Association of IL10 gene SNVs rs1800896 (A>G), rs1800871 (C>T), rs1800872 (C>A) and haplotypes with COVID-19 severity and outcome in the Brazilian population

IF 2.2 4区医学 Q3 IMMUNOLOGY Human Immunology Pub Date : 2025-03-01 Epub Date: 2025-02-10 DOI:10.1016/j.humimm.2025.111261

Janaina Nicolau de Oliveira , Caroline Yukari Motoori Fernandes , Sara Mataroli de Godoy , Wilson Frantine-Silva , Pedro Luis Candido de Souza Cassela , Guilherme Lerner Trigo , Marcell Alysson Batisti Lozovoy , Zuleica Naomi Tano , Andrea Name Colado Simão , Karen Brajão de Oliveira

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Abstract

Background

Elevated concentrations of IL-10 have been detected in coronavirus disease (COVID-19) patients and are a possible disease severity marker. Single nucleotide variants (SNVs) and their haplotypes can be associated with differences in IL-10 levels and with viral disease susceptibility.

Aim

Evaluate the associations of SNVs and their haplotypes in Brazilian patients with COVID-19 severity and outcome.

Methods

In this cross-sectional and case-control study, the patients were selected from the University Hospital of State University of Londrina (HU-UEL) (n = 367) and were subdivided into mild (n = 165), moderate (n = 72) and severe (n = 130) groups. The DNA samples of the participants were subjected to real-time PCR for the detection of rs1800896 (A>G), rs1800871 (C>T) and rs1800872 (C>A) genotypes. The haplotypes were inferred with PHASE v2.1.1.

Results

The severe cases of COVID-19 were independently associated with the GG genotype (rs1800896) (P = 0.038, OR 2.522, 95 % CI 1.053–6.038) as well as with the GCC haplotype in homozygosity (P = 0.037, OR 2.767, 95 % CI 1.065–7.191).

Conclusion

These results showed that the GG genotype of rs1800896 or the GCC haplotype are associated with COVID-19 severity in Brazilian patients.

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il - 10基因snv rs1800896 （A>G）、rs1800871 （C>T）、rs1800872 （C>A）和单倍型与巴西人群COVID-19严重程度和转归的关系

在冠状病毒病（COVID-19）患者中检测到IL-10浓度升高，可能是疾病严重程度的标志。单核苷酸变异（snv）及其单倍型可与IL-10水平差异和病毒疾病易感性相关。目的评估snv及其单倍型与巴西COVID-19严重程度和预后的关系。方法在横断面和病例对照研究中，选择伦敦州立大学附属大学医院（HU-UEL）的367例患者，再分为轻度（165例）、中度（72例）和重度（130例）组。采用实时荧光定量PCR方法检测rs1800896 （A>；G）、rs1800871 （C>；T）和rs1800872 （C>；A）基因型。单倍型通过PHASE v2.1.1进行推断。结果重症病例与GG基因型（rs1800896）（P = 0.038, OR 2.522, 95% CI 1.053 ~ 6.038）和GCC单倍型纯合度独立相关（P = 0.037, OR 2.767, 95% CI 1.065 ~ 7.191）。结论rs1800896的GG基因型或GCC单倍型与巴西患者的COVID-19严重程度相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Immunology 医学-免疫学

CiteScore

5.40

自引率

7.40%

发文量

107

审稿时长

12 days

期刊介绍： The journal''s scope includes understanding the genetic and functional mechanisms that distinguish human individuals in their immune responses to allografts, pregnancy, infections or vaccines as well as the immune responses that lead to autoimmunity, allergy or drug hypersensitivity. It also includes examining the distribution of the genes controlling these responses in populations. Research areas include: Studies of the genetics, genomics, polymorphism, evolution, and population distribution of immune-related genes Studies of the expression, structure and function of the products of immune-related genes Immunogenetics of susceptibility to infectious and autoimmune disease, and allergy The role of the immune-related genes in hematopoietic stem cell, solid organ, and vascularized composite allograft transplant Histocompatibility studies including alloantibodies, epitope definition, and T cell alloreactivity Studies of immunologic tolerance and pregnancy T cell, B cell, NK and regulatory cell functions, particularly related to subjects within the journal''s scope Pharmacogenomics and vaccine development in the context of immune-related genes Human Immunology considers immune-related genes to include those encoding classical and non-classical HLA, KIR, MIC, minor histocompatibility antigens (mHAg), immunoglobulins, TCR, BCR, proteins involved in antigen processing and presentation, complement, Fc receptors, chemokines and cytokines. Other immune-related genes may be considered. Human Immunology is also interested in bioinformatics of immune-related genes and organizational topics impacting laboratory processes, organ allocation, clinical strategies, and registries related to autoimmunity and transplantation.