Extended-spectrum beta-lactamase-producing Escherichia coli through the poultry production chain in Argentina

IF 2.7 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI:10.1016/j.vetmic.2025.110421

Juliana González , María Soledad Ríos , Luciana Hernandez , María Laura Chiapparrone , María Belén Riccio , Claudio Cacciato , Rocío Colello , Ana Victoria Bustamante , Andrea Mariel Sanso

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Abstract

The presence of extended-spectrum β-lactamases-producing Escherichia coli (ESBL-EC) in food production systems is a public health problem, since they can be transmitted to humans. The aims of this study were to isolate and characterize ESBL-EC from poultry farms located in Argentina. Resistances to β-lactams (first-, third-, and fourth-generation cephalosporins, penicillin), tetracyclines, quinolones, sulfonamides, phosphonates and aminoglycosides were found among the 40 ESBL-EC isolates. In addition, a wide range of antimicrobial resistance profiles were identified. The most frequent accompanying resistance to non-β-lactam antibiotics was tetracycline (97.5 %), followed by ciprofloxacin (82.5 %). All ESBL-EC isolates were multidrug resistant, and were grouped into four bla_CTX-M groups: bla_CTX-M-2 (75 %), followed by bla_CTX-M-1, bla_CTX-M-1/bla_CTX-M-2, and bla_CTX-M-2/bla_CTX-M-8. Of the 40 ESBL-EC isolates, 31 carried genes encoding integrase, 28 were positive for intI1- qacEΔ1- sul1, three for intI1, three were identified as Verocytotoxin-producing E. coli (VTEC) and most of the studied isolates belonged to the phylogroup E. This study reports the presence of CTX-M in E. coli obtained from poultry production in Argentina and that CTX-M-2 was the main mechanism responsible for third generation cephalosporins resistance. Also, showed that apparently healthy chickens in Argentina harbor commensal multidrug-resistant (MDR) ESBL-EC, with resistances to antibiotics of critical importance for human and veterinary medicine.

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阿根廷家禽生产链中产生广谱β -内酰胺酶的大肠杆菌

在食品生产系统中存在广谱产β-内酰胺酶大肠杆菌（ESBL-EC）是一个公共卫生问题，因为它们可以传播给人类。本研究的目的是从阿根廷的家禽养殖场分离和鉴定ESBL-EC。40株esblc - ec菌株对β-内酰胺类（第一代、第三代和第四代头孢菌素、青霉素）、四环素类、喹诺酮类、磺胺类、膦酸盐和氨基糖苷类耐药。此外，还确定了广泛的抗菌素耐药概况。对非β-内酰胺类抗生素耐药最多的是四环素（97.5 %），其次是环丙沙星（82.5 %）。所有ESBL-EC分离株均为多重耐药菌株，分为4个blaCTX-M组：blaCTX-M-2（75% %）、blaCTX-M-1、blaCTX-M-1/blaCTX-M-2和blaCTX-M-2/blaCTX-M-8。40株esblc - ec分离株中，31株携带整合酶编码基因，28株intI1- qacEΔ1- sul1阳性，3株intI1阳性，3株鉴定为产维胞毒素大肠杆菌（VTEC），大多数研究分离株属于e种群。本研究报道了阿根廷家禽生产的大肠杆菌中存在CTX-M， CTX-M-2是导致第三代头孢菌素耐药的主要机制。结果还表明，阿根廷明显健康的鸡体内含有共生多药耐药（MDR） ESBL-EC，对人类和兽药至关重要的抗生素具有耐药性。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.