Comamonas squillarum sp. nov., Isolated from Intestine of Red Swamp Crayfish (Procambarus clarkii).

IF 2.3 3区 生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2025-02-10 DOI:10.1007/s00284-025-04093-5
Shi Shi, Dao-Feng Zhang, Hong-Chuan Wang, Fu-Hui Jiang, Li-Fan Cui, Ying Huang
{"title":"Comamonas squillarum sp. nov., Isolated from Intestine of Red Swamp Crayfish (Procambarus clarkii).","authors":"Shi Shi, Dao-Feng Zhang, Hong-Chuan Wang, Fu-Hui Jiang, Li-Fan Cui, Ying Huang","doi":"10.1007/s00284-025-04093-5","DOIUrl":null,"url":null,"abstract":"<p><p>A novel bacterium, designated as strain PR12<sup>T</sup>, was isolated from intestine of red swamp crayfish (Procambarus clarkii), which appeared as creamy white colonies on TSA plates. Growth occurred at temperatures of 4-37 °C (28-30 °C optimal), pH of 6.0-9.0 (7.0-8.5 optimal), and with 0-4.0% (w/v) NaCl (0-1.5% optimal). The cells were Gram-stain-negative, rod-shaped, non-motile, aerobic, oxidase- and catalase-positive, and chemoorganotrophic. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PR12<sup>T</sup> was related to members of the genus Comamonas and shared the highest sequence similarities with Comamonas koreensis KCTC 12005<sup>T</sup> (99.0%), Comamonas sediminis S3<sup>T</sup> (98.8%), and Comamonas piscis CN1<sup>T</sup> (98.0%). Whole genome size of PR12<sup>T</sup> was 5,111,300 bp and DNA G + C content was 63.5%. The major cellular fatty acids (> 10%) were C<sub>16:0</sub>, C<sub>17:0</sub> cyclo, summed features 3 (C<sub>16:1</sub>ω6c and/or C<sub>16:1</sub>ω7c) and summed features 8 (C<sub>18:1</sub>ω6c and/or C<sub>18:1</sub>ω7c). The major respiratory quinone was Q-8 and the major polar lipids contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on the digital DNA-DNA hybridization, phylogenetic analysis, average nucleotide identity, average aminoacid identity as well as biochemical characteristics, strain PR12<sup>T</sup> was clearly distinguishable from all recognized type strains of the genus Comamonas and represents a novel species, for which the name Comamonas squillarum sp. nov. is proposed. The type strain is PR12<sup>T</sup> (= MCCC 1K08379<sup>T</sup> = JCM 35896<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"131"},"PeriodicalIF":2.3000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04093-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A novel bacterium, designated as strain PR12T, was isolated from intestine of red swamp crayfish (Procambarus clarkii), which appeared as creamy white colonies on TSA plates. Growth occurred at temperatures of 4-37 °C (28-30 °C optimal), pH of 6.0-9.0 (7.0-8.5 optimal), and with 0-4.0% (w/v) NaCl (0-1.5% optimal). The cells were Gram-stain-negative, rod-shaped, non-motile, aerobic, oxidase- and catalase-positive, and chemoorganotrophic. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PR12T was related to members of the genus Comamonas and shared the highest sequence similarities with Comamonas koreensis KCTC 12005T (99.0%), Comamonas sediminis S3T (98.8%), and Comamonas piscis CN1T (98.0%). Whole genome size of PR12T was 5,111,300 bp and DNA G + C content was 63.5%. The major cellular fatty acids (> 10%) were C16:0, C17:0 cyclo, summed features 3 (C16:1ω6c and/or C16:1ω7c) and summed features 8 (C18:1ω6c and/or C18:1ω7c). The major respiratory quinone was Q-8 and the major polar lipids contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on the digital DNA-DNA hybridization, phylogenetic analysis, average nucleotide identity, average aminoacid identity as well as biochemical characteristics, strain PR12T was clearly distinguishable from all recognized type strains of the genus Comamonas and represents a novel species, for which the name Comamonas squillarum sp. nov. is proposed. The type strain is PR12T (= MCCC 1K08379T = JCM 35896T).

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
从红色沼泽小龙虾(Procambarus clarkii)肠道中分离出一种新型细菌,命名为 PR12T 菌株,在 TSA 平板上呈乳白色菌落。生长温度为 4-37℃(最佳温度为 28-30℃),pH 值为 6.0-9.0(最佳 pH 值为 7.0-8.5),NaCl 含量为 0-4.0%(w/v)(最佳 NaCl 含量为 0-1.5%)。细胞呈革兰氏阴性,杆状,不运动,需氧,氧化酶和过氧化氢酶阳性,趋化有机营养型。基于 16S rRNA 基因序列的系统进化分析表明,PR12T 菌株与 Comamonas 属成员有亲缘关系,与 Comamonas koreensis KCTC 12005T (99.0%)、Comamonas sediminis S3T (98.8%)和 Comamonas piscis CN1T (98.0%)的序列相似度最高。PR12T 的全基因组大小为 5,111,300 bp,DNA G + C 含量为 63.5%。主要的细胞脂肪酸(> 10%)为 C16:0、C17:0 环、总特征 3(C16:1ω6c 和/或 C16:1ω7c)和总特征 8(C18:1ω6c 和/或 C18:1ω7c)。主要的呼吸醌是 Q-8,主要的极性脂质包括二磷脂酰甘油(DPG)、磷脂酰甘油(PG)和磷脂酰乙醇胺(PE)。根据数字 DNA-DNA 杂交、系统进化分析、平均核苷酸同一性、平均氨基酸同一性以及生化特征,PR12T 株明显有别于 Comamonas 属的所有已知模式菌株,是一个新物种,拟命名为 Comamonas squillarum sp.模式菌株为 PR12T(= MCCC 1K08379T = JCM 35896T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
期刊最新文献
Elucidating the Microsatellite Signature of the Tri-Partite Genomes of the Viral Family Peribunyaviridae. Genetic Diversity in Antimicrobial Resistance Determinants Among Pathogenic Pseudomonas aeruginosa in India. Statistical Optimization of Exopolysaccharide and Biomass Production by Mangrove Fungi Fusarium equiseti ANP2 and its Potential Application as Bioemulsifier and Chelator. Synergistic Antimicrobial Effects of Melaleuca alternifolia Essential Oil and Kojic Acid Combinations. Description of Cytobacillus Mangrovibacter sp. nov., and Cytobacillus Spartinae sp. nov., Isolated from Mangrove Sediment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1