Electrochemical study of oxidized hyaluronic acid grafted with aniline tetramer Bletilla striata polysaccharide hydrogel wound dressings.

Abstract

Bletilla striata polysaccharide, also known as Bletilla gum, is a water-soluble polymeric viscous polysaccharide with antimicrobial and coagulation-promoting activities. This study used Bletilla striata polysaccharide as the base material and crosslinked it with an electroactive carrier material to prepare a hydrogel with both conductivity and bioactivity. Specifically, hydroxypropyl chitosan with good bioactivity was used as a cross-linking agent. Through Schiff base reaction, oxidized hyaluronic acid grafted with aniline tetramer copolymer and oxidized Bletilla striata polysaccharide were crosslinked to prepare conductive hydrogels, and their properties were characterized. Comparative results indicate that the oxidized Bletilla striata polysaccharide-hyaluronic acid conductive hydrogel (HP/OB-OT4) prepared with 20% grafted conductive polymer aniline tetramer content exhibits good conductivity, with an electrical conductivity reaching 0.32 ± 0.013 mS/cm, meeting the requirements for microcurrent stimulation. It also shows a degradation rate of 58.46 ± 1.96% and possesses good antibacterial properties and biocompatibility, demonstrating potential for application in biomedical material fields such as wound dressings.