Environmental Technology & Innovation最新文献

Effect of multi-stage inoculation on the efficiency of hyperthermophilic composting of cationic polyacrylamide-containing sludge

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-17 DOI: 10.1016/j.eti.2025.104085

Tianwen Song , Qi Meng , Jiafeng Jin , Haoshuai Li , Shanshan Duan , Wenxiang Xia , Mike Zhou

The scale of sewage treatment in China currently grows consistently, leading to a rapid increase in sewage sludge production. Cationic polyacrylamide (CPAM) is widely used to dewater sewage sludge. However, during land application and landfill processes of dewatered sludge, a considerable amount of CPAM is released into the environment, posing severe pollution risks. This study explored the effects of different inoculation strategies for Bacillus licheniformis, Calditerricola satsumensis, and Bacillus subtilis on the efficiency of hyperthermophilic composting (HTC) of CPAM-containing sludge. The results showed that multi-stage inoculation significantly enhanced the efficiency of HTC and removal rate of CPAM. Compared with the non-inoculation group, multi-stage inoculation extended the hyperthermophilic phase from 7 to 11 days, increased the maximum temperature from 82.06°C to 85.16°C, and increased the removal rate of CPAM from 72.54 % to 84.90 %. The analysis of seed germination index (GI) and fluorescence excitation-emission matrix (EEM) spectra both revealed that multi-stage inoculation promoted the decomposition and humification of organic matter. Illumina MiSeq sequencing indicated that multi-stage inoculation significantly improved the diversity and richness of the microbial communities. Firmicutes, Actinobacteriota, and Proteobacteria were the predominant bacterial phyla, while Bacillus, Calditerricola, and Sporolactobacillus were the key functional bacterial genera involved in CPAM degradation. To the best of our knowledge, this is the first study to enhance the removal efficiency of CPAM by HTC using a multi-stage inoculation approach. These findings provide valuable new strategies for the efficient treatment of CPAM-containing sludge.

{"title":"Effect of multi-stage inoculation on the efficiency of hyperthermophilic composting of cationic polyacrylamide-containing sludge","authors":"Tianwen Song , Qi Meng , Jiafeng Jin , Haoshuai Li , Shanshan Duan , Wenxiang Xia , Mike Zhou","doi":"10.1016/j.eti.2025.104085","DOIUrl":"10.1016/j.eti.2025.104085","url":null,"abstract":"<div><div>The scale of sewage treatment in China currently grows consistently, leading to a rapid increase in sewage sludge production. Cationic polyacrylamide (CPAM) is widely used to dewater sewage sludge. However, during land application and landfill processes of dewatered sludge, a considerable amount of CPAM is released into the environment, posing severe pollution risks. This study explored the effects of different inoculation strategies for <em>Bacillus licheniformis</em>, <em>Calditerricola satsumensis</em>, and <em>Bacillus subtilis</em> on the efficiency of hyperthermophilic composting (HTC) of CPAM-containing sludge. The results showed that multi-stage inoculation significantly enhanced the efficiency of HTC and removal rate of CPAM. Compared with the non-inoculation group, multi-stage inoculation extended the hyperthermophilic phase from 7 to 11 days, increased the maximum temperature from 82.06°C to 85.16°C, and increased the removal rate of CPAM from 72.54 % to 84.90 %. The analysis of seed germination index (GI) and fluorescence excitation-emission matrix (EEM) spectra both revealed that multi-stage inoculation promoted the decomposition and humification of organic matter. Illumina MiSeq sequencing indicated that multi-stage inoculation significantly improved the diversity and richness of the microbial communities. Firmicutes, Actinobacteriota, and Proteobacteria were the predominant bacterial phyla, while <em>Bacillus</em>, <em>Calditerricola</em>, and <em>Sporolactobacillus</em> were the key functional bacterial genera involved in CPAM degradation. To the best of our knowledge, this is the first study to enhance the removal efficiency of CPAM by HTC using a multi-stage inoculation approach. These findings provide valuable new strategies for the efficient treatment of CPAM-containing sludge.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104085"},"PeriodicalIF":6.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water sample stability

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-15 DOI: 10.1016/j.eti.2025.104080

