Isolation and characterization of the novel Vibrio parahaemolyticus phage vB_VpaM_PVP-XSN

IF 2.5 4区 医学 Q3 VIROLOGY Archives of Virology Pub Date : 2025-02-11 DOI:10.1007/s00705-025-06237-1
Tianpeng Qi, Sunjian Lyu, Meiwen Xia, Bao Lou, Li Liu, Haisheng Xu
{"title":"Isolation and characterization of the novel Vibrio parahaemolyticus phage vB_VpaM_PVP-XSN","authors":"Tianpeng Qi,&nbsp;Sunjian Lyu,&nbsp;Meiwen Xia,&nbsp;Bao Lou,&nbsp;Li Liu,&nbsp;Haisheng Xu","doi":"10.1007/s00705-025-06237-1","DOIUrl":null,"url":null,"abstract":"<div><p>A novel <i>Vibrio parahaemolyticus</i> phage, designated vB_VpaM_PVP-XSN (hereafter referred to as PVP-XSN), was isolated from costal soil. PVP-XSN has a polyhedral head (54.1 ± 11.1 nm in diameter) and a long contractile tail (110.5 ± 3.2 nm in length). The phage exhibits rapid adsorption and has a burst size of 167 plaque-forming units (PFU) per cell. Furthermore, PVP-XSN shows tolerance to temperatures ranging from 20°C to 50°C and pH levels between 5 and 11. Its linear double-stranded DNA genome is 45,651 base pairs in length and contains 66 putative open reading frames (ORFs). Comparative genomic analysis suggests that PVP-XSN represents a new genus of viruses.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06237-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A novel Vibrio parahaemolyticus phage, designated vB_VpaM_PVP-XSN (hereafter referred to as PVP-XSN), was isolated from costal soil. PVP-XSN has a polyhedral head (54.1 ± 11.1 nm in diameter) and a long contractile tail (110.5 ± 3.2 nm in length). The phage exhibits rapid adsorption and has a burst size of 167 plaque-forming units (PFU) per cell. Furthermore, PVP-XSN shows tolerance to temperatures ranging from 20°C to 50°C and pH levels between 5 and 11. Its linear double-stranded DNA genome is 45,651 base pairs in length and contains 66 putative open reading frames (ORFs). Comparative genomic analysis suggests that PVP-XSN represents a new genus of viruses.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
新型副溶血性弧菌噬菌体 vB_VpaM_PVP-XSN 的分离和特征描述
从沿海土壤中分离到一种新的副溶血性弧菌噬菌体,命名为vB_VpaM_PVP-XSN(以下简称PVP-XSN)。PVP-XSN具有多面体头部(直径54.1±11.1 nm)和长尾(长度110.5±3.2 nm)。噬菌体表现出快速吸附,每个细胞有167个斑块形成单位(PFU)的爆发大小。此外,PVP-XSN对温度范围为20°C至50°C, pH值范围为5至11。其线性双链DNA基因组长度为45651个碱基对,包含66个假定的开放阅读框(orf)。比较基因组分析表明,PVP-XSN代表了一个新的病毒属。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
期刊最新文献
Genome characterization and environmental DNA-based detection of a novel adenovirus from red seabream (Pagrus major). Characterization and genomic analysis of a novel Vibrio harveyi Vibrio phage LRZ. Urine as a complementary specimen for RT-qPCR detection of Oropouche virus. Complete genome sequence of ramie marafivirus 1, a novel marafivirus infecting ramie (Boehmeria nivea). Viral genotype and the pace of epidemic waves: an assessment from SARS-CoV 2 genomics and wastewater surveillance data.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1