Effects of monosaccharides and protein in extracellular polymeric substances of Klebsiella aerogenes on crystalline forms of CaCO3 crystals

IF 4.7 1区 材料科学 Q1 METALLURGY & METALLURGICAL ENGINEERING Transactions of Nonferrous Metals Society of China Pub Date : 2024-12-01 DOI:10.1016/S1003-6326(24)66660-4
Nan HU , Ai-shu LI , Huang YU , Yu-long LIU , Hui ZHANG , Zhi-hui YANG , Guang-yue LI , De-xin DING
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Abstract

A strain of Klebsiella aerogenes (K. aerogenes) capable of decomposing calcium acetate for CaCO3 biomineralization was screened, and the optimum conditions for producing the maximum mass of CaCO3 crystals induced by Klebsiella aerogenes were found to be 0.25 mol/L calcium acetate, 4% inoculum level, and pH 7. Following that, the monosaccharides and proteins contained in the extracellular polymeric substances (EPS) of Klebsiella aerogenes were identified. The effects of additional identified monosaccharide and proteins on the crystalline form conversion of CaCO3 crystals were investigated, and the additional monosaccharides were found to promote the crystalline form conversion of CaCO3 crystals from vaterite to calcite. Specifically, adding 1.00 g/L glucuronic acid was observed to enhance the conversion of crystalline phases of CaCO3 crystals to calcite completely after 5 d. Meanwhile, additional catalase was observed to contribute to the regular morphology of CaCO3 crystals without affecting their crystalline phases. The results indicate that the additional glucuronic acid influences the crystalline forms of CaCO3 crystals significantly.
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产气克雷伯菌胞外聚合物质中单糖和蛋白质对CaCO3晶体形态的影响
筛选了一株能够分解醋酸钙进行CaCO3生物矿化的产气克雷伯菌,发现产气克雷伯菌诱导CaCO3晶体最大质量的最佳条件为:0.25 mol/L醋酸钙、4%接种量、pH 7。随后,对产气克雷伯菌胞外聚合物质(EPS)中的单糖和蛋白质进行了鉴定。研究了添加鉴定单糖和蛋白质对CaCO3晶体晶型转化的影响,发现添加单糖能促进CaCO3晶体由水晶石向方解石的晶型转化。其中,添加1.00 g/L葡萄糖醛酸可以促进CaCO3晶体在5 d后完全转化为方解石。同时,添加过氧化氢酶可以在不影响CaCO3晶体相的情况下促进CaCO3晶体形态的规则化。结果表明,葡萄糖醛酸的加入对CaCO3晶体的结晶形态有显著影响。
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来源期刊
CiteScore
7.40
自引率
17.80%
发文量
8456
审稿时长
3.6 months
期刊介绍: The Transactions of Nonferrous Metals Society of China (Trans. Nonferrous Met. Soc. China), founded in 1991 and sponsored by The Nonferrous Metals Society of China, is published monthly now and mainly contains reports of original research which reflect the new progresses in the field of nonferrous metals science and technology, including mineral processing, extraction metallurgy, metallic materials and heat treatments, metal working, physical metallurgy, powder metallurgy, with the emphasis on fundamental science. It is the unique preeminent publication in English for scientists, engineers, under/post-graduates on the field of nonferrous metals industry. This journal is covered by many famous abstract/index systems and databases such as SCI Expanded, Ei Compendex Plus, INSPEC, CA, METADEX, AJ and JICST.
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