Characterization of modified rape straw biochar in immobilizing Aspergillus sydowii W1 pellets and evaluation on its role as a novel composite for di(2-ethylhexyl) phthalate degradation

Abstract

Di(2-ethylhexyl) phthalate (DEHP) is one of the most widely used plasticizers, which has harmful biological effects and poses a serious threat to ecological environments and human health. In this study, a novel strain Aspergillus sydowii W1 was reported with DEHP degradation ability. Under the optimal conditions of 35°C and pH 6.0, strain W1 degraded 68.48 % of 50 mg/L DEHP within 120 h, while the biochar immobilized W1 can enhance the removal efficiency by 15.33 %. The immobilized W1 also showed excellent performance in DEHP polluted wastewater with concentration of 50 mg/L, and its removal rate reached 85.72 % within 144 h. Interestingly, the fermented broth of strain W1 has the activity of hydrolyzing DEHP, and the highest value of crude enzyme activity was at 35°C and pH 8.5. In addition, nine metabolic products of DEHP degraded by strain W1 were identified by HPLC-MS/MS and GC-MS. In combination with these intermediates and related enzymatic analysis, two possible catabolism pathways of DEHP degradation by strain W1 were concluded. This study confirmed that immobilized W1 is an effective composite for removing DEHP in water environment and also strengthened our understanding on the DEHP degradation process of A. sydowii.