Detection of a caf1 gene homolog associated with Yersinia pestis in rodent spleen samples in Lorestan Province, Iran

IF 1 Q4 GENETICS & HEREDITY Gene Reports Pub Date : 2025-02-04 DOI:10.1016/j.genrep.2025.102158

Mohammad Hassan Kayedi , Saber Esmaeili , Hossein Ahangari Cohan , Ahmad Mahmoudi , Ahmad Ghasemi , Neda Baseri , Asadollah Hosseini Chegeni , Mahdi Rohani , Ali Mohammadi , Anne Derbise , Abdolrazagh Hashemi Shahraki , Ehsan Mostafavi

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Abstract

This study aimed to investigate the presence of genes associated with Yersinia pestis, in rodents from plague reservoir environment in Lorestan Province, Iran.

In 2016 and 2017, rodents were trapped, and ectoparasites from their body surfaces were collected. DNA was extracted from the spleens of the rodents, and quantitative real-time polymerase chain reaction (qPCR) targeted the pla (pPCP1), caf1 (pMT1), and yihN genes, while conventional PCR was amplified the yihN, ymt, inv, and fur genes. IgG antibodies against the F1 capsular antigen of Y. pestis were assessed using enzyme-linked immunosorbent assay (ELISA), and specimens from the second year were cultured to isolate Y. pestis.

The most frequently captured rodent was Meriones persicus (49.59 %), followed by Mus. macedonicus (17.88 %). Of the 234 ectoparasites found, 174 were fleas (170 from Xenopsylla and 4 from Paradoxopsyllus and 60 were ticks from the Hyalomma genus. Four rodent spleen samples (one M. persicus, one Mus. macedonicus, and two Microtus spp.) tested positive for the caf1 gene homolog, but other genes associated with Y. pestis (pla, yihN, ymt, inv, and fur), IgG antibodies against the F1 antigen of Y. pestis, and culture tests were negative.

The presence of the caf1 gene, along with the absence of other Y. pestis genes and negative serology results, suggests identification of caf1 gene homolog, associated with Y. pestis in an unknown organism. Resource constraints limit our ability to conduct further tests for precise identification. Further studies should focus on additional tests to comprehensively identify the organism linked to the positive caf1 result.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.