Jing Liu , Qi Sun , Lijuan Tang , Di Lv , Yuanmei Chen , Fang Ye , Die Liu , Haixiao Liang , Chao Wang , Qi Zhang
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引用次数: 0
Abstract
Low birth weight is recognized as a risk factor for adult metabolic and cardiovascular diseases. This study investigates whether term low birth weight (TLBW) neonates, who have been exposed to unfavorable settings, demonstrate compromised lipid metabolism. A widely targeted lipidomic analysis was conducted using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) on 59 plasma samples (28 TLBW and 31 term normal birth weight (TNBW) neonates. We conducted univariate and multivariate analyses to identify differential lipids. Spearman's correlation coefficient assessed the association between lipid content at birth and Z-scores for the subsequent physical growth of enrolled children. A total of 1523 lipids in 36 subcategories across 6 major categories were detected. 269 differential lipids were discerned, with 114 up-regulated and 155 down-regulated. In the TLBW group, we observed higher levels of sphingomyelins (including SM(d18:1/16:1), SM(d18:2/23:1), SM(d18:1/22:0), and SM(d18:2/24:1)), Hex3Cer(d18:1/16:0), as well as phosphatidylcholines (PC(O-14:0_20:4) and PC(O-16:0_20:4)), and cholesterol esters (CE (20:4)). In contrast, phosphatidylethanolamines (PE) such as PE (18:2_22:1), PE (18:1_22:1), and triglyceride (TG(10:0_16:2_18:2)) were lower. The KEGG enrichment analysis revealed a consistent alteration in both sphingolipid metabolism and steroid biosynthesis. Moreover, PC(O-16:0_20:4), Hex3Cer(d18:1/16:0), and CE(20:4) exhibited positive correlations with the Z-score of height-for-age at follow-up, while PE(O-18:1_24:4) and PS(20:2_22:4) showed negative correlations with the Z-score of weight-for-age. Our findings reveal novel lipidomic differences between TLBW and TNBW neonates. The observed lipid variations at birth, including sphingomyelins and glycerophospholipids, could affect subsequent growth. Further studies are needed to validate these findings in diverse populations, address confounding factors, and investigate underlying mechanisms.
期刊介绍：
This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome.
Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.
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