Digital microfluidic-based fluorescence methods for the automated determination of copper ions in wine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-13 DOI:10.1007/s00604-025-07019-x

Zhihui Liu, Si Wang, Kemin Wang, Jiajun Tong, Zijun Zhao, Xiaofeng Liu, Yiwei Liu

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Abstract

A novel approach is introduced by combining digital microfluidic technology with click chemistry for automated sample handling on a chip, enabling accurate detection of copper ions in wine. By developing a copper-catalyzed click chemistry reaction using azide coumarin and hexanol, we have introduced a method that offers advantages such as simplicity, minimal by-products, and enhanced resistance to interference compared with other fluorescent methods. Furthermore, optimization of the digital microfluidic chip parameters enabled processing of sub-microliter samples with a droplet coefficient of variation of 0.6%, outperforming the ~ 4.0% error typically seen with conventional pipetting methods. This method processes samples as small as 870 nL, providing cost efficiency, automated detection, reduced errors, and a detection limit of 15.4 μM (0.98 mg/L), meeting testing requirements. Our approach effectively detects copper ion contamination in wine with a recovery of 98.7 to 106%, offering robust technical support for food safety regulations.

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数字微流控荧光法自动测定葡萄酒中铜离子

介绍了一种将数字微流体技术与点击化学相结合的新方法，用于芯片上的自动样品处理，能够准确检测葡萄酒中的铜离子。通过利用叠氮香豆素和己醇开发一种铜催化的点击化学反应，我们介绍了一种与其他荧光方法相比具有简单、副产物最少和增强抗干扰性等优点的方法。此外，通过优化数字微流控芯片参数，处理亚微升样品的液滴变异系数为0.6%，优于传统移液方法通常出现的~ 4.0%的误差。该方法可处理小至870 nL的样品，具有成本效益高、检测自动化、误差小的特点，检出限为15.4 μM (0.98 mg/L)，满足测试要求。该方法可有效检测葡萄酒中的铜离子污染，回收率为98.7% ~ 106%，为食品安全监管提供有力的技术支持。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.