Vibrio cholerae and Vibrio paracholerae bacteraemia strains encompass lineages that share resistome and virulome profiles.

IF 2.5 4区医学 Q2 PARASITOLOGY Memorias do Instituto Oswaldo Cruz Pub Date : 2025-02-03 eCollection Date: 2025-01-01 DOI:10.1590/0074-02760240159

Sergio Mascarenhas Morgado, Erica Lourenço da Fonseca, Ana Carolina Paulo Vicente

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Abstract

Background: Non-O1 and non-O139 Vibrio cholerae (NOVC) that cause bacteraemia have attracted the attention of the public health community around the world, mainly due to the prospect of outbreaks and the way to treat such infections.

Objectives: To identify V. cholerae lineages and their antibiotic resistance and virulence factors associated with bacteraemia.

Methods: Vibrio cholerae genomes associated with strains isolated from blood were retrieved and subjected to core genome-based phylogenomic analysis with Roary. The virulome and resistome were searched with abricate using the VFDB and CARD databases.

Findings: Analysis showed that, in addition to V. cholerae, Vibrio paracholerae also causes bacteraemia. The NOVC group was highly diverse, although genomes from different countries were related. Most bacteraemic Vibrios came from countries not affected by epidemic/endemic cholera. The NOVCs virulome presented factors, such as type III and VI secretion systems, HapA, HlyA, RTX, and TLH. Importantly, no resistance to third-generation cephalosporin has been identified in the resistome of NOVCs.

Main conclusions: The presence of multiple NOVC lineages that cause bacteraemia in different parts of the world shows that there is no geographic and socioeconomic restriction for these cases. Therefore, healthcare systems need to be aware of this uncommon but deadly Vibrio infection.

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霍乱弧菌和副霍乱弧菌菌血症菌株包括具有相同抗性组和病毒组谱的谱系。

背景：引起菌血症的非o1型和非o139型霍乱弧菌（NOVC）引起了全世界公共卫生界的关注，主要是由于其暴发的前景和治疗这类感染的方法。目的：鉴定霍乱弧菌谱系及其与菌血症相关的抗生素耐药性和毒力因子。方法：提取血源霍乱弧菌相关基因组，采用Roary软件进行核心基因组系统基因组分析。利用VFDB和CARD数据库对病毒组和抗性组进行abricate检索。结果：分析表明，除霍乱弧菌外，副霍乱弧菌也可引起菌血症。尽管来自不同国家的基因组是相关的，但NOVC组是高度多样化的。大多数致菌弧菌来自未受流行/地方性霍乱影响的国家。NOVCs病毒组表现为III型和VI型分泌系统、HapA、HlyA、RTX、TLH等因子。重要的是，在novc的抵抗组中未发现对第三代头孢菌素的耐药性。主要结论：在世界不同地区存在多种引起菌血症的NOVC谱系，表明这些病例没有地理和社会经济限制。因此，卫生保健系统需要意识到这种不常见但致命的弧菌感染。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.