Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review

Abstract

Exopolysaccharides (EPS) produced by Bacillus species display various biological activities and characteristics such as anti-oxidant, immunomodulatory, anti-bacterial, and bioadhesive effects. These attributes confer Bacillus species broad potential applications in diverse fields such as food, medicine, environment, and agriculture. Moreover, Bacillus-derived EPS are easier to produce and yield higher quantities than plant-derived polysaccharides. Despite these advantages, Bacillus-derived EPS still encounter numerous obstacles in industrial production and commercial applications, including elevated costs, the absence of mature fermentation tank production procedures, and the lack of systematic in vivo and in vitro activity and metabolic evaluation. Therefore, it is essential to gain insight into the current status of structure, production, and applications of Bacillus-derived EPS for facilitating their future broader application. This paper provides a comprehensive overview of the current research on the production, separation, characteristics and applications of these related biological products. Furthermore, this paper summarizes the current challenges impeding industrial production of Bacillus-derived EPS, along with potential solutions, and their prospective applications in enhancing the attributes of beneficial biofilms, laying a solid scientific foundation for the applications of Bacillus-derived EPS in industry and agriculture.