Acclimation strategy and nitrate supplementation significantly enhanced the ammonium tolerance of Arthrospira platensis HN5

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Environmental Technology & Innovation Pub Date : 2025-05-01 Epub Date: 2025-02-07 DOI:10.1016/j.eti.2025.104076

Qing Yu , Yali Wang , Kaixuan Wang , Wenjie Tian , Xiaobin Wen , Yi Ding , Yeguang Li , Zhongjie Wang

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Abstract

The highly toxic effects of ammonium on Arthrospira hinder its utilization as a nitrogen source in Arthrospira cultivation and severely limit its application in treating high-ammonium wastewater. This study revealed that both short-term (12 d) and long-term (270 d) ammonium acclimation significantly improved the ammonium tolerance of Arthrospira platensis HN5, with biomass productivities of 0.14 and 0.11 g L^–1 d^–1, respectively, and net photosynthetic oxygen evolution rates exceeding 60 and 50 μmol O₂·mg^–1·Chla·h^–1 under a lethal ammonium concentration of 15 mM. Furthermore, the ammonium tolerance of the long-term acclimated strain exhibited heritable characteristics. Additionally, nitrate (NaNO₃) concentrations of ≥ 0.1 mM were demonstrated to effectively enhance the ammonium tolerance of A. platensis HN5 in a concentration-independent manner. This study highlighted the role of acclimation and nitrate supplementation in improving the ammonium tolerance of A. platensis HN5, offering efficient and reliable strategies for ammonium utilization in the Arthrospira industry and for treating high-ammonium wastewater using Arthrospira.

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驯化策略和添加硝酸盐显著提高了平节螺旋藻HN5对铵的耐受性

铵对节螺旋菌的高毒性作用阻碍了其作为氮源在节螺旋菌培养中的应用，严重限制了其在处理高铵废水中的应用。本研究表明，短期（12 d）和长期（270 d）铵驯化均显著提高了高原节螺HN5的铵耐量，在致死铵浓度为15 mM的条件下，其生物量生产力分别为0.14和0.11 g L-1 d - 1，净光合出氧速率分别超过60和50 μmol O2·mg-1·Chla·h-1。长期驯化菌株的耐铵性表现出遗传特性。此外，≥ 0.1 mM的硝酸根（NaNO3）能有效提高白桫椤HN5的耐铵性，且与浓度无关。本研究强调了驯化和补充硝酸盐对提高platensis HN5耐铵性的作用，为节螺旋体工业氨的利用和利用节螺旋体处理高铵废水提供了高效可靠的策略。

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来源期刊

Environmental Technology & Innovation Environmental Science-General Environmental Science

CiteScore

14.00

自引率

4.20%

发文量

435

审稿时长

74 days

期刊介绍： Environmental Technology & Innovation adopts a challenge-oriented approach to solutions by integrating natural sciences to promote a sustainable future. The journal aims to foster the creation and development of innovative products, technologies, and ideas that enhance the environment, with impacts across soil, air, water, and food in rural and urban areas. As a platform for disseminating scientific evidence for environmental protection and sustainable development, the journal emphasizes fundamental science, methodologies, tools, techniques, and policy considerations. It emphasizes the importance of science and technology in environmental benefits, including smarter, cleaner technologies for environmental protection, more efficient resource processing methods, and the evidence supporting their effectiveness.