Lionel PINEAU , Vanessa CORDIER

Numerous guidelines have been issued on the conditions under which water samples should be transported and stored before microbiological analysis. These recommendations are often very restrictive regarding the maximum acceptable storage time (MAST) of samples before analysis and they generate operational constraints that have significant economic and environmental consequences (e.g. samples rejected, unjustified prolongation of quarantine of a water supply point or medical device, recontrol,…). Unfortunately, the relevance of these MAST has never been demonstrated. This study investigates bacterial flora evolution in water samples according to their storage time before analysis. The first part, conducted in laboratory conditions follows the concentration of microorganisms in artificially contaminated water samples. The second part involves a retrospective analysis of real water sample results to verify whether the results varied statistically according to the sample storage time before analysis. The results of the laboratory study confirm that the microorganism concentration in the artificially contaminated samples vary by less than 25 % during the storage time. The second part of the study demonstrates that the ratio of samples belonging to the different result groups (e.g. < 1 CFU/ X mL, between 1 and 99 CFU/X mL and ≥ 100 CFU/100 mL) remains unchanged according to storage time (variation < 1 %). These findings confirm that it is possible to increase the MAST defined in standards, up to 26 h or 28 h according to the microorganism considered (instead of 12 h or 18 h) without compromising the validity of microbiological analysis. These changes should limit the operational, environmental, and economic constraints associated with the actual MAST by reducing the number of samples rejected and consecutively the number of recontrols.

{"title":"Water sample stability","authors":"Lionel PINEAU , Vanessa CORDIER","doi":"10.1016/j.eti.2025.104080","DOIUrl":"10.1016/j.eti.2025.104080","url":null,"abstract":"<div><div>Numerous guidelines have been issued on the conditions under which water samples should be transported and stored before microbiological analysis. These recommendations are often very restrictive regarding the maximum acceptable storage time (MAST) of samples before analysis and they generate operational constraints that have significant economic and environmental consequences (e.g. samples rejected, unjustified prolongation of quarantine of a water supply point or medical device, recontrol,…). Unfortunately, the relevance of these MAST has never been demonstrated. This study investigates bacterial flora evolution in water samples according to their storage time before analysis. The first part, conducted in laboratory conditions follows the concentration of microorganisms in artificially contaminated water samples. The second part involves a retrospective analysis of real water sample results to verify whether the results varied statistically according to the sample storage time before analysis. The results of the laboratory study confirm that the microorganism concentration in the artificially contaminated samples vary by less than 25 % during the storage time. The second part of the study demonstrates that the ratio of samples belonging to the different result groups (e.g. < 1 CFU/ X mL, between 1 and 99 CFU/X mL and ≥ 100 CFU/100 mL) remains unchanged according to storage time (variation < 1 %). These findings confirm that it is possible to increase the MAST defined in standards, up to 26 h or 28 h according to the microorganism considered (instead of 12 h or 18 h) without compromising the validity of microbiological analysis. These changes should limit the operational, environmental, and economic constraints associated with the actual MAST by reducing the number of samples rejected and consecutively the number of recontrols.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104080"},"PeriodicalIF":6.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A calibration approach for a passive sampler based on a polymer inclusion membrane (PIM) for in situ Zn monitoring in Catalan rivers

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-12 DOI: 10.1016/j.eti.2025.104082

Berta Alcalde, Clàudia Fontàs, Enriqueta Anticó

Time-weighted average (TWA) concentrations of Zn(II) were monitored in a mine-contaminated river in Catalonia (the Osor) by an in situ sampling point-specific kinetic calibration using a polymer inclusion membrane (PIM)-passive sampler. The PIM was composed of a polymer, cellulose triacetate (CTA), an extractant, di-(2-ethylhexyl) phosphoric acid (D2EHPA), and a plasticizer, 2-nitrophenyl octyl ether (NPOE), to allow the transport and preconcentration of free Zn species from the sampling medium to the receiving phase (0.01 mol L⁻¹ HNO₃ solution). Calibration was conducted by measuring the initial permeability under controlled laboratory conditions and by performing in situ kinetics at two different sampling points in the river. Sampling rates (R_s) of the PIM-passive sampler were also calculated, yielding results ranging from 1.7 × 10⁻¹⁰ to 3.0 × 10⁻¹⁰ m³ s⁻¹ for laboratory experiments and R_s values of 1.6 × 10⁻¹⁰ and 1.8 × 10⁻⁹ m³ s⁻¹ for in situ studies. The in situ calculated P₀ (initial permeability) was used as the calibration approach to determine the TWA concentrations of Zn in the Osor River in subsequent sampling campaigns, and values between 2 × 10⁻⁷ and 1.59 × 10⁻⁵ mol L⁻¹ of Zn were obtained. The PIM-passive sampler was then used to screen for Zn pollution in several other rivers without any evidence of pollution by this metal being found.

