Gut microbiota and metabolic profile affected by pectic domains during in vitro rat fecal fermentation: A comparative study between different glycans rich in pectic monosaccharides

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2025-02-09 DOI:10.1016/j.carbpol.2025.123365

Yuanyuan Zhao , Pan Wang , Dan Wang , Wenting Zhao , Junjuan Wang , Zhiwen Ge , Ye Liu , Xiaoyan Zhao

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Abstract

This study aimed to investigate in vitro rat fecal fermentation behavior of pectic polymers and glycans that constitute typical pectic fragments, i.e. homogalacturonan (HG), arabinan (AB), arabinogalactan (AG), rhamnogalacturonan (RG), and xyloglucan (XG). Results showed that galacturonic acid proportion of HG (73.85 mol%) was the highest, followed by pectin (67.99 mol%), whereas arabinose (70.23 mol%) and galactose (86.22 mol%) enriched in AB and AG, respectively. Absolute quantitative microbiome revealed that Bacteroides showed dramatic growth in RG and AG; higher absolute abundances of Bifidobacterium (5.06E+09 and 3.36E+09 copies/g feces, respectively) were found in AB and XG; Escherichia Shigella, Enterococcus, and Klebsiella were inhibited after fermentation with pectin and HG by >95 %. Untargeted metabolomics indicated that the differential metabolite in AG and RG were 7-ketodeoxycholic acid and 9,10-epoxyoctadecanoic acid, respectively, both of which were positively related to arabinose and galactose (p < 0.001). Besides, another characteristic monosaccharide, rhamnose was positively correlated with succinic acid (p < 0.05), and Parvibacter (p < 0.001). Overall, this work help to understand the interactions among pectin structure, gut microbiota and metabolites, thereby guiding the targeted design of the nutrient-directed pectins in future personalized diets.

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大鼠粪便体外发酵过程中受果胶结构域影响的肠道微生物群和代谢谱：富含果胶单糖的不同聚糖的比较研究

本研究旨在研究构成典型果胶片段的果胶聚合物和聚糖，即均半乳糖酸（HG）、阿拉伯糖酸（AB）、阿拉伯半乳糖酸（AG）、鼠李糖半乳糖酸（RG）和木葡聚糖（XG）在体外大鼠粪便中的发酵行为。结果表明，HG中半乳糖醛酸含量最高（73.85 mol%），果胶含量次之（67.99 mol%）， AB和AG中分别富含阿拉伯糖（70.23 mol%）和半乳糖（86.22 mol%）。微生物组绝对数量显示，拟杆菌在RG和AG中显著增长；AB组和XG组双歧杆菌绝对丰度较高（分别为5.06E+09和3.36E+09拷贝/g）；经果胶和HG发酵后，志贺氏杆菌、肠球菌和克雷伯氏菌的抑制率达95%。非靶向代谢组学表明，AG和RG的差异代谢物分别为7-酮去氧胆酸和9,10-环氧十八烷酸，两者均与阿拉伯糖和半乳糖呈正相关(p <；0.001)。此外，鼠李糖与琥珀酸呈显著正相关(p <；0.05)，细小杆菌(p <；0.001)。总的来说，这项工作有助于了解果胶结构、肠道微生物群和代谢物之间的相互作用，从而指导未来个性化饮食中营养导向果胶的有针对性设计。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.