Soft Tissue Augmentation by Electrospun Membranes embedded With Nano-Hydroxyapatite: Histologic and Volumetric Analyses

IF 3.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE International dental journal Pub Date : 2025-02-05 DOI:10.1016/j.identj.2025.01.005

Yu Zhu , Zuohui Xiao , Yingxin Gu , Qiang Zhi , Zhuoli Huang , Yijie Zhang , Chunan Zhang , Shichong Qiao

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Abstract

Purpose

The aim of this study was to assess the histologic and volumetric alteration in gingival tissues following grafting with electrospinning collagen membranes blended with nanophased hydroxyapatite (nHA) on the buccal side of canine teeth.

Methods

Four adult beagle dogs underwent hemisecting of the mandibular premolars, followed by the extraction of the mesial roots. Soft tissue augmentations were randomly conducted with collagen membranes composed of poly (L-Lactic-co-caprolactone) and porcine fibrinogen (PLCL/F-Fg), PLCL/F-Fg membranes blended with nHA (PLCL/F-Fg/nHA), or a sham-operated control. The animals were sacrificed at 12 weeks post-surgery and received volumetric analyses. Histological examinations at the meantime were carried out at five different depths below the crest (0, 1.5, 2.5, 3.5 and 5.5 mm).

Results

Histological examinations showed a statistically significant increase in buccal gingival thickness between PLCL/F-Fg/20%nHA and PLCL/F-Fg (3.82 ± 1.56mm vs 1.78 ± 0.20mm (at 3.5 mm); 4.23 ± 0.63mm vs 1.80 ± 0.58mm (at 5.5 mm)) as well as between PLCL/F-Fg/20%nHA and PLCL/F-Fg/10%nHA (4.23 ± 0.63mm vs 1.97 ± 0.73mm (at 5.5 mm)). Volumetric analyses showed a statistically significant decrease in volume reduction between PLCL/F-Fg/20%nHA and PLCL/F-Fg (4.46 ± 2.75mm³ vs 10.27 ± 4.77mm³) as well as between PLCL/F-Fg/20%nHA and PLCL/F-Fg/10%nHA (4.46 ± 2.75mm³ vs 10.57 ± 2.88mm³).

Conclusion

PLCL/F-Fg/20%nHA membranes demonstrated effectiveness in the gingival thickness augmentation on the buccal side of canine teeth at 12 weeks postsurgery. The electrospinning collagen membranes blended with nHA rendered an alternative treatment for soft tissue volume augmentations at the pontic site.

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嵌入纳米羟基磷灰石的电纺丝膜增强软组织：组织学和体积分析。

目的：观察静电纺丝胶原膜与纳米羟基磷灰石（nHA）混合膜在牙颊侧移植后牙龈组织的组织学和体积变化。方法：对4只成年比格犬进行下颌前磨牙半切开术，然后拔出近中牙根。软组织增强随机使用由聚（l -乳酸-co-己内酯）和猪纤维蛋白原（PLCL/F-Fg）组成的胶原膜，PLCL/F-Fg混合nHA膜（PLCL/F-Fg/nHA）或假手术对照组进行。术后12周处死动物并进行体积分析。同时在嵴以下5个不同深度（0、1.5、2.5、3.5和5.5 mm）进行组织学检查。结果：组织学检查显示，PLCL/F-Fg/20%nHA组与PLCL/F-Fg组的颊龈厚度增加有统计学意义(3.82±1.56mm vs 1.78±0.20mm（在3.5 mm处）；（4.23±0.63mm vs 1.80±0.58mm (5.5 mm)）以及PLCL/F-Fg/20%nHA和PLCL/F-Fg/10%nHA之间（4.23±0.63mm vs 1.97±0.73mm (5.5 mm)）。体积分析显示，PLCL/F-Fg/20%nHA和PLCL/F-Fg之间的体积减少（4.46±2.75mm3 vs 10.27±4.77mm3）以及PLCL/F-Fg/20%nHA和PLCL/F-Fg/10%nHA之间的体积减少（4.46±2.75mm3 vs 10.57±2.88mm3）具有统计学意义。结论：PLCL/F-Fg/20%nHA膜在术后12周对犬牙颊侧牙龈增厚有较好的效果。与nHA混合的电纺丝胶原膜为桥桥部位的软组织体积增加提供了另一种治疗方法。

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.