In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications

IF 20.6 Q1 OPTICS Light-Science & Applications Pub Date : 2025-02-11 DOI:10.1038/s41377-024-01718-5

Hyejin Chang, Won Hur, Homan Kang, Bong-Hyun Jun

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Abstract

Surface-enhanced Raman scattering (SERS) has emerged as a powerful tool in various biomedical applications, including in vivo imaging, diagnostics, and therapy, largely due to the development of near-infrared (NIR) active SERS substrates. This review provides a comprehensive overview of SERS-based applications in vivo, focusing on key aspects such as the design considerations for SERS nanoprobes and advancements in instrumentation. Topics covered include the development of NIR SERS substrates, Raman label compounds (RLCs), protective coatings, and the conjugation of bioligands for targeted imaging and therapy. The review also discusses microscope-based configurations such as scanning, widefield imaging, and fiber-optic setups. Recent advances in using SERS nanoprobes for in vivo sensing, diagnostics, biomolecule screening, multiplex imaging, intraoperative guidance, and multifunctional cancer therapy are highlighted. The review concludes by addressing challenges in the clinical translation of SERS nanoprobes and outlines future directions, emphasizing opportunities for advancing biomedical research and clinical applications.

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表面增强拉曼散射（SERS）已成为各种生物医学应用（包括体内成像、诊断和治疗）的有力工具，这主要归功于近红外（NIR）活性 SERS 基底的开发。这篇综述全面概述了基于 SERS 的体内应用，重点关注 SERS 纳米探针的设计考虑因素和仪器的进步等关键方面。涉及的主题包括近红外 SERS 基底、拉曼标记化合物 (RLC)、保护涂层的开发，以及用于靶向成像和治疗的生物配体的共轭。综述还讨论了基于显微镜的配置，如扫描、宽场成像和光纤设置。重点介绍了将 SERS 纳米探针用于体内传感、诊断、生物分子筛选、多重成像、术中引导和多功能癌症治疗的最新进展。综述最后探讨了 SERS 纳米探针在临床应用中面临的挑战，并概述了未来的发展方向，强调了推动生物医学研究和临床应用的机遇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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