Shu-Hui Men , Jia-Yun Xu , Zhen-Guang Yan , Jin-Zhe Du , Ming-Rui Li , Zi-Ye Wang , Chen Liu , Xin Zheng
{"title":"Regulation roles of dre-miR-187 in neurological damage by low concentration toluene in zebrafish","authors":"Shu-Hui Men , Jia-Yun Xu , Zhen-Guang Yan , Jin-Zhe Du , Ming-Rui Li , Zi-Ye Wang , Chen Liu , Xin Zheng","doi":"10.1016/j.jclepro.2025.144973","DOIUrl":null,"url":null,"abstract":"<div><div>Toluene is a common organic pollutant in ambient water environment, and its potential toxicogenic mechanism remains unclear. In this study, we explored toxicity mechanisms of toluene on zebrafish and verified that toluene exposure at criterion concentration levels altered the blood-brain barrier (BBB) permeability, and 19 lncRNAs and 10 miRNAs associated with the BBB function showed significant differences. In addition, we confirmed that <em>dre-miR-187</em> binds complementarily to ABCC4 3′UTR, thereby inhibiting expression and affecting normal BBB function. By exploring the regulatory mechanism of ncRNA under toluene stress, we provided a new idea for studying neurotoxicity of toluene and its regulatory mechanism, and for the first time found that low concentration of toluene can disrupt BBB function in zebrafish.</div></div>","PeriodicalId":349,"journal":{"name":"Journal of Cleaner Production","volume":"493 ","pages":"Article 144973"},"PeriodicalIF":10.0000,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cleaner Production","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0959652625003233","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Toluene is a common organic pollutant in ambient water environment, and its potential toxicogenic mechanism remains unclear. In this study, we explored toxicity mechanisms of toluene on zebrafish and verified that toluene exposure at criterion concentration levels altered the blood-brain barrier (BBB) permeability, and 19 lncRNAs and 10 miRNAs associated with the BBB function showed significant differences. In addition, we confirmed that dre-miR-187 binds complementarily to ABCC4 3′UTR, thereby inhibiting expression and affecting normal BBB function. By exploring the regulatory mechanism of ncRNA under toluene stress, we provided a new idea for studying neurotoxicity of toluene and its regulatory mechanism, and for the first time found that low concentration of toluene can disrupt BBB function in zebrafish.
甲苯是环境水环境中常见的有机污染物，其潜在的毒性机制尚不清楚。在本研究中，我们探索了甲苯对斑马鱼的毒性机制，并验证了标准浓度水平的甲苯暴露改变了血脑屏障（BBB）的通透性，与血脑屏障功能相关的19个lncrna和10个mirna存在显著差异。此外，我们证实了re- mir -187与ABCC4 3'UTR互补结合，从而抑制表达并影响正常血脑屏障功能。通过探索ncRNA在甲苯胁迫下的调控机制，为研究甲苯的神经毒性及其调控机制提供了新的思路，并首次发现低浓度甲苯可破坏斑马鱼血脑屏障功能。
期刊介绍：
The Journal of Cleaner Production is an international, transdisciplinary journal that addresses and discusses theoretical and practical Cleaner Production, Environmental, and Sustainability issues. It aims to help societies become more sustainable by focusing on the concept of 'Cleaner Production', which aims at preventing waste production and increasing efficiencies in energy, water, resources, and human capital use. The journal serves as a platform for corporations, governments, education institutions, regions, and societies to engage in discussions and research related to Cleaner Production, environmental, and sustainability practices.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
