi-pathway effects. These findings support further investigation of KXS as a potential therapy for VaD.</div><div><strong>How to cite:</strong> Li M, Lu Y, Li Y, et al. Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.08.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 96-109"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145413009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway","authors":"Yanlin Tan ,&nbsp;Jun Gao","doi":"10.1016/j.ejbt.2025.07.006","DOIUrl":"10.1016/j.ejbt.2025.07.006","url":null,"abstract":"<div><h3>Background</h3><div>N6-methyladenosine (m⁶A) methylation plays a key role in osteosarcoma (OS) progression. This study aimed to elucidate the function and mechanism of methyltransferase 16 (METTL16), an m⁶A methyltransferase, in OS progression.</div></div><div><h3>Results</h3><div>Bioinformatics analysis with quantitative reverse-transcription polymerase chain reaction (qRT-PCR) revealed high METTL16 expression in OS. After performing cell functional experiments, METTL16 silencing was shown to decrease the proliferation, migration, and invasion of OS cells. Using qRT-PCR, methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR), Western blotting, luciferase, RNA-binding protein immunoprecipitation (RIP), and RNA stability assays, METTL16 induced the m⁶A methylation of ubiquitin protein ligase E3A (UBE3A) to promote UBE3A expression and mRNA stability in OS cells in a fragile X messenger ribonucleoprotein 1 (FMR1)-dependent manner. Moreover, <em>in vitro</em> and <em>in vivo</em> results showed that UBE3A activated the Notch signaling pathway, thereby promoting OS cell malignancy. METTL16 knockdown partly reversed the oncogenic role of UBE3A in OS cells.</div></div><div><h3>Conclusions</h3><div>METTL16 acts as a tumor promotor in OS progression by modulating UBE3A expression via m⁶A methylation to activate the Notch signaling pathway. The findings highlight the therapeutic potential of disrupting the METTL16–UBE3A–Notch pathway axis in OS.</div><div><strong>How to cite:</strong> Tan Y, Gao J. METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 86-95"},"PeriodicalIF":2.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}