{"title":"A calibration approach for a passive sampler based on a polymer inclusion membrane (PIM) for in situ Zn monitoring in Catalan rivers","authors":"Berta Alcalde, Clàudia Fontàs, Enriqueta Anticó","doi":"10.1016/j.eti.2025.104082","DOIUrl":"10.1016/j.eti.2025.104082","url":null,"abstract":"<div><div>Time-weighted average (TWA) concentrations of Zn(II) were monitored in a mine-contaminated river in Catalonia (the Osor) by an in situ sampling point-specific kinetic calibration using a polymer inclusion membrane (PIM)-passive sampler. The PIM was composed of a polymer, cellulose triacetate (CTA), an extractant, di-(2-ethylhexyl) phosphoric acid (D2EHPA), and a plasticizer, 2-nitrophenyl octyl ether (NPOE), to allow the transport and preconcentration of free Zn species from the sampling medium to the receiving phase (0.01 mol L<sup>−1</sup> HNO<sub>3</sub> solution). Calibration was conducted by measuring the initial permeability under controlled laboratory conditions and by performing in situ kinetics at two different sampling points in the river. Sampling rates (R<sub>s</sub>) of the PIM-passive sampler were also calculated, yielding results ranging from 1.7 × 10<sup>−10</sup> to 3.0 × 10<sup>−10</sup> m<sup>3</sup> s<sup>−1</sup> for laboratory experiments and R<sub>s</sub> values of 1.6 × 10<sup>−10</sup> and 1.8 × 10<sup>−9</sup> m<sup>3</sup> s<sup>−1</sup> for in situ studies. The in situ calculated P<sub>0</sub> (initial permeability) was used as the calibration approach to determine the TWA concentrations of Zn in the Osor River in subsequent sampling campaigns, and values between 2 × 10<sup>−7</sup> and 1.59 × 10<sup>−5</sup> mol L<sup>−1</sup> of Zn were obtained. The PIM-passive sampler was then used to screen for Zn pollution in several other rivers without any evidence of pollution by this metal being found.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104082"},"PeriodicalIF":6.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Petaloid MoS2@MIL-88B(Fe) nanocomposite photocatalyst utilized to achieve accurate SWASV detection of Cd2+ and Pb2+ in water under low-pressure ultraviolet irradiation

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-11 DOI: 10.1016/j.eti.2025.104078

Renjie He , Liya Feng , Shaowen Chen , Shijie Zhang , Yujie Shi , Ning Liu , Gang Liu , Xiande Zhao , Guo Zhao

Humic acid (HA) is an organic compound in natural water and can be complexed with heavy metal ions (HMIs), which interferes with the detection of square wave anodic stripping voltammetry (SWASV). In this study, a petaloid MoS₂@MIL-88B(Fe) nanocomposite was synthesized via hydrothermal synthesis. Leveraging its ability to generate active species under low-pressure ultraviolet (LPUV) irradiation, a MoS₂@MIL-88B(Fe)/LPUV-based pretreatment method was proposed to detect HMIs in water by disrupting the complexation between HA and HMIs, thus restoring the SWASV signals of HMIs. The microstructure, crystal structure, surface chemical state, band gap, photogenerated charge separation and recombination rates of MoS₂@MIL-88B(Fe) were investigated, along with the degradation kinetics of HA, byproducts, and active species generated during pretreatment, to elucidate both the mechanism behind the disruption of HA-HMIs complexation and the restoration of SWASV signals. Additionally, the key pretreatment parameters, such as pH, the MoS₂ to MIL-88B(Fe) mass ratio, the photocatalyst concentration, and the photolysis time, were optimized for the choice signal restoration ratio. The proposed MoS₂@MIL-88B(Fe)/LPUV-based pretreatment method was applied to real water samples, yielding a root mean square error (RMSE) of less than 0.2 μg/L compared with that of the national standard method, confirming its authenticity and feasibility.

