High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC) (Adv. Mater. 6/2025)
{"title":"High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC) (Adv. Mater. 6/2025)","authors":"Marieke Meteling, Castro Johnbosco, Alexis Wolfel, Francisco Conceição, Kannan Govindaraj, Liliana Moreira Teixeira, Jeroen Leijten","doi":"10.1002/adma.202570044","DOIUrl":null,"url":null,"abstract":"<p><b>Extracellular Protein Identification Cytometry</b></p><p>Knowledge of the extracellular matrix drives our understanding of cell behavior. However, current analysis methods are limited to either bulk or low-throughput single-cell analysis, thus masking the heterogeneity in matrix deposition. Extracellular protein identification cytometry (EPIC) combines the high-throughput single-cell analysis of flow cytometry with engineered microniche 3D cell culture, jointly enabling in situ matrix analysis of large cell populations. More details can be found in article number 2415981 by Jeroen Leijten and co-workers.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":114,"journal":{"name":"Advanced Materials","volume":"37 6","pages":""},"PeriodicalIF":26.8000,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adma.202570044","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Materials","FirstCategoryId":"88","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/adma.202570044","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Extracellular Protein Identification Cytometry
Knowledge of the extracellular matrix drives our understanding of cell behavior. However, current analysis methods are limited to either bulk or low-throughput single-cell analysis, thus masking the heterogeneity in matrix deposition. Extracellular protein identification cytometry (EPIC) combines the high-throughput single-cell analysis of flow cytometry with engineered microniche 3D cell culture, jointly enabling in situ matrix analysis of large cell populations. More details can be found in article number 2415981 by Jeroen Leijten and co-workers.
期刊介绍：
Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
