{"title":"Different performance of tea plants to shade based on key metabolites and transcriptome profiles: case study of cultivars Longjing 43 and Yabukita.","authors":"Wen-Xuan Li, Qi-Ting Fang, Qian-Ou Han, Hui-Hui Huang, Xin-Qiang Zheng, Jian-Liang Lu, Yue-Rong Liang, Jian-Hui Ye","doi":"10.1111/ppl.70103","DOIUrl":null,"url":null,"abstract":"<p><p>Shading is widely used in tea cultivation to improve quality by modulating various metabolic pathways in tea plants. However, the differential sensitivity of specific metabolites and the cultivar-dependent responses to shading are not yet fully understood. This study examined the impact of shading on the chemical composition and transcriptional profiles of cv. Longjing 43 and cv. Yabukita. Among the main quality-related compounds, flavonol glycosides were highly responsive to shading, while catechins displayed distinct cultivar-specific responses. KEGG enrichment analysis revealed that flavonoid biosynthesis was the key secondary metabolic difference between cv. Longjing and cv. Yabukita plants under the sunlight, and shading regulated flavonoid biosynthetic pathways in both cultivars. The genes such as ANTHOCYANIDIN REDUCTASE (CsANR) and ANTHOCYANIDIN SYNTHASE (CsANS) were less sensitive to shade in cv. Longjing 43 than cv. Yabukita, leading to relatively higher levels of epi-type catechins in the shade-treated cv. Longjing 43 sample. Additionally, the UVR8-mediated light signaling pathway demonstrated cultivar-specific expression patterns, although the functional roles of key signaling proteins were conserved across both cultivars. The insights into the chemical and molecular responses of tea plants to shading deepen our understanding of the mechanisms driving the cultivar-dependent behaviors of flavonoids, which offers valuable applications for maintaining consistent matcha quality and informing breeding programs of matcha.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70103"},"PeriodicalIF":5.4000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70103","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Shading is widely used in tea cultivation to improve quality by modulating various metabolic pathways in tea plants. However, the differential sensitivity of specific metabolites and the cultivar-dependent responses to shading are not yet fully understood. This study examined the impact of shading on the chemical composition and transcriptional profiles of cv. Longjing 43 and cv. Yabukita. Among the main quality-related compounds, flavonol glycosides were highly responsive to shading, while catechins displayed distinct cultivar-specific responses. KEGG enrichment analysis revealed that flavonoid biosynthesis was the key secondary metabolic difference between cv. Longjing and cv. Yabukita plants under the sunlight, and shading regulated flavonoid biosynthetic pathways in both cultivars. The genes such as ANTHOCYANIDIN REDUCTASE (CsANR) and ANTHOCYANIDIN SYNTHASE (CsANS) were less sensitive to shade in cv. Longjing 43 than cv. Yabukita, leading to relatively higher levels of epi-type catechins in the shade-treated cv. Longjing 43 sample. Additionally, the UVR8-mediated light signaling pathway demonstrated cultivar-specific expression patterns, although the functional roles of key signaling proteins were conserved across both cultivars. The insights into the chemical and molecular responses of tea plants to shading deepen our understanding of the mechanisms driving the cultivar-dependent behaviors of flavonoids, which offers valuable applications for maintaining consistent matcha quality and informing breeding programs of matcha.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.