{"title":"Petaloid MoS2@MIL-88B(Fe) nanocomposite photocatalyst utilized to achieve accurate SWASV detection of Cd2+ and Pb2+ in water under low-pressure ultraviolet irradiation","authors":"Renjie He , Liya Feng , Shaowen Chen , Shijie Zhang , Yujie Shi , Ning Liu , Gang Liu , Xiande Zhao , Guo Zhao","doi":"10.1016/j.eti.2025.104078","DOIUrl":"10.1016/j.eti.2025.104078","url":null,"abstract":"<div><div>Humic acid (HA) is an organic compound in natural water and can be complexed with heavy metal ions (HMIs), which interferes with the detection of square wave anodic stripping voltammetry (SWASV). In this study, a petaloid MoS<sub>2</sub>@MIL-88B(Fe) nanocomposite was synthesized via hydrothermal synthesis. Leveraging its ability to generate active species under low-pressure ultraviolet (LPUV) irradiation, a MoS<sub>2</sub>@MIL-88B(Fe)/LPUV-based pretreatment method was proposed to detect HMIs in water by disrupting the complexation between HA and HMIs, thus restoring the SWASV signals of HMIs. The microstructure, crystal structure, surface chemical state, band gap, photogenerated charge separation and recombination rates of MoS<sub>2</sub>@MIL-88B(Fe) were investigated, along with the degradation kinetics of HA, byproducts, and active species generated during pretreatment, to elucidate both the mechanism behind the disruption of HA-HMIs complexation and the restoration of SWASV signals. Additionally, the key pretreatment parameters, such as pH, the MoS<sub>2</sub> to MIL-88B(Fe) mass ratio, the photocatalyst concentration, and the photolysis time, were optimized for the choice signal restoration ratio. The proposed MoS<sub>2</sub>@MIL-88B(Fe)/LPUV-based pretreatment method was applied to real water samples, yielding a root mean square error (RMSE) of less than 0.2 μg/L compared with that of the national standard method, confirming its authenticity and feasibility.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104078"},"PeriodicalIF":6.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of organic farming practices on soil health improvement of coconut farms

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-11 DOI: 10.1016/j.eti.2025.104067

Nguyen Khoi Nghia , Javad Robatjazi , Vo Duyen Thao Vy , Hüseyin Barış Tecimen , Hendra Gonsalve W. Lasar , Didier Lesueur , Shahla Hosseini Bai , Huu-Tuan Tran , Nguyen Huu Thien , Do Thanh Luan

The present study aimed to provide data on what extend of organic management (OF) improve soil health compared to conventional farms (CF). In this study, a total of 24 top soil samples (0–30 cm) were collected from Mo Cay Nam and Mo Cay Bac, Ben Tre, Vietnam, representing organic vs conventional farm soils, respectively to analyze farming systems in terms of the chemical, physical, and biological soil properties. Soil nutrients, soil microbial density, dehydrogenase enzyme activity and soil bacteria diversity were detected using atomic absorption spectrometry, serial dilution and plating; triphenyl formazan detection, and DNA extraction using Invitrogen™ and Qubit™, respectively. Our results indicated that soil bulk density was 11 % lower and soil porosity was 4 % higher, respectively at organic farms compared to the conventional farms. Meanwhile, both soil NH₄⁺ and available phosphorus contents were higher in the organic farming soils (38.9 mg kg⁻¹ and 69.0 mg kg⁻¹, respectively). Total soil bacteria and cellulose-decomposing bacteria were found to be insignificantly lower in conventional farms (CF) as compared to organic farms (OF) (6.01 log CFU g⁻¹ soil vs 6.26 log CFU g⁻¹ soil) and (3.82 log CFU g⁻¹ soil vs 4.18 log CFU g⁻¹ soil), respectively. The beta diversity of soil bacterial community, along with the bacterial orders Bacillales, Frankiales, Elsterales, Pseudomonadales, and Pedosphaerales exhibited higher with organic farming practices and dehydrogenase enzyme activity in organic farms (OF) was significantly higher (0.61 µg TPF g⁻¹ soil hour⁻¹) as compared to 0.47 µg TPF g⁻¹ soil hour⁻¹ in conventional farms (CF). This study demonstrates the benefits of organic management on soil health in coconut plantations and promotes the overall health of coconut orchards.

