Pichia kudriavzevii (JD2) Immobilized on Acid Activated Perlite as a Biosorbent for Solid Phase Extraction of Cr(III) Determination by AAS (Atomic Adsorption Spectroscopy)

Abstract

In this study, the preconcentration of Cr(III) ion in Pichia kudriavzevii JD2 immobilized perlite adsorbent by solid phase extraction was investigated. The determination of Cr(III) was performed using flame atomic absorption spectrometry (AAS). The effects of pH, adsorbent amount, recovery solution volume and type, and sample solution flow rate and volume on the recovery efficiency of Cr(III) ions were investigated. Optimized preconcentration conditions for Cr(III) were established using a column technique. The optimal parameters were determined as pH 4, a recovery solution of 2 mol/L HNO₃ with a volume of 10 mL, and a sample flow rate of 1–3 mL/min, preconcentration factor 25. Under these conditions, the recovery efficiency of Cr(III) ion on perlite immobilized with Pichia kudriavzevii JD2 was found to be 100.1% ± 0.3% with a 95% confidence level. Analytical variables with a limit of detection (LOD) of 4.8 μg/L and a limit of quantification (LOQ) of 15.8 μg/L were determined for the Cr(III) ion. The accuracy of the method was determined using standard reference materials (SPS-WW1). The relative error of the recovery efficiency was determined to be less than 10%. The method was applied to the determination of Cr(III) in various water samples, such as tap water and mineral waters.