Correlation Between miR-27a-3p Polymorphisms and Peri-Implantitis Susceptibility: A Case-Control Study.

Abstract

Background: Peri-implantitis (PI) is characterised by a chronic inflammatory response that affects both the soft and bone tissues around dental implants. Inflammatory condition, coupled with alterations in bone metabolism, may be modulated by miR-27a-3p.

Aim: This research aims to investigate the relationship between miR-27a-3p rs895819 polymorphism with PI susceptibility, along with its effects and mechanism on inflammatory factors.

Methods: Relative miR-27a-3p level was assessed using reverse transcription-polymerase chain reaction (RT-PCR). rs895819 was genotyped by restriction fragment length polymorphism. Enzyme-linked immunosorbent assay (ELISA) kits detected the concentrations of RUNX1, C-reactive protein (CRP), and interleukin-6 (IL-6). Overlapping target genes of miR-27a-3p were identified from databases, including ENCORI, miRDB, and TargetScan databases, which facilitated the selection of target genes of miR-27a-3p. Overlap genes were compiled by the Venn diagram. Functional enrichment analysis for target genes was performed via Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) networks.

Results: Underexpressed miR-27a-3p was discovered in PI patients. GG genotype (crude: odds ratio (OR) = 2.898, 95% confidence interval (CI) = 1.419-5.917; adjusted: OR = 2.157, 95% CI = 1.045-4.455) and G allele (P = .003, OR = 1.501, 95% CI = 1.144-1.968) of rs895819 demonstrates a significant association with an enhanced PI susceptibility. The direct target gene RUNX1 has been identified from these databases. PI patients possessing rs895819 GG genotype exhibit diminished level of miR-27a-3p and elevated concentrations of RUNX1 (P < .05), CRP (P < .001), IL-6 (P < .001), and white blood cell (WBC) count (P < .05).

Conclusion: These results suggest that rs895819 of miR-27a-3p serves as a significant risk predictor for PI patients. This variant is associated with decreased miR-27a-3p levels, alongside elevated RUNX1 levels and inflammatory factor levels in PI patients.