{"title":"Effect of organic farming practices on soil health improvement of coconut farms","authors":"Nguyen Khoi Nghia , Javad Robatjazi , Vo Duyen Thao Vy , Hüseyin Barış Tecimen , Hendra Gonsalve W. Lasar , Didier Lesueur , Shahla Hosseini Bai , Huu-Tuan Tran , Nguyen Huu Thien , Do Thanh Luan","doi":"10.1016/j.eti.2025.104067","DOIUrl":"10.1016/j.eti.2025.104067","url":null,"abstract":"<div><div>The present study aimed to provide data on what extend of organic management (OF) improve soil health compared to conventional farms (CF). In this study, a total of 24 top soil samples (0–30 cm) were collected from Mo Cay Nam and Mo Cay Bac, Ben Tre, Vietnam, representing organic vs conventional farm soils, respectively to analyze farming systems in terms of the chemical, physical, and biological soil properties. Soil nutrients, soil microbial density, dehydrogenase enzyme activity and soil bacteria diversity were detected using atomic absorption spectrometry, serial dilution and plating; triphenyl formazan detection, and DNA extraction using Invitrogen™ and Qubit™, respectively. Our results indicated that soil bulk density was 11 % lower and soil porosity was 4 % higher, respectively at organic farms compared to the conventional farms. Meanwhile, both soil NH<sub>4</sub><sup>+</sup> and available phosphorus contents were higher in the organic farming soils (38.9 mg kg<sup>−1</sup> and 69.0 mg kg<sup>−1</sup>, respectively). Total soil bacteria and cellulose-decomposing bacteria were found to be insignificantly lower in conventional farms (CF) as compared to organic farms (OF) (6.01 log CFU g<sup>−1</sup> soil vs 6.26 log CFU g<sup>−1</sup> soil) and (3.82 log CFU g<sup>−1</sup> soil vs 4.18 log CFU g<sup>−1</sup> soil), respectively. The beta diversity of soil bacterial community, along with the bacterial orders <em>Bacillales, Frankiales, Elsterales, Pseudomonadales</em>, and <em>Pedosphaerales</em> exhibited higher with organic farming practices and dehydrogenase enzyme activity in organic farms (OF) was significantly higher (0.61 µg TPF g<sup>−1</sup> soil hour<sup>−1</sup>) as compared to 0.47 µg TPF g<sup>−1</sup> soil hour<sup>−1</sup> in conventional farms (CF). This study demonstrates the benefits of organic management on soil health in coconut plantations and promotes the overall health of coconut orchards.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104067"},"PeriodicalIF":6.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grafting on rootstocks with low Cd accumulating potential: A green technology in vegetable production

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-11 DOI: 10.1016/j.eti.2025.104077

Na Sun , Fangao Zhu , Liang Sun , Guoyuan Zou , Hong Li

Recently, grafting with certain rootstock cultivars has been observed to significantly reduce Cd accumulation in above ground plant tissues. However, the mechanism remains unclear. This study comprehensively evaluated the influencing factors and their contribution in affecting Cd uptake and translocation in grafted tomato plants. One tomato scion cultivar ‘Jingcai 8’ was grafted onto two rootstock cultivars ‘Guozhen 1’ and ‘Zhenai 1’ to evaluate the grafting effect on plant Cd uptake and translocation on Cd contaminated soil. The results demonstrated that grafting with ‘Guozhen 1’ and ‘Zhenai 1’ significantly enhanced plant growth and fruit biomass. Grafted rootstocks recruited microbes, which reduced rhizosphere Cd bioavailability. However, the reduction of soil Cd bioavailability was offset by enhanced root growth which contributed to root Cd uptake. The significantly increased root biomass and fine root length in the rootstocks resulted in significantly higher root Cd uptake than in the non-grafted treatment by 6.35–20.40 %. Nevertheless, grafting with ‘Zhenai 1’ had a significantly low translocation of Cd from root to fruit (TF of 0.015) than the non-grafted treatment (TF of 0.019), which resulted in a significant reduction in fruit Cd content by 17.54 %. This was due to the effects of grafting with rootstock ‘Zhenai 1’ that reduced Cd mobility by increasing GSH and decreasing subcellular distribution in cytoplasm. Fruit Cd content was the net result of root uptake and translocation. These results showed that grafting with a suitable rootstock is a promising green and low-cost approach for the production of safe vegetables on Cd-contaminated soil.

{"title":"Grafting on rootstocks with low Cd accumulating potential: A green technology in vegetable production","authors":"Na Sun , Fangao Zhu , Liang Sun , Guoyuan Zou , Hong Li","doi":"10.1016/j.eti.2025.104077","DOIUrl":"10.1016/j.eti.2025.104077","url":null,"abstract":"<div><div>Recently, grafting with certain rootstock cultivars has been observed to significantly reduce Cd accumulation in above ground plant tissues. However, the mechanism remains unclear. This study comprehensively evaluated the influencing factors and their contribution in affecting Cd uptake and translocation in grafted tomato plants. One tomato scion cultivar ‘Jingcai 8’ was grafted onto two rootstock cultivars ‘Guozhen 1’ and ‘Zhenai 1’ to evaluate the grafting effect on plant Cd uptake and translocation on Cd contaminated soil. The results demonstrated that grafting with ‘Guozhen 1’ and ‘Zhenai 1’ significantly enhanced plant growth and fruit biomass. Grafted rootstocks recruited microbes, which reduced rhizosphere Cd bioavailability. However, the reduction of soil Cd bioavailability was offset by enhanced root growth which contributed to root Cd uptake. The significantly increased root biomass and fine root length in the rootstocks resulted in significantly higher root Cd uptake than in the non-grafted treatment by 6.35–20.40 %. Nevertheless, grafting with ‘Zhenai 1’ had a significantly low translocation of Cd from root to fruit (TF of 0.015) than the non-grafted treatment (TF of 0.019), which resulted in a significant reduction in fruit Cd content by 17.54 %. This was due to the effects of grafting with rootstock ‘Zhenai 1’ that reduced Cd mobility by increasing GSH and decreasing subcellular distribution in cytoplasm. Fruit Cd content was the net result of root uptake and translocation. These results showed that grafting with a suitable rootstock is a promising green and low-cost approach for the production of safe vegetables on Cd-contaminated soil.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104077"},"PeriodicalIF":6.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and biofilm inhibition of Helicobacter pylori using green synthesized MWCNTs/ZnO/Chitosan nanocomposites

