Precisely mimicking lipoxidase with histidine coordinated Fe-MOF for temperature-adaptive antiviral protection

Abstract

Highly pathogenic viruses are a global challenge for human health, as they are easy to spread, prone to mutation and thus difficult to prevent. Novel strategy that can effectively disrupt viruses is urgently needed. Nanozymes, especially with oxidase-like activity, have shown great potential in inactivating viruses with lipid envelope. However, the design of oxidase-like nanozymes lacks rational strategy and thus their activity is nonspecific. Inspired by natural lipoxidase or lipoxygenase (LOX) enzyme, we design an ultrasonic method to synthesize a novel histidine-decorated metal-organic framework nanozyme based on nonheme iron (Fe MOF@His) in this work. Fe MOF@His with intrinsic lipoxidase-like activity not only exhibits high catalytic activity towards two kinds of substrates containing polyunsaturated fatty acids (PUFA) within a broad range of temperature and pH value, but also presents excellent catalytic specificity to the linoleic acid instead of traditional peroxidase and oxidase substrate such as TMB (3,3′,5,5′-Tetramethylbenzidine). Based on its high lipoxidase-like activity, we find Fe MOF@His has excellent antiviral ability against influenza viruses and could maintain its lethality at 4°C and −20°C, which can provide effective antiviral protection for solid and fabric surfaces. Collectively, Fe MOF@His has good specificity, high catalytic activity and stability over time as a novel lipoxidase-like nanozyme, which provides a new antiviral agent adapted to different environments.