An overview of biosensor advancements for detecting botulinum neurotoxins: addressing food safety and biowarfare risks.

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2025-02-05 DOI:10.1016/j.ab.2025.115801

Arzoo Saini, Neelam Yadav, Bijender Singh, Jogender Singh Rana

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引用次数: 0

Abstract

Botulinum neurotoxins (BoNTs) are lethal toxins produced by bacteria Clostridium botulinum. Ingestion of BoNTs contaminated foods causes botulism which affects individual's nervous system by blocking the release of neurotransmitters causing flaccid paralysis. This review article deciphers the comprehensive account on mechanism of action of BoNTs, pathogenicity, and various innovative analytical detection techniques of BoNTs in foods. Potential misuse of BoNT as a biowarfare agent is also a major concern. Hence, for the detection of deadly BoNTs various conventional techniques like mouse lethality bioassay (MLB), SNAP-25 assay, mouse phrenic nerve hemidiaphragm (MPN) test, non-lethal mouse flaccid paralysis assay (NFPA) and modern techniques (immunoassays, cell-based assay, nucleic acid-based methods, endopeptidase mass spectrometry assays) have been discussed. This article also provides a detailed account on biosensing technology for detecting BoNTs in foods. Moreover, future research efforts should be focused on the development of advanced new-age biosensors for automated detection and real time monitoring of botulinum neurotoxin toxicity in food. Integration of biosensors with quantum technology and lab-on-chip platforms is required for increasing their versatility and robust detection. The insights presented in the review aim towards providing future research directions and increase the vigilance against potential future threats.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.