High concentration of phosphate treatment increased the tolerance of Robinia pseudoacacia roots to salt stress.

Abstract

Key message: High P increased the tolerance of R. pseudoacacia roots to salt stress. Salt is an important abiotic factor that restricts plant growth and development in soil. An appropriate concentration of P can increase plant tolerance to salt stress. We investigated the physiological and transcriptional regulatory effects of high P (HP) or low P (LP) on the response of R. pseudoacacia roots to salt stress. A pot experiment was carried out to grow R. pseudoacacia seedlings in vermiculite media supplemented with 0 mM, 150 mM or 300 mM NaCl under HP or LP conditions. The root dry weight and concentrations of free proline, P, ions, and phytohormones were measured, and the transcription of the genes was analyzed under NaCl stress under HP or LP conditions. The results revealed that R. pseudoacacia responds to NaCl stress by regulating the absorption and utilization of P and the levels of free proline, phytohormones and Na⁺, K⁺, Ca²⁺, and Mg²⁺ as well as changing the expression levels of key genes. Compared with those under the LP condition, the roots of the R. pseudoacacia under the HP condition presented greater P concentrations, lower JA concentrations, and more stable K⁺ levels when subjected to NaCl stress, which increased their tolerance to NaCl stress. Moreover, genes involved in the cell wall, root growth, root architecture regulation, biomass accumulation, stress response, osmotic regulation and ion balance maintenance were upregulated under NaCl stress under HP conditions. In addition, NaCl stress impairs N metabolism under LP conditions. Our findings provide new insights into the response of woody plants to salt stress under different P conditions and contribute to the development of scientific afforestation in saline-alkali areas.