Isolation and characterization of the novel Vibrio parahaemolyticus phage vB_VpaM_PVP-XSN

Abstract

A novel Vibrio parahaemolyticus phage, designated vB_VpaM_PVP-XSN (hereafter referred to as PVP-XSN), was isolated from costal soil. PVP-XSN has a polyhedral head (54.1 ± 11.1 nm in diameter) and a long contractile tail (110.5 ± 3.2 nm in length). The phage exhibits rapid adsorption and has a burst size of 167 plaque-forming units (PFU) per cell. Furthermore, PVP-XSN shows tolerance to temperatures ranging from 20°C to 50°C and pH levels between 5 and 11. Its linear double-stranded DNA genome is 45,651 base pairs in length and contains 66 putative open reading frames (ORFs). Comparative genomic analysis suggests that PVP-XSN represents a new genus of viruses.