Uric acid signal transduction enhancement through the electrical wiring of urate oxidase with copper Schiff base complex and reduced graphene oxide

IF 5.6 3区材料科学 Q1 ELECTROCHEMISTRY Electrochimica Acta Pub Date : 2025-02-11 DOI:10.1016/j.electacta.2025.145837

Teboho N. Moeketse , Priscilla G. Baker , Peter R. Makgwane

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Abstract

Uric acid (UA) is an important antioxidant biomolecule and disease biomarker in body fluids such as urine, sweat, serum, and saliva. In this paper, we developed an electrochemical biosensor to enhance UA signals using glassy carbon electrodes (GCEs) modified with graphene oxide, copper Schiff base complex and uricase enzyme. The reduced graphene oxide modified sensor took the lead by enhancing the UA signal response when coupled to Schiff base complex modified electrodes. The uricase enzyme biosensor yielded an extended linear range due to its catalytic effect on the UA oxidation process. The development of the sensors involved a step-by-step evaluation of the sensor performance using scanning mode and fixed potential mode electrochemistry, as well as spectroscopic and microscopic techniques. Square wave voltammetry in particular reported UA signal response in the linear range was 12 µM to 167 + µM, and LOD was in the range of 0.26 – 2.54 µM for UA sensors. This information is valuable for sensor application in human biofluids where UA concentrations may manifest up to 150–450 µM.

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通过铜席夫碱络合物和还原氧化石墨烯的尿酸氧化酶的电线增强尿酸信号转导

尿酸（UA）是尿液、汗液、血清和唾液等体液中重要的抗氧化生物分子和疾病生物标志物。在本文中，我们开发了一种电化学生物传感器，利用氧化石墨烯、铜希夫碱配合物和尿酸酶修饰的玻碳电极（GCEs）来增强UA信号。还原氧化石墨烯修饰的传感器在与希夫碱复合物修饰电极耦合时，通过增强UA信号响应而处于领先地位。尿酸酶生物传感器由于其对UA氧化过程的催化作用而产生了扩展的线性范围。传感器的开发涉及使用扫描模式和固定电位模式电化学以及光谱和微观技术逐步评估传感器性能。方波伏安法特别报道了UA信号在线性范围内的响应为12µM至167+µM， UA传感器的LOD在0.26 - 2.54µM范围内。这一信息对于传感器在人体生物体液中的应用是有价值的，其中UA浓度可能高达150-450µM。

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来源期刊

Electrochimica Acta 工程技术-电化学

CiteScore

11.30

自引率

6.10%

发文量

1634

审稿时长

41 days

期刊介绍： Electrochimica Acta is an international journal. It is intended for the publication of both original work and reviews in the field of electrochemistry. Electrochemistry should be interpreted to mean any of the research fields covered by the Divisions of the International Society of Electrochemistry listed below, as well as emerging scientific domains covered by ISE New Topics Committee.