Hua Yu Xiao Zheng decoction induces ectopic endometrial stromal cell senescence via inhibiting the PI3K/AKT signaling.

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-30 DOI:10.1016/j.tice.2025.102763

Yuan Xu, Yuhui Li, Jingyong Zhang, Pingping Cai

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Abstract

Background: Our purpose is to explore the influence of Hua Yu Xiao Zheng (HYXZ) decoction on the senescence of ectopic endometrial stromal cells (ESCs) in endometriosis as well as its relevant mechanisms.

Methods: The proliferation, apoptosis, migration, and invasion of primary ectopic ESCs was assessed utilizing EdU assay, flow cytometry, and transwell assays. Moreover, the effects of HYXZ decoction on oxidative stress were evaluated using DCFH-DA probe and via measuring MDA, GSH, SOD and GPx levels. The function of HYXZ decoction on cell senescence were explored through utilizing SA-β-gal staining and measuring the cumulative population doubling level and the average telomere length. The protein expression was measured using western blotting analysis. Endometriosis in rats was surgically induced, and the efficacy and mechanism of HYXZ decoction were determined in vivo.

Results: HYXZ decoction inhibited the growth, migration, and invasion of ectopic ESCs, but induced oxidative stress and senescence. HYXZ decoction inhibited PI3K/AKT signaling in ectopic ESCs. PI3K-AKT signaling pathway activator (740Y-P) significantly reversed the inhibitory effects of HYXZ decoction on ectopic ESCs. In rats with endometriosis, oral administration of HYXZ decoction inhibited lesion volume alone with the increased oxidative stress and cell senescence, as well as the decreased PI3K/AKT activity.

Conclusion: HYXZ decoction might repress the growth and migration of ectopic ESCs, and induce oxidative stress and senescence through suppressing PI3K/AKT signaling. This finding indicates that HYXZ decoction may be a potential therapeutic drug against endometriosis.

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化瘀消正汤通过抑制PI3K/AKT信号通路诱导异位子宫内膜间质细胞衰老。

背景：我们的目的是探讨化瘀消正汤对子宫内膜异位症异位子宫内膜基质细胞（ESCs）衰老的影响及其相关机制。方法：采用EdU法、流式细胞术和transwell法评估原发性异位ESCs的增殖、凋亡、迁移和侵袭。此外，采用DCFH-DA探针和测定MDA、GSH、SOD和GPx水平，评价HYXZ汤对氧化应激的影响。采用SA-β-gal染色法，测定细胞累积倍增水平和平均端粒长度，探讨HYXZ汤对细胞衰老的作用。western blotting检测蛋白表达。采用手术诱导大鼠子宫内膜异位症的方法，在体内观察合心汤的治疗效果及作用机制。结果：HYXZ汤抑制异位ESCs的生长、迁移和侵袭，但诱导氧化应激和衰老。HYXZ汤抑制异位ESCs中PI3K/AKT信号通路。PI3K-AKT信号通路激活因子（740Y-P）显著逆转HYXZ汤对异位ESCs的抑制作用。在子宫内膜异位症大鼠中，口服HYXZ汤单独抑制病变体积，增加氧化应激和细胞衰老，降低PI3K/AKT活性。结论：HYXZ汤可能通过抑制PI3K/AKT信号通路，抑制异位ESCs的生长和迁移，诱导氧化应激和衰老。这一发现提示HYXZ汤可能是一种潜在的治疗子宫内膜异位症的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.