Plant Growth-Promoting Potential of Colletotrichum sp. Isolated from Ocimum basilicum L. Leaves: A Broad-Spectrum Evaluation.

IF 2.6 3区生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2025-02-10 DOI:10.1007/s00284-025-04109-0

Karthika Manikandan, Rasmi Avanoor Ramanathan

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Abstract

The primary objective of this study was to assess the growth-promoting potential of Colletotrichum sp. as a broad-spectrum plant inoculant and to investigate its applicability beyond its natural host range. Out of ten endophytes isolated from Ocimum basilicum, only four were reported for IAA production and among them, Colletotrichum sp. was chosen for further investigation due to its higher IAA production. Highest production of IAA was observed by providing Czapek dox broth with dextrose as carbon source, ammonium sulphate as nitrogen source at pH 7 and 32 °C. The crude fungal extract from Colletotrichum sp. was tested for its impact on rice plants under different application methods (soil drenching, foliar spraying, and seed immersion). Seed immersion treatment showed a notable effect on the growth of rice seedlings in contrast to soil drenching and foliar spraying. Plant growth assay with fungal elicitor treatment of plantlets produced by shoot culture, supplementing with 0.04 mL of culture filtrate per 30 mL media has significant impact on induction of rooting and overall biomass in Ocimum tenuiflorum as compared to untreated control plants. High-resolution liquid chromatography mass spectrometry (HR-LCMS) analysis of the fungal extract revealed the production of 3-indoleacetic acid and related intermediates.

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从罗勒叶中分离的炭疽病菌促进植物生长的潜力：广谱评价。

本研究的主要目的是评估炭疽菌（Colletotrichum sp.）作为一种广谱植物接种剂的促生长潜力，并研究其在其自然寄主范围之外的适用性。从basilicum中分离到的10种内生菌中，仅有4种产IAA，其中因产IAA量较高，选择了Colletotrichum sp.作为进一步研究的对象。以葡萄糖为碳源、硫酸铵为氮源的Czapek还原肉汤在pH为7、温度为32℃条件下，IAA产量最高。以炭疽菌（Colletotrichum sp.）粗提物为研究对象，研究了不同施用方式（土壤淋施、叶面喷施和种子浸种）对水稻植株的影响。浸种处理对水稻幼苗生长的影响显著高于土壤淋施和叶面喷施。对茎部培养苗进行真菌激发剂处理，每30 mL培养基中添加0.04 mL培养滤液，与未经处理的对照植株相比，对山楂生根诱导和总生物量有显著影响。高分辨率液相色谱-质谱（HR-LCMS）分析表明真菌提取物产生3-吲哚乙酸和相关中间体。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.