Omic characterisation of multi-component defences against the necrotrophic pathogen Pyrenophora tritici-repentis in wheat

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2025-02-07 DOI:10.1111/plb.13746
L. C. Ferreira, F. M. Santana, S. M. M. Scagliusi, M. Beckmann, L. A. J. Mur
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小麦抗坏死性致病菌枯黄菌多组分防御的组学特征。
谭斑病是由坏死性致病菌(Pyrenophora tritrii -repentis, Ptr)引起的,对全球小麦生产构成重大威胁。因此,需要确定新的耐药来源,并对宿主对Ptr的反应有更全面的机制理解。在此,我们描述了来自多亲本高代杂交(MAGIC)群体的toxa阳性Ptr菌株与亲本小麦品系之间的相互作用。通过组织学、代谢组学和转录组学方法确定并表征了对Ptr攻击表现出中度抗性(“Robigus”)或易感性(“Hereward”)的基因型。组织学调查表明,罗比丘的突出的乳头为基础的防御。转录组学分析可以将其与屏障相关基因的表达联系起来,即肌动蛋白聚合、胼胝质沉积、囊泡运输和纤维素合成。用细胞松弛素E抑制肌动蛋白聚合增加了病变数量,但没有增加病变的生长,提示其他防御机制的部署。这些可能受到生长素的影响,因为它的外源性应用加剧了症状的发展。在herward的转录组学和代谢组学分析中,Ptr挑战提示初级代谢发生变化,影响糖酵解、TCA循环和γ-氨基丁酸(GABA)分流。水杨酸(SA)相关基因(包括NPR1和WRKY33)的激活是herward特有的,外源SA增加了两种基因型对Ptr的易感性。这项研究表明,屏障防御可以有效地对抗Ptr,以及缺乏SA等易感因子或适当处理IAA。这些发现为提高小麦对Ptr的抗性提供了潜在的途径。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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