Systematic characterization of the bZIP transcription factor family of Japanese plum (Prunus salicina Lindl.) and their potential role in phenolic compound biosynthesis

IF 4.2 2区农林科学 Q1 HORTICULTURE Scientia Horticulturae Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI:10.1016/j.scienta.2025.113962

Javiera Ibáñez , Benjamín Battistoni , Arnau Fiol , Andrew P. Dare , Paulina Ballesta , Sebastián Ahumada , Lee A. Meisel , Andrew Allan , Richard Espley , Igor Pacheco

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Abstract

The basic leucine zipper (bZIP) transcription factors are essential in several plant developmental and physiological processes. The gene family encoding these proteins has yet to be studied in Japanese plum (Prunus salicina Lindl.), a species characterized by a high phenolic content in their fruits. In this work, we analyzed the Prunus salicina reference genome, identifying 60 genes coding for bZIP proteins. According to the predicted amino acid sequences, the PsabZIPs were distributed in 12 groups. We also studied the distribution and duplication events of these bZIP genes in the 'Sanyueli' reference genome. Their orthologues were analyzed in three additional Prunus species, with peach (Prunus persica (L.) Batsch) identified as the species containing the highest number of bZIP-coding genes. An RNA-seq analysis of fruits at different developmental stages in two Japanese plum cultivars allowed us to detect 53 expressed bZIP-coding genes, suggesting a role in fruit development. Through a correlation analysis between their expression and nine phenolic compounds, we detected 29 genes with a significant association (p-value <0.001) with the biosynthesis of these compounds. These results suggest the potential role of bZIP transcription factors as regulators of phenolic compound biosynthesis in Japanese plum.

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日本李bZIP转录因子家族的系统表征及其在酚类化合物生物合成中的潜在作用

亮氨酸拉链（bZIP）转录因子在植物发育和生理过程中起重要作用。编码这些蛋白的基因家族尚未在果实中酚含量高的日本李（Prunus salicina Lindl.）中进行研究。在这项工作中，我们分析了水杨树参考基因组，鉴定了60个编码bZIP蛋白的基因。根据预测的氨基酸序列，psabzip分布在12组中。我们还研究了这些bZIP基因在“三月丽”参考基因组中的分布和重复事件。对另外3种李属植物与桃树（Prunus persica， L.）的同源性进行了分析。Batsch)被鉴定为含有最多bzip编码基因的物种。通过对两个日本李品种不同发育阶段果实的RNA-seq分析，我们检测到53个表达的bzip编码基因，这表明bzip编码基因在果实发育中起作用。通过对它们的表达与9种酚类化合物之间的相关性分析，我们检测到29个基因与这些化合物的生物合成有显著关联（p值<；0.001）。这些结果提示bZIP转录因子可能在日梅酚类化合物的生物合成中起调节作用。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.