Effect of curcumin nanoparticles on the number of preantral and antral follicles of white rats (Rattus norvegicus) exposed to carbon black.

IF 1 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI:10.5455/OVJ.2024.v14.i12.15

Jemy Caesar, Widjiati Widjiati, Eduardus Bimo Aksono Herupradoto, Moh Sukmanadi, Sri Pantja Madyawati, Hani Plumeriastuti, Epy Muhammad Luqman

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Abstract

Background: Continuous exposure to carbon black could affect the reproductive health of female animals, especially in the process of folliculogenesis. Curcumin nanoparticles are expected to maintain reproductive organ fertility through exposure to pollution containing carbon.

Aim: The purpose of this research is to investigate the effect of curcumin nanoparticles on the number of preantral and antral ovarian follicles in white rats (Rattus norvegicus) exposed to carbon black.

Methods: This study used 30 female rats divided into five groups. The negative (K-) and positive (K+) control groups were given aquades + Na-CMC 0.5% orally, while the treatment group received different doses of curcumin nanoparticles, namely, in the P1 (50 mg/kgBW), P2 (100 mg/kgBW), and P3 (150 mg/kgBW) groups, via oral administration. After that, K+, P1, P2, and P3 were exposed to carbon black with a concentration of 1064 mg/m³ for 6 hours/day for 30 days. The ovarian organ would then be made histopathological preparations to examine the number of preantral and antral follicles with HE staining.

Results: The results showed a significant difference (p < 0.05). The average number of preantral follicles in the K- group (27.17 ± 6.37), K+ (10.33 ± 6.22), P1 (26.17 ± 5.98), P2 (19.17 ± 3.71), and P3 (23.50 ± 10.36) (p < 0.05) along with the average number of antral follicles in the K- group (18.50 ± 8.89), K+ (9.17 ± 2.14), P1 (17.67 ± 7.45), P2 (16.00 ± 5.30), and P3 (9.50 ± 5.09).

Conclusion: The conclusion of this study shows that curcumin nanoparticles with different doses could affect and maintain preantral and antral ovarian follicles in white rats exposed to carbon black. It was also found that the dose of the P1 group (50 mg/kgBW) had the highest effectiveness in maintaining the number of ovarian follicles. The results of this study demonstrate the therapeutic potential of curcumin nanoparticles as an affordable drug.

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姜黄素纳米颗粒对暴露于炭黑的褐家鼠（Rattus norvegicus）腹前和腹前卵泡数量的影响。

背景：持续接触炭黑会影响雌性动物的生殖健康，尤其是在卵泡发生过程中。姜黄素纳米颗粒有望通过暴露于含碳污染中维持生殖器官的生育能力。目的：研究姜黄素纳米颗粒对炭黑作用下褐家鼠（Rattus norvegicus）腔前和腔前卵泡数量的影响。方法：选用30只雌性大鼠，分为5组。阴性（K-）和阳性（K+）对照组口服aquades + Na-CMC 0.5%，治疗组分别口服P1 （50 mg/kgBW）、P2 （100 mg/kgBW）和P3 （150 mg/kgBW）组不同剂量的姜黄素纳米颗粒。之后，K+、P1、P2和P3分别暴露于浓度为1064 mg/m3的炭黑中，每天6小时，共30天。然后对卵巢器官进行组织病理学准备，用HE染色检查腔前和腔前卵泡的数量。结果：两组比较差异有统计学意义（p < 0.05）。K-组平均窦前卵泡数（27.17±6.37）、K+（10.33±6.22）、P1（26.17±5.98）、P2（19.17±3.71）、P3(23.50±10.36)(p < 0.05)； K-组平均窦前卵泡数（18.50±8.89）、K+（9.17±2.14）、P1（17.67±7.45）、P2（16.00±5.30）、P3（9.50±5.09）。结论：不同剂量的姜黄素纳米颗粒对炭黑暴露大鼠的腔前和腔前卵泡均有影响和维持作用。还发现P1组（50 mg/kgBW）对维持卵巢卵泡数量的效果最高。本研究的结果表明，姜黄素纳米颗粒作为一种负担得起的药物具有治疗潜力。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.