Electrospinning of Self-Assembling Oligopeptides into Nanofiber Mats: The Impact of Peptide Composition and End Groups

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-05 DOI:10.1021/acs.biomac.4c01401

Prerana Rathore , Brian Montz , Shao-Hsiang Hung , Pankaj Kumar Pandey , Sarah L. Perry , Todd Emrick , Jessica D. Schiffman

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Abstract

Low-molecular-weight oligopeptides can be electrospun into nanofiber mats. However, the mechanism underlying their electrospinnability is not well-understood. In this study, we used solid-phase peptide synthesis to produce the oligopeptide FFKK, to which the aromatic end-capping groups naphthalene, pyrene, and tetraphenylporphyrin were attached. Nuclear magnetic resonance, circular dichroism, and electrospray ionization mass spectrometry were used to characterize the oligopeptide structures. We investigated the effect of end-caps and oligopeptide concentration on their self-assembly as well as on their electrospinnability in fluorinated solvents. All oligopeptides with aromatic end-caps were amenable to electrospinning. Attenuated total reflectance Fourier transform infrared spectroscopy and microrheology results support the hypothesis that at sufficiently high concentrations, the self-assembled structures interact strongly, which facilitates electrospinning. Moreover, the results from this fundamental study can be extended to nonpeptidic small molecules possessing strong intermolecular interactions.

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将自组装的寡肽电纺成纳米纤维垫：肽的组成和末端基团的影响。

低分子量的寡肽可以被电纺成纳米纤维垫。然而，其电可纺性的机制尚不清楚。在本研究中，我们采用固相多肽合成的方法合成了寡肽FFKK，其芳香端盖基萘、芘和四苯基卟啉分别连接在FFKK上。利用核磁共振、圆二色性和电喷雾电离质谱对其结构进行了表征。我们研究了端帽和寡肽浓度对其自组装以及在氟化溶剂中电可纺性的影响。所有具有芳香端帽的寡肽都适合静电纺丝。衰减全反射傅立叶变换红外光谱和微流变学结果支持在足够高的浓度下，自组装结构相互作用强烈，有利于静电纺丝的假设。此外，这项基础研究的结果可以扩展到具有强分子间相互作用的非肽小分子。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.