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-11 DOI: 10.1016/j.eti.2025.104068

Saeid Fallahizadeh , Mahmood Yousefi , Ahmad Ghasemi , Seyed Abdolmohammad Sadat , Mahnaz Mohtashemi , Alieh Rezagholizade-shirvan , Mohsen Naghmachi

Helicobacter pylori, a Gram-negative bacterium, is a major cause of gastritis, peptic ulcers and gastric cancer. Its biofilm formation and antibiotic resistance in patients make it difficult for treatment. This study concerned the synthesis and antibacterial and antibiofilm evaluation of MWCNTs/ZnO/Chitosan nanocomposite against H. pylori. Green synthesis methods were used to develop the nanocomposite based on the formation of ZnO nanoparticles and the functionalization of MWCNTs. The XRD, SEM, TEM, and FTIR characteristics revealed the structural stability and the successful integration of ZnO, MWCNTs, and chitosan into the composite. The results indicated that the MIC values for ZnO nanoparticles ranged from 25 to 50 μg/mL, while those for chitosan were above 100 μg/mL. Additionally, the MWCNTs/ZnO/Chitosan nanocomposite exhibited the lowest MIC values, with 12.5 μg/mL for H. pylori clinical isolate 1 and 25 μg/mL for H. pylori clinical isolate 2 and H. pylori ATCC 43504 strains. MIC tests showed that the nanocomposite had better antibacterial activity, therefore having lower MIC values than normal antibiotics like metronidazole (MNZ) and clarithromycin (CLR) as well as its components. ZnO-induced reactive oxygen species (ROS), chitosan's bacterial membrane interaction, and MWCNTs part in improved nanoparticle distribution and mechanical biofilm disturbance all contributed to the antimicrobial mechanisms. The research points out the MWCNTs/ZnO/Chitosan nanocomposite as a hopeful solution for antibiotic-resistant H. pylori bacteria causes further in vivo experiments and medical uses rest on its synergistic antibacterial activity and ability to disrupt biofilms. These results emphasize the promise of nanotechnology in creating novel therapies to fight ongoing bacterial infections.

{"title":"Antibacterial and biofilm inhibition of Helicobacter pylori using green synthesized MWCNTs/ZnO/Chitosan nanocomposites","authors":"Saeid Fallahizadeh , Mahmood Yousefi , Ahmad Ghasemi , Seyed Abdolmohammad Sadat , Mahnaz Mohtashemi , Alieh Rezagholizade-shirvan , Mohsen Naghmachi","doi":"10.1016/j.eti.2025.104068","DOIUrl":"10.1016/j.eti.2025.104068","url":null,"abstract":"<div><div><em>Helicobacter pylori</em>, a Gram-negative bacterium, is a major cause of gastritis, peptic ulcers and gastric cancer. Its biofilm formation and antibiotic resistance in patients make it difficult for treatment. This study concerned the synthesis and antibacterial and antibiofilm evaluationof MWCNTs/ZnO/Chitosan nanocomposite against <em>H. pylori.</em> Green synthesis methods were used todevelop the nanocomposite based on the formation of ZnO nanoparticles and the functionalization of MWCNTs. The XRD, SEM, TEM, and FTIR characteristicsrevealed the structural stability and the successful integration of ZnO, MWCNTs, and chitosan into the composite. The results indicated that the MIC values for ZnO nanoparticles ranged from 25 to 50 μg/mL, while those for chitosan were above 100 μg/mL. Additionally, the MWCNTs/ZnO/Chitosan nanocomposite exhibited the lowest MIC values, with 12.5 μg/mL for <em>H. pylori</em> clinical isolate 1 and 25 μg/mL for <em>H. pylori</em> clinical isolate 2 and <em>H. pylori</em> ATCC 43504 strains. MIC tests showed that the nanocomposite had better antibacterial activity, therefore having lower MIC values than normal antibiotics like metronidazole (MNZ) and clarithromycin (CLR) as well as its components. ZnO-induced reactive oxygen species (ROS), chitosan's bacterial membrane interaction, and MWCNTs part in improved nanoparticle distribution and mechanical biofilm disturbance all contributed to the antimicrobial mechanisms. The research points out the MWCNTs/ZnO/Chitosan nanocomposite as a hopeful solution for antibiotic-resistant <em>H. pylori</em> bacteria causes further in vivo experiments and medical uses rest on its synergistic antibacterial activity and ability to disrupt biofilms. These results emphasize the promise of nanotechnology in creating novel therapies to fight ongoing bacterial infections.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104068"},"PeriodicalIF":6.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile synthesis of carbon nitride nanotube confined nano Fe0 for boosting activation of peroxymonosulfate towards tetracycline removal

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-09 DOI: 10.1016/j.eti.2025.104079

Yajun Ji , Feiya Xu , Kun Fang , Huiyun Liu , Xiaofang Pan , Zihe Jin , Lingyun Zheng , Lele Wang

The nano-sized zero-valent iron (Fe⁰) exhibits excellent activity for organic contaminant remediation by activating peroxymonosulfate (PMS). Its catalytic performance, however, was restricted due to its susceptibility towards oxidation and agglomeration. Thus, carbon nitride nanotube embedded nano Fe⁰ catalysts (Fe⁰@NC) with various Fe contents were synthesized to simultaneously overcome the drawbacks. Fe⁰@NC-10 showed high specific surface area (S_BET, 118.87 m²·g⁻¹), stable crystal structure, plentiful Fe- and N-containing active sites. Under the optimal conditions (0.05 g·L⁻¹ Fe⁰@NC-10 and 0.15 g·L⁻¹ PMS), over 86 % tetracycline (TC) could be removed after 5 min, possessing a rate constant (K_obs) value as high as 1.81 min⁻¹. The constructed Fe⁰@NC-10/PMS system also showed prominent performance even at different solution pH values or with coexisting ions. Moreover, Fe⁰@NC-10 exhibited outstanding performance in the continuous degradation experiment. It was Fe⁰, Fe–N_x and graphitic nitrogen in Fe⁰@NC-10 that activated PMS to produce ferryl Fe-oxo species (Fe^IV=O) and ¹O₂, which collectively resulted in the removal of TC. Sixteen intermediate products were detected during TC degradation, which showed lower toxicity. This study provides a simple strategy for synthesizing an active and stable Fe⁰ nano-catalyst for TC removal by activating PMS.

{"title":"Facile synthesis of carbon nitride nanotube confined nano Fe0 for boosting activation of peroxymonosulfate towards tetracycline removal","authors":"Yajun Ji , Feiya Xu , Kun Fang , Huiyun Liu , Xiaofang Pan , Zihe Jin , Lingyun Zheng , Lele Wang","doi":"10.1016/j.eti.2025.104079","DOIUrl":"10.1016/j.eti.2025.104079","url":null,"abstract":"<div><div>The nano-sized zero-valent iron (Fe<sup>0</sup>) exhibits excellent activity for organic contaminant remediation by activating peroxymonosulfate (PMS). Its catalytic performance, however, was restricted due to its susceptibility towards oxidation and agglomeration. Thus, carbon nitride nanotube embedded nano Fe<sup>0</sup> catalysts (Fe<sup>0</sup>@NC) with various Fe contents were synthesized to simultaneously overcome the drawbacks. Fe<sup>0</sup>@NC-10 showed high specific surface area (S<sub>BET,</sub> 118.87 m<sup>2</sup>·g<sup>−1</sup>), stable crystal structure, plentiful Fe- and N-containing active sites. Under the optimal conditions (0.05 g·L<sup>−1</sup> Fe<sup>0</sup>@NC-10 and 0.15 g·L<sup>−1</sup> PMS), over 86 % tetracycline (TC) could be removed after 5 min, possessing a rate constant (<em>K</em><sub>obs</sub>) value as high as 1.81 min<sup>−1</sup>. The constructed Fe<sup>0</sup>@NC-10/PMS system also showed prominent performance even at different solution pH values or with coexisting ions. Moreover, Fe<sup>0</sup>@NC-10 exhibited outstanding performance in the continuous degradation experiment. It was Fe<sup>0</sup>, Fe–N<sub>x</sub> and graphitic nitrogen in Fe<sup>0</sup>@NC-10 that activated PMS to produce ferryl Fe-oxo species (Fe<sup>IV</sup>=O) and <sup>1</sup>O<sub>2</sub>, which collectively resulted in the removal of TC. Sixteen intermediate products were detected during TC degradation, which showed lower toxicity. This study provides a simple strategy for synthesizing an active and stable Fe<sup>0</sup> nano-catalyst for TC removal by activating PMS.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104079"},"PeriodicalIF":6.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acclimation strategy and nitrate supplementation significantly enhanced the ammonium tolerance of Arthrospira platensis HN5

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-07 DOI: 10.1016/j.eti.2025.104076

Qing Yu , Yali Wang , Kaixuan Wang , Wenjie Tian , Xiaobin Wen , Yi Ding , Yeguang Li , Zhongjie Wang

The highly toxic effects of ammonium on Arthrospira hinder its utilization as a nitrogen source in Arthrospira cultivation and severely limit its application in treating high-ammonium wastewater. This study revealed that both short-term (12 d) and long-term (270 d) ammonium acclimation significantly improved the ammonium tolerance of Arthrospira platensis HN5, with biomass productivities of 0.14 and 0.11 g L^–1 d^–1, respectively, and net photosynthetic oxygen evolution rates exceeding 60 and 50 μmol O₂·mg^–1·Chla·h^–1 under a lethal ammonium concentration of 15 mM. Furthermore, the ammonium tolerance of the long-term acclimated strain exhibited heritable characteristics. Additionally, nitrate (NaNO₃) concentrations of ≥ 0.1 mM were demonstrated to effectively enhance the ammonium tolerance of A. platensis HN5 in a concentration-independent manner. This study highlighted the role of acclimation and nitrate supplementation in improving the ammonium tolerance of A. platensis HN5, offering efficient and reliable strategies for ammonium utilization in the Arthrospira industry and for treating high-ammonium wastewater using Arthrospira.

{"title":"Acclimation strategy and nitrate supplementation significantly enhanced the ammonium tolerance of Arthrospira platensis HN5","authors":"Qing Yu , Yali Wang , Kaixuan Wang , Wenjie Tian , Xiaobin Wen , Yi Ding , Yeguang Li , Zhongjie Wang","doi":"10.1016/j.eti.2025.104076","DOIUrl":"10.1016/j.eti.2025.104076","url":null,"abstract":"<div><div>The highly toxic effects of ammonium on <em>Arthrospira</em> hinder its utilization as a nitrogen source in <em>Arthrospira</em> cultivation and severely limit its application in treating high-ammonium wastewater. This study revealed that both short-term (12 d) and long-term (270 d) ammonium acclimation significantly improved the ammonium tolerance of <em>Arthrospira platensis</em> HN5, with biomass productivities of 0.14 and 0.11 g L<sup>–1</sup> d<sup>–1</sup>, respectively, and net photosynthetic oxygen evolution rates exceeding 60 and 50 μmol O<sub>2</sub>·mg<sup>–1</sup>·Chla·h<sup>–1</sup> under a lethal ammonium concentration of 15 mM. Furthermore, the ammonium tolerance of the long-term acclimated strain exhibited heritable characteristics. Additionally, nitrate (NaNO<sub>3</sub>) concentrations of ≥ 0.1 mM were demonstrated to effectively enhance the ammonium tolerance of <em>A. platensis</em> HN5 in a concentration-independent manner. This study highlighted the role of acclimation and nitrate supplementation in improving the ammonium tolerance of <em>A. platensis</em> HN5, offering efficient and reliable strategies for ammonium utilization in the <em>Arthrospira</em> industry and for treating high-ammonium wastewater using <em>Arthrospira</em>.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104076"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic effect of extracellular polysaccharide-producing salt-tolerant bacteria and biochar promotes grape growth under saline-alkaline stress

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-07 DOI: 10.1016/j.eti.2025.104070

Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du

Grapes (Vitis vinifera) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected Bacillus subtilis B4 and Pseudomonas resinovorans B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional B. subtilis, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.

{"title":"The synergistic effect of extracellular polysaccharide-producing salt-tolerant bacteria and biochar promotes grape growth under saline-alkaline stress","authors":"Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du","doi":"10.1016/j.eti.2025.104070","DOIUrl":"10.1016/j.eti.2025.104070","url":null,"abstract":"<div><div>Grapes (<em>Vitis vinifera</em>) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected <em>Bacillus subtilis</em> B4 and <em>Pseudomonas resinovorans</em> B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional <em>B. subtilis</em>, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104070"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0