Different Transcriptome Signatures of the Lymphatic and the Blood Vessels From Rat Mesentery Reveal Distinct Function Characteristics

IF 1.9 4区医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2025-02-13 DOI:10.1111/micc.70003

Yumeng Jing, Jiayi Zhai, Min Gao, Xiu Xu, Zi-Gang Zhao, Zhen-Ao Zhao

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引用次数: 0

Abstract

Objective

Lymphatic vessels and blood vessels have some similarities in structure, but they have distinct contraction characteristics and functions. Revealing the detailed transcriptional differences of lymphatic, artery and vein are required for circulation research.

Methods

The tissues of the mesenteric lymphatic, artery, and vein were collected from Wistar rats. The transcriptome signatures of these tissues from RNA-seq (RNA sequencing) were analyzed using bioinformatic methods.

Results

GO (gene ontology) enrichment showed the three tissues have distinct gene expression patterns in extracellular matrix, cell adhesion molecule binding, receptor ligand activity, and contractile fiber. The genes involved in cell contractility were also differently expressed, which were enriched into the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of cytoskeleton in muscle cells, vascular smooth muscle contraction, and renin-angiotensin system. Through PPI (protein–protein interaction) analysis, we identified 43 differently expressed hub genes in the three tissues. Thirty-four transcription factors and cofactors were identified as important for the normal function of the three tissues. Furthermore, we screened out 20 potential marker genes for each tissue.

Conclusions

Our study described the transcriptome signatures of mesenteric lymphatic, artery, and vein, shedding light on the distinct contraction mechanisms of these tissues. These results also provided potential therapeutic targets for circulation diseases and potential markers for lymphatic and blood vessel studies.

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.