Experimental Study of the Biological Outcome After Free Transplantation of Autologous Auricular Cartilage.

IF 2 3区医学 Q2 SURGERY Aesthetic Plastic Surgery Pub Date : 2025-02-04 DOI:10.1007/s00266-025-04684-4

You Lin, LiYuan Zhang, Zaihong Chen, Zhiyuan Jiang, Wei Cui, Yang Sheng, Zhen Cai

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Abstract

Background: Auricular cartilage is widely used in rhinoplasty, but there are few studies on its biological changes after transplantation.

Objectives: This study investigated the biological transition of cartilage free grafts with and without perichondrium, aiming to provide some theoretical basis for the selection of nasal cartilage stent.

Methods: Spare auricular cartilage from 14 patients undergoing postoperative auricular cartilage augmentation was prepared into two with-/without-perichondrium cartilage pieces of approximately 5*5 mm, which were implanted into the loose connective tissue layer of the left and right sides of the back of the same nude mouse. The auricular cartilage tissues were harvested 2 months later.

Results: Two months after transplantation, there was no obvious resorption in the with-perichondrium group and a small part of the edge of the without-perichondrium group became dull. The number of chondrocytes before and after transplantation was statistically significant (F = 6.88, P < 0.05). After transplantation, the extracellular matrix of chondrocytes was strongly secreted, and the content of collagen fibers was not significantly changed, but the arrangement showed a tendency of disarrangement and rearrangement, which seems more obvious in the without-perichondrium group. There were significant changes in the expression of collagen II protein in the auricular cartilage matrix before and after transplantation (F = 3.41, P < 0.05).

Conclusion: Autologous auricular cartilage survived well for 2 months after free transplantation, but the internal microstructure changed. The quality of auricular cartilages with perichondrium after transplantation is better than without. The perichondrium can support and protect the transplanted cartilage, so it is advisable to retain perichondrium in clinical rhinoplasty.

Level of evidence iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

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背景：耳廓软骨被广泛应用于鼻整形术中，但有关其移植后生物学变化的研究很少：本研究探讨了有软骨周膜和无软骨周膜的软骨游离移植的生物学转变，旨在为鼻软骨支架的选择提供一些理论依据：方法：将14例耳廓软骨隆鼻术后患者的备用耳廓软骨制备成两块约5*5毫米的带/不带软骨周围的软骨片，分别植入同一裸鼠背部左右两侧的疏松结缔组织层。两个月后收获耳廓软骨组织：结果：移植两个月后，有软骨膜组的耳廓软骨无明显吸收，无软骨膜组的耳廓软骨边缘有一小部分变得暗淡无光。移植前后的软骨细胞数量差异有统计学意义（F = 6.88，P 结论：移植前后的软骨细胞数量差异有统计学意义（F = 6.88，P 结论：移植前后的软骨细胞数量差异有统计学意义（F = 6.88，P 结论）：自体耳廓软骨游离移植后两个月存活良好，但内部微观结构发生了变化。移植后有软骨周围的耳廓软骨质量优于无软骨周围的耳廓软骨。软骨周层可以支撑和保护移植的软骨，因此临床鼻整形术中最好保留软骨周层。证据级别iv：本期刊要求作者为每篇文章指定证据级别。有关这些循证医学等级的完整描述，请参阅目录或在线作者须知 www.springer.com/00266 。

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来源期刊

Aesthetic Plastic Surgery 医学-外科

CiteScore

4.40

自引率

25.00%

发文量

479

审稿时长

3 months

期刊介绍： Aesthetic Plastic Surgery is a publication of the International Society of Aesthetic Plastic Surgery and the official journal of the European Association of Societies of Aesthetic Plastic Surgery (EASAPS), Società Italiana di Chirurgia Plastica Ricostruttiva ed Estetica (SICPRE), Vereinigung der Deutschen Aesthetisch Plastischen Chirurgen (VDAPC), the Romanian Aesthetic Surgery Society (RASS), Asociación Española de Cirugía Estética Plástica (AECEP), La Sociedad Argentina de Cirugía Plástica, Estética y Reparadora (SACPER), the Rhinoplasty Society of Europe (RSE), the Iranian Society of Plastic and Aesthetic Surgeons (ISPAS), the Singapore Association of Plastic Surgeons (SAPS), the Australasian Society of Aesthetic Plastic Surgeons (ASAPS), the Egyptian Society of Plastic and Reconstructive Surgeons (ESPRS), and the Sociedad Chilena de Cirugía Plástica, Reconstructiva y Estética (SCCP). Aesthetic Plastic Surgery provides a forum for original articles advancing the art of aesthetic plastic surgery. Many describe surgical craftsmanship; others deal with complications in surgical procedures and methods by which to treat or avoid them. Coverage includes "second thoughts" on established techniques, which might be abandoned, modified, or improved. Also included are case histories; improvements in surgical instruments, pharmaceuticals, and operating room equipment; and discussions of problems such as the role of psychosocial factors in the doctor-patient and the patient-public interrelationships. Aesthetic Plastic Surgery is covered in Current Contents/Clinical Medicine, SciSearch, Research Alert, Index Medicus-Medline, and Excerpta Medica